{"id":4976,"date":"2023-12-04T16:35:57","date_gmt":"2023-12-04T15:35:57","guid":{"rendered":"https:\/\/www.em-grade.com\/product\/10-neutral-buffered-formalin-nbf\/"},"modified":"2024-03-07T10:44:54","modified_gmt":"2024-03-07T09:44:54","slug":"10-neutral-buffered-formalin-nbf-for-pathology-lab","status":"publish","type":"product","link":"https:\/\/www.em-grade.com\/de\/produkt\/10-neutral-buffered-formalin-nbf-for-pathology-lab\/","title":{"rendered":"Neutral Buffered Formalin (NBF) 10%"},"content":{"rendered":"<p><strong>Product Description:<\/strong><\/p>\n<p>Neutral-buffered formalin (NBF) is a commonly used fixative in histology for light microscopy only.<br \/>\nComposition: NBF is composed of formaldehyde (usually at a concentration of 10%, which corresponds to approximately 4% formaldehyde by volume) diluted with phosphate buffer to achieve a neutral pH. The phosphate buffer helps maintain the pH stability of the solution.<\/p>\n<p><strong>Application:<\/strong><\/p>\n<p>NBF serves as a fixative for preserving tissue samples. It is widely employed in preparing samples for light microscopy. Here are some key points about NBF:<\/p>\n<ul>\n<li><strong><em>Fixation<\/em>:<\/strong> When tissue is immersed in NBF, formaldehyde reacts with proteins, creating a gel-like structure that retains cellular constituents in their in vivo relationship. Properly fixed tissue can withstand subsequent stages of tissue processing and staining.<\/li>\n<li><strong><em>Applications<\/em>:<\/strong>\n<ul>\n<li>Histology: NBF is a popular general-purpose histological fixative used to fix tissues for microscopic examination.<\/li>\n<li>Staining Techniques: It has been used in various staining techniques, including oil-red-O staining for differentiating preadipocyte cell lines and analyzing tissues like rabbit eyes and diabetic rat intestines.<\/li>\n<li>Storage: NBF is suitable for both short-term fixation (useful for high-throughput laboratories) and long-term storage of tissue samples.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><strong>Tip:<br \/>\n<\/strong>NBF is labeled with hazard statements related to toxicity, skin sensitivity, and carcinogenicity. Proper precautions should be taken when handling this solution.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Phosphate Buffered Only for Histology , light Microscopy<\/p>\n<ul>\n<li>not used electron microscopy fixation<\/li>\n<li>pH 6.9 to 7.1<\/li>\n<li>distilled water, phosphate buffer<\/li>\n<li>store at room temperature (RT)<\/li>\n<li>made <span class=\"inner\">in<\/span>\u00a0<span class=\"inner\">France<\/span>\n<div class=\"divider-wrap\" data-alignment=\"default\"><\/div>\n<\/li>\n<\/ul>\n","protected":false},"featured_media":4977,"template":"","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":""},"product_brand":[],"product_cat":[391,392],"product_tag":[],"class_list":["post-4976","product","type-product","status-publish","has-post-thumbnail","product_cat-fixiermittel","product_cat-histologie-de","pa_volume-1000ml-de","pa_volume-500ml-de","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/product\/4976","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/media\/4977"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/media?parent=4976"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/product_brand?post=4976"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/product_cat?post=4976"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/product_tag?post=4976"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}