{"id":7333,"date":"2023-11-17T11:33:02","date_gmt":"2023-11-17T10:33:02","guid":{"rendered":"https:\/\/www.em-grade.com\/general-de\/em-faerbung-ote-oolong-tea-zur-faerbung-von-ultraduennschnitten\/"},"modified":"2023-11-17T11:33:02","modified_gmt":"2023-11-17T10:33:02","slug":"em-faerbung-ote-oolong-tea-zur-faerbung-von-ultraduennschnitten","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/de\/em-flecken\/em-faerbung-ote-oolong-tea-zur-faerbung-von-ultraduennschnitten\/","title":{"rendered":"EM-F\u00e4rbung OTE (OOLONG TEA) zur F\u00e4rbung von Ultrad\u00fcnnschnitten."},"content":{"rendered":"<p>Vorbereitung und Verwendung des O.T.E.<\/p>\n<p>Die Zubereitung der OTE-L\u00f6sung ist \u00e4hnlich wie der Aufguss von Teewasser 70\u00b0C oder Puffer 0,1M.(*)<\/p>\n<p>&#8211; L\u00f6sen Sie Ihr OTE-Pulver mit einem auf 70\u00b0C eingestellten Heizmagnetr\u00fchrer in einer Konzentration von 0,2% auf (da die S\u00e4ttigung bei 0,3% erreicht wird)<\/p>\n<p>&#8211; Nach vollst\u00e4ndiger Aufl\u00f6sung (15 bis 30 Minuten unter R\u00fchren bei 70 \u00b0 C),<\/p>\n<ol>\n<li>Filtern Sie Ihre OTE-Extraktl\u00f6sung bei 0,45\u00b5.<\/li>\n<li>In einem kleinen Eppendorf-R\u00f6hrchen, das maximal gef\u00fcllt ist, um sauerstoffhaltige Luft zu reduzieren, oder in einer AirLess-Flasche aufbewahren.<\/li>\n<li>Im Dunkeln bei 4 \u00b0C aufbewahren (bei der Handhabung die L\u00f6sungen immer auf RT zur\u00fcckkehren lassen)<\/li>\n<li>Kontrastieren Sie Ihre Gitter mit OTE f\u00fcr 15 bis 25 Minuten, Dunkelheit, RT<\/li>\n<li>Einfach abtropfen lassen, nicht aussp\u00fclen<\/li>\n<li>Nachf\u00e4rben 1 bis 2 Minuten mit Bleizitrat nach Reynolds (Ref. 11300 von Delta Microscopies)<\/li>\n<li>Abgie\u00dfen, kurz absp\u00fclen, abtrocknen und fertig zum Anschauen.<\/li>\n<\/ol>\n<p>*Anmerkung: Die L\u00f6sung kann nicht l\u00e4nger als eine Woche aufbewahrt werden, aber mehr Zeit in einer Kakodylat-Pufferl\u00f6sung, AirLess-Flasche (kein Bakterienwachstum).<\/p>\n<p><a href=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/11\/OTE-EM-stainfollicular-cell.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1817\" src=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/11\/OTE-EM-stainfollicular-cell-150x150.jpg\" alt=\"\" width=\"297\" height=\"297\" srcset=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/11\/OTE-EM-stainfollicular-cell-150x150.jpg 150w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/11\/OTE-EM-stainfollicular-cell-300x300.jpg 300w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/11\/OTE-EM-stainfollicular-cell-140x140.jpg 140w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/11\/OTE-EM-stainfollicular-cell-100x100.jpg 100w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/11\/OTE-EM-stainfollicular-cell.jpg 322w\" sizes=\"auto, (max-width: 297px) 100vw, 297px\" \/>                <\/a><a href=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/11\/OTE-EM-stain-spermatide.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1816\" src=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/11\/OTE-EM-stain-spermatide-150x150.jpg\" alt=\"\" width=\"292\" height=\"292\" srcset=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/11\/OTE-EM-stain-spermatide-150x150.jpg 150w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/11\/OTE-EM-stain-spermatide-140x140.jpg 140w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/11\/OTE-EM-stain-spermatide-100x100.jpg 100w\" sizes=\"auto, (max-width: 292px) 100vw, 292px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Vorbereitung und Verwendung des O.T.E. Die Zubereitung der OTE-L\u00f6sung ist \u00e4hnlich wie der Aufguss von Teewasser 70\u00b0C oder Puffer 0,1M.(*) &#8211; L\u00f6sen Sie Ihr OTE-Pulver mit einem auf 70\u00b0C eingestellten&#8230;<\/p>\n","protected":false},"author":8,"featured_media":7263,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[446],"tags":[688,687],"class_list":["post-7333","post","type-post","status-publish","format-standard","has-post-thumbnail","category-em-flecken","tag-oolong-tee-extrakt","tag-ote-de"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts\/7333","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/comments?post=7333"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts\/7333\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/media\/7263"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/media?parent=7333"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/categories?post=7333"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/tags?post=7333"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}