{"id":7339,"date":"2023-11-20T15:36:13","date_gmt":"2023-11-20T14:36:13","guid":{"rendered":"https:\/\/www.em-grade.com\/general-de\/phem-buffer-der-beste-puffer-fuer-immunfluoreszenzzellkulturen\/"},"modified":"2023-11-20T15:36:13","modified_gmt":"2023-11-20T14:36:13","slug":"phem-buffer-der-beste-puffer-fuer-immunfluoreszenzzellkulturen","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/de\/puffer\/phem-buffer-der-beste-puffer-fuer-immunfluoreszenzzellkulturen\/","title":{"rendered":"PHEM BUFFER , der beste Puffer f\u00fcr Immunfluoreszenzzellkulturen."},"content":{"rendered":"

Der PHEM-Puffer<\/strong> wurde zur Konservierung von Mikrotubuli entwickelt und wird zur Konservierung von Zellen verwendet.<\/span> <\/span>Struktur in der Immunf\u00e4rbung<\/span><\/p>\n

Referenzen<\/strong>: Schliwa, Manfred, und Jonathan Van Blerkom. „Structural interaction of cytoskeletal components.“ The Journal of Cell Biology 90.1 (1981): 222-235. <\/span><\/em><\/p>\n

PHEM-Puffer wird f\u00fcr immunzytochemische Untersuchungen und Immunf\u00e4rbungen von Gewebe- und Zellkulturen empfohlen.<\/p>\n

\"\" <\/a>\"\"<\/a><\/p>\n

Laboruntersuchungen haben gezeigt, dass der PHEM-Puffer eine bessere Erkennung intrazellul\u00e4rer Antigene und eine geringere Beeintr\u00e4chtigung enzymatischer und biochemischer Reaktionen erm\u00f6glicht, w\u00e4hrend PBS spezifischer f\u00fcr extrazellul\u00e4re Membranantigene ist; aus diesem Grund wird PHEM bei Fluoreszenzmarkierungen bevorzugt;<\/p>\n

Die Verwendung von PHEM gew\u00e4hrleistet auch eine hervorragende Erhaltung der zellul\u00e4ren Ultrastruktur, insbesondere der Mikrotubuli. Der als CSK bekannte Puffer, ein Derivat der PHEM-Formulierung, wird C<\/strong>ytoSK<\/strong>eletal (CSK)-Puffer genannt und scheint die Mikrotubuli-Markierung zu verbessern. <\/p>\n

 <\/p>\n

weitere Informationen<\/strong><\/span> PHEM-Puffer Rezeptur<\/a><\/h3>\n","protected":false},"excerpt":{"rendered":"

Der PHEM-Puffer wurde zur Konservierung von Mikrotubuli entwickelt und wird zur Konservierung von Zellen verwendet. Struktur in der Immunf\u00e4rbung Referenzen: Schliwa, Manfred, und Jonathan Van Blerkom. „Structural interaction of cytoskeletal…<\/p>\n","protected":false},"author":8,"featured_media":7274,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[444],"tags":[695,696],"class_list":["post-7339","post","type-post","status-publish","format-standard","has-post-thumbnail","category-puffer","tag-em-stain-de","tag-phem-de"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts\/7339","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/comments?post=7339"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/posts\/7339\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/media\/7274"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/media?parent=7339"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/categories?post=7339"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/tags?post=7339"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}