{"id":7249,"date":"2023-11-10T11:14:41","date_gmt":"2023-11-10T10:14:41","guid":{"rendered":"https:\/\/www.em-grade.com\/general-de\/der-imidazolpuffer-ermoeglicht-die-rueckhaltung-von-lipiden\/"},"modified":"2023-11-10T11:14:41","modified_gmt":"2023-11-10T10:14:41","slug":"der-imidazolpuffer-ermoeglicht-die-rueckhaltung-von-lipiden","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/de\/puffer\/der-imidazolpuffer-ermoeglicht-die-rueckhaltung-von-lipiden\/","title":{"rendered":"Der Imidazolpuffer erm\u00f6glicht die R\u00fcckhaltung von Lipiden"},"content":{"rendered":"

Der Imidazolpuffer erm\u00f6glicht die Erhaltung der am schwierigsten zu konservierenden intrazellul\u00e4ren Lipide<\/strong>.<\/p>\n

Der Imidazolpuffer erm\u00f6glicht die R\u00fcckhaltung von Lipiden<\/u><\/strong>. in combination with osmium during fixation as a buffer at 0.1 M<\/p>\n

Hier ist ein Standardprotokoll<\/p>\n

    \n
  1. Glutaraldehyd ‚2 bis 2,5% in 0,1M Imidazolpuffer<\/strong> pH7,5 – RT<\/li>\n
  2. Waschen in 0,2 M Imidazolpuffer<\/strong> pH 7,5 (2 B\u00e4der 3 Minuten) bei RT<\/li>\n
  3. Osmium 1% bis 2% in Imidazolpuffer 0,1<\/strong> pH 7,5, 30 min – RT<\/li>\n
  4. Letzter Waschgang wie unter Punkt 2 (Waschen in 0,2 M Imidazolpuffer pH 7,5 (2 B\u00e4der, nicht mehr, 3 Minuten) bei RT<\/li>\n<\/ol>\n

    Ersetzen Sie Ihre \u00fcblichen Sorensen-PBS- oder Kakodylat-Puffer durch Imidazol, wenn in Ihrer Studie Lipideinschl\u00fcsse von Bedeutung sind.<\/p>\n

    Wichtiger Hinweis: Wenn Sie den Inhalt der fixierten Zellen erhalten wollen, sollten Sie nicht zu viel waschen, 1 Waschgang reicht aus; wenn einfache Lipide am schwierigsten zu erhalten sind, sollte das Waschen sogar vermieden werden.<\/p>\n

    \"\" <\/a> \"Imidazol-Puffer-Lipid-Konservierung\"<\/a><\/p>\n

    Hepatozytenzelle<\/p>\n","protected":false},"excerpt":{"rendered":"

    Der Imidazolpuffer erm\u00f6glicht die Erhaltung der am schwierigsten zu konservierenden intrazellul\u00e4ren Lipide. Der Imidazolpuffer erm\u00f6glicht die R\u00fcckhaltung von Lipiden. in combination with osmium during fixation as a buffer at 0.1…<\/p>\n","protected":false},"author":8,"featured_media":7251,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[444],"tags":[673,674],"class_list":["post-7249","post","type-post","status-publish","format-standard","has-post-thumbnail","category-puffer","tag-imidazol-puffer","tag-lipide"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts\/7249","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/comments?post=7249"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts\/7249\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/media\/7251"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/media?parent=7249"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/categories?post=7249"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/tags?post=7249"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}