{"id":6128,"date":"2023-12-04T16:35:00","date_gmt":"2023-12-04T15:35:00","guid":{"rendered":"https:\/\/www.em-grade.com\/product\/sorensens-phosphate-buffer\/"},"modified":"2025-05-15T15:12:16","modified_gmt":"2025-05-15T13:12:16","slug":"sorensens-phosphate-buffer","status":"publish","type":"product","link":"https:\/\/www.em-grade.com\/de\/produkt\/sorensens-phosphate-buffer\/","title":{"rendered":"Sorensen&#8217;s Phosphate Buffer"},"content":{"rendered":"<p>Der Sorensen-Phosphatpuffer ist auch als Mischung von Natriumsalzen und Phosphat bekannt. Die Osmolarit\u00e4t einer 0,1 M L\u00f6sung bei pH 7,2 betr\u00e4gt 226 mosmols; durch Zugabe von 0,18 M Saccharose zur L\u00f6sung erh\u00f6ht sich die Osmolarit\u00e4t auf 425 mosmols. Der pH-Wert kann bei der Bestellung angegeben werden. Die maximale Lagerzeit nach Erhalt betr\u00e4gt 3 Monate.<\/p>\n<p><strong>Eigenschaften<\/strong><\/p>\n<ul>\n<li>Alle verwendeten Produkte werden aufgrund ihrer Reinheit ausgew\u00e4hlt.<\/li>\n<li>Lagerung: 4-8\u00b0C<\/li>\n<li>Verf\u00fcgbar in verschiedenen pH-Bereichen: 5,8 &#8211; 8<\/li>\n<\/ul>\n<p><strong>Vorteile und Nachteile<\/strong><\/p>\n<h4>\ud83d\udc4d Vorteile<\/h4>\n<ul>\n<li>Der physiologischste aller g\u00e4ngigen Puffer.<\/li>\n<li>Ahmt einige Komponenten der extrazellul\u00e4ren Fl\u00fcssigkeiten nach.<\/li>\n<li>Nicht toxisch f\u00fcr Zellen.<\/li>\n<li>pH-Wert variiert wenig mit der Temperatur.<\/li>\n<li>Stabil f\u00fcr mehrere Wochen bei 4\u00b0C.<\/li>\n<\/ul>\n<h4>\u26a0\ufe0f Nachteile<\/h4>\n<ul>\n<li>F\u00e4llung ist w\u00e4hrend der Fixierung wahrscheinlicher.<\/li>\n<li>Neigt dazu, F\u00e4llungen in Gegenwart von Calcium-Ionen zu bilden.<\/li>\n<li>F\u00e4llt Uranylacetat und neigt dazu, mit Bleisalzen zu reagieren.<\/li>\n<li>Kontaminiert langsam mit Mikroorganismen.<\/li>\n<\/ul>\n<p><strong>Herstellung von L\u00f6sungen<\/strong><\/p>\n<h3>Stamml\u00f6sungen<\/h3>\n<ul>\n<li>0,2 M Dibasisches Natriumphosphat 1 Liter:<\/li>\n<\/ul>\n<ul>\n<li>Na2HPO4 * 2H2O (MW = 178,05) 35,61 g<\/li>\n<li>oder Na2HPO4 * 7H2O (MW = 268,07) 53,65 g<\/li>\n<li>oder Na2HPO4 * 12H2O (MW = 358,14) 71,64 g<\/li>\n<li>ddH2O auf 1 Liter auff\u00fcllen<\/li>\n<\/ul>\n<ul>\n<li>0,2 M Monobasisches Natriumphosphat 1 Liter:<\/li>\n<\/ul>\n<ul>\n<li>NaH2PO4 * H2O (MW = 138,01) 27,6 g<\/li>\n<li>oder NaH2PO4 * 2H2O (MW = 156,03) 31,21 g<\/li>\n<li>ddH2O auf 1 Liter auff\u00fcllen<\/li>\n<\/ul>\n<h3>Arbeits-Puffer<\/h3>\n<ul>\n<li>0,1 M 100 ml: X ml 0,2 M dibasisches Natriumphosphat mit Y ml 0,2 M monobasisches Natriumphosphat mischen. Auf 100 ml mit ddH2O auff\u00fcllen oder 1:1 mit Fixativ verd\u00fcnnen.<\/li>\n<\/ul>\n<p><strong>pH-Tabelle<\/strong><\/p>\n<table style=\"height: 187px;\" width=\"364\">\n<thead>\n<tr>\n<th>pH (25\u00b0C)<\/th>\n<th>X ml<\/th>\n<th>Y ml<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>7,0<\/td>\n<td>30,5<\/td>\n<td>19,5<\/td>\n<\/tr>\n<tr>\n<td>7,2<\/td>\n<td>36,0<\/td>\n<td>14,0<\/td>\n<\/tr>\n<tr>\n<td>7,4<\/td>\n<td>40,5<\/td>\n<td>9,5<\/td>\n<\/tr>\n<tr>\n<td>7,6<\/td>\n<td>43,5<\/td>\n<td>6,5<\/td>\n<\/tr>\n<tr>\n<td>7,8<\/td>\n<td>45,75<\/td>\n<td>4,25<\/td>\n<\/tr>\n<tr>\n<td>8,0<\/td>\n<td>47,35<\/td>\n<td>2,65<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>Osmolarit\u00e4t<\/strong><\/p>\n<ul>\n<li>Die Osmolarit\u00e4t wird durch Variation der Molarit\u00e4t der Phosphate oder durch Zugabe von Saccharose, Glukose oder Natriumchlorid angepasst.<\/li>\n<li>pH 7,2:<\/li>\n<\/ul>\n<ul>\n<li>0,10 M = 226 mOs (Milliosmolen)<\/li>\n<li>0,05 M = 118 mOs<\/li>\n<li>0,075 M = 180 mOs<\/li>\n<li>0,15 M = 350 mOs<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<ul>\n<li>Verf\u00fcgbar von pH 6,9 bis 7,4<\/li>\n<li>Produktion auf Anfrage<\/li>\n<li>Mikrofiltrert 0,22\u00b5 &#8211; steril<\/li>\n<li>Unter neutralem Gas (Argon) versiegelt<\/li>\n<li>Hergestellt in Frankreich<\/li>\n<\/ul>\n","protected":false},"featured_media":6130,"template":"","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":""},"product_brand":[],"product_cat":[390],"product_tag":[],"class_list":["post-6128","product","type-product","status-publish","has-post-thumbnail","product_cat-puffer","pa_buffer-concentration-015m-de","pa_buffer-concentration-01m-de","pa_buffer-concentration-02m-de","pa_volume-1000ml-de","pa_volume-125ml-de","pa_volume-250ml-de","pa_volume-500ml-de","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/product\/6128","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/media\/6130"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/media?parent=6128"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/product_brand?post=6128"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/product_cat?post=6128"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/de\/wp-json\/wp\/v2\/product_tag?post=6128"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}