{"id":6888,"date":"2024-02-25T11:32:51","date_gmt":"2024-02-25T10:32:51","guid":{"rendered":"https:\/\/www.em-grade.com\/general-de\/rezept-zubereitung-pbs-phosphatpuffersalzloesung-1x-ph-74\/"},"modified":"2024-02-25T11:32:51","modified_gmt":"2024-02-25T10:32:51","slug":"rezept-zubereitung-pbs-phosphatpuffersalzloesung-1x-ph-74","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/de\/puffer\/rezept-zubereitung-pbs-phosphatpuffersalzloesung-1x-ph-74\/","title":{"rendered":"Rezept \/ Zubereitung : PBS (Phosphatpuffersalzl\u00f6sung) 1X, pH 7,4."},"content":{"rendered":"

Zur Vorbereitung von 500 ml PBS (1X, pH 7,4)<\/p>\n\n\n\n\n\n\n\n
Komponente<\/td>\nBetrag<\/td>\nKonzentration<\/td>\n<\/tr>\n
Natriumchlorid<\/a> (mw: 58.44 g\/mol)<\/td>\n4 g<\/td>\n0.137 M<\/td>\n<\/tr>\n
Kaliumchlorid (mw: 74.55 g\/mol)<\/td>\n0.1 g<\/td>\n 0.0027 M<\/td>\n<\/tr>\n
Natriumphosphat dibasisch (mw: 141.96 g\/mol)<\/td>\n0.72 g<\/td>\n 0.01 M<\/td>\n<\/tr>\n
Kaliumphosphat einbasisch (mw: 136.09 g\/mol)<\/td>\n 0.1225 g<\/td>\n0.0018 M<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n


\n\"\"<\/a><\/p>\n

    \n
  1. Bereiten Sie 450 mL destilliertes Wasser in einem geeigneten Gef\u00e4\u00df vor.<\/li>\n
  2. 4 g Natriumchlorid in die L\u00f6sung geben.<\/li>\n
  3. 0,1 g Kaliumchlorid in die L\u00f6sung geben.<\/li>\n
  4. 0,72 g Natriumphosphat dibasisch zu der L\u00f6sung hinzuf\u00fcgen.<\/li>\n
  5. 0,1225 g Kaliumphosphat einbasisch zu der L\u00f6sung hinzuf\u00fcgen.<\/li>\n
  6. L\u00f6sung auf das gew\u00fcnschte Ma\u00df einstellen<\/a> pH \u2248 7,4 oder anderer pH-Wert.<\/li>\n
  7. Destilliertes Wasser hinzuf\u00fcgen, bis das Volumen 500 ml betr\u00e4gt.<\/li>\n<\/ol>\n

    Wir bieten auch unseren gebrauchsfertigen PBS-Puffer an, der in unserem Shop erh\u00e4ltlich ist: PBS-Puffer. <\/a><\/h4>\n","protected":false},"excerpt":{"rendered":"

    Zur Vorbereitung von 500 ml PBS (1X, pH 7,4) Komponente Betrag Konzentration Natriumchlorid (mw: 58.44 g\/mol) 4 g 0.137 M Kaliumchlorid (mw: 74.55 g\/mol) 0.1 g 0.0027 M Natriumphosphat dibasisch…<\/p>\n","protected":false},"author":8,"featured_media":6517,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[444],"tags":[635],"class_list":["post-6888","post","type-post","status-publish","format-standard","has-post-thumbnail","category-puffer","tag-rezept-pbs"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts\/6888","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/comments?post=6888"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts\/6888\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/media\/6517"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/media?parent=6888"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/categories?post=6888"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/tags?post=6888"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}