{"id":7129,"date":"2024-01-19T17:03:56","date_gmt":"2024-01-19T16:03:56","guid":{"rendered":"https:\/\/www.em-grade.com\/general-de\/phem-puffer-rezeptur\/"},"modified":"2024-01-19T17:03:56","modified_gmt":"2024-01-19T16:03:56","slug":"phem-puffer-rezeptur","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/de\/puffer\/phem-puffer-rezeptur\/","title":{"rendered":"PHEM-Puffer-Rezeptur"},"content":{"rendered":"

PHEM-Puffer-Rezeptur<\/em><\/h2>\n

PHEM-Puffer wurde haupts\u00e4chlich f\u00fcr Gewebe und Zellkulturen verwendet, die f\u00fcr immunzytochemische Untersuchungen aufbereitet werden. Die meisten Antigene, insbesondere die intrazellul\u00e4ren, f\u00e4rben sich mit PHEM besser als mit PBS, und es hat auch eine geringere Wirkung auf biochemische Reaktionen und Enzyme. Die sehr gute Erhaltung der zellul\u00e4ren Ultrastruktur, insbesondere der Mikrotubuli, ist ebenfalls ein Vorteil der Verwendung von PHEM.<\/p>\n

\"\"<\/a><\/strong><\/p>\n

Referenzen:<\/strong><\/p>\n

    \n
  1. Schliwa, Manfred, and Jonathan Van Blerkom<\/strong><\/em>. „Structural interaction of cytoskeletal components.“ The Journal of Cell Biology 90.1 (1981): 222-235.<\/li>\n
  2. Jacqueline Montanaro<\/em><\/strong>: Verbesserte Ultrastruktur von wirbellosen Meerestieren unter Verwendung von nicht-toxischen Puffern<\/li>\n<\/ol>\n

    <\/h4>\n

    Zubereitungsrezept f\u00fcr 500 ml Puffer PHEM 0,2 M <\/u><\/strong><\/span><\/h4>\n

    L\u00f6sen Sie das Reagenzienpulver in 400 ml destilliertem Wasser in folgender Reihenfolge auf:<\/em><\/p>\n\n\n\n\n\n\n\n\n
    Reagenzienpulver<\/strong><\/td>\nNummer C.A.S<\/strong><\/td>\nGewicht Gramm<\/strong><\/td>\nRef. em-grade<\/strong><\/td>\n<\/tr>\n
    HEPES<\/strong><\/td>\n7365-45-9<\/td>\n0.598 gr.<\/td>\nGP19<\/a><\/strong><\/td>\n<\/tr>\n
    EGTA<\/strong><\/td>\n67-42-5<\/td>\n0.380 gr.<\/td>\nGP27<\/strong><\/td>\n<\/tr>\n
    MgSO4 , 7H2O<\/strong><\/td>\n10034-99-8<\/td>\n0.098 gr<\/td>\nGP28<\/strong><\/td>\n<\/tr>\n
    P.I.P.E.S.<\/strong><\/td>\n5625-37-6<\/td>\n1.814 gr<\/td>\nGP21<\/a><\/strong><\/td>\n<\/tr>\n
    WASSER D<\/em>.<\/td>\nAuf 500 ml Endvolumen auff\u00fcllen und mit NaOH auf den gew\u00fcnschten pH-Wert einstellen<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    Filtern Sie die L\u00f6sung auf 0,22\u00b5.<\/em><\/p>\n

    Wir bieten auch unseren gebrauchsfertigen PHEM-Puffer<\/a> in unserem Shop an: PHEM-Puffer. <\/span><\/h4>\n

    Ref. :GT140000<\/span><\/a><\/span><\/span><\/h4>\n","protected":false},"excerpt":{"rendered":"

    PHEM-Puffer-Rezeptur PHEM-Puffer wurde haupts\u00e4chlich f\u00fcr Gewebe und Zellkulturen verwendet, die f\u00fcr immunzytochemische Untersuchungen aufbereitet werden. Die meisten Antigene, insbesondere die intrazellul\u00e4ren, f\u00e4rben sich mit PHEM besser als mit PBS, und…<\/p>\n","protected":false},"author":8,"featured_media":7130,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[444],"tags":[640],"class_list":["post-7129","post","type-post","status-publish","format-standard","has-post-thumbnail","category-puffer","tag-phem-puffer"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts\/7129","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/comments?post=7129"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts\/7129\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/media\/7130"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/media?parent=7129"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/categories?post=7129"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/tags?post=7129"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}