{"id":7161,"date":"2023-10-24T12:14:18","date_gmt":"2023-10-24T10:14:18","guid":{"rendered":"https:\/\/www.em-grade.com\/sin-categorizar\/tampones-en-microscopia\/"},"modified":"2024-06-12T11:31:18","modified_gmt":"2024-06-12T09:31:18","slug":"tampones-en-microscopia","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/es\/tampones\/tampones-en-microscopia\/","title":{"rendered":"Tampones en microscop\u00eda"},"content":{"rendered":"<p><a href=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-scaled.jpg\"><img alt=\"\" loading=\"lazy\" decoding=\"async\" class=\"wp-image-1860 alignleft\" src=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-150x150.jpg\" alt=\"\" width=\"234\" height=\"234\" srcset=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-150x150.jpg 150w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-140x140.jpg 140w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-100x100.jpg 100w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-500x500.jpg 500w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-350x350.jpg 350w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-1000x1000.jpg 1000w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-800x800.jpg 800w\" sizes=\"auto, (max-width: 234px) 100vw, 234px\" \/><\/a>La preparaci\u00f3n de las muestras para la observaci\u00f3n en microscopia electr\u00f3nica, requiere las mayores precauciones durante la utilizaci\u00f3n de varias soluciones seg\u00fan su protocolo en varias etapas, el respeto tambi\u00e9n de varios par\u00e1metros determinantes y muy importantes como:<\/p>\n\r\n<ul class=\"eplus-lPd1kt wp-block-list\">&#13;\n<li>el <strong>pH \/Buffer<\/strong>,<\/li>&#13;\n\r\n\r\n&#13;\n<li><strong>tipo de Buffer<\/strong> seg\u00fan la muestra,<\/li>&#13;\n\r\n\r\n&#13;\n<li><strong>Osmolaridad<\/strong> de la mezcla de enlace<\/li>&#13;\n\r\n\r\n&#13;\n<li><strong>temperatura<\/strong><\/li>&#13;\n\r\n\r\n&#13;\n<li><strong>duraci\u00f3n de la fijaci\u00f3n.<\/strong><\/li>&#13;\n<\/ul>\r\n<p>The pH of the different cell compartments can vary, so you need to adjust the pH of your buffer solutions according to your studies. In general, in more than 50% of the cases the buffers remain close from 7 to 7.4 A\u00a0<strong>buffer solution<\/strong>\u00a0maintains approximately the same\u00a0pH\u00a0despite the addition of small amounts of an\u00a0acid\u00a0or\u00a0base, or despite \u00a0dilution.<\/p>\r\n<ul>\r\n<li><strong><a href=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-scaled.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-1145 alignright\" src=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-150x150.jpg\" alt=\"PHEM Buffer\" width=\"272\" height=\"272\" srcset=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-150x150.jpg 150w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-140x140.jpg 140w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-100x100.jpg 100w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-500x500.jpg 500w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-350x350.jpg 350w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-1000x1000.jpg 1000w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-800x800.jpg 800w\" sizes=\"auto, (max-width: 272px) 100vw, 272px\" \/><\/a>phosphate or PB<\/strong>:\u00a0 A simple phosphate buffer is used ubiquitously in biological experiments, as it can be adapted to a variety of pH levels, including isotonic.\u00a0 This wide range is due to phosphoric acid having 3 dissociation constants<\/li>\r\n<li><strong>Phosphate Saline or PBS<\/strong> : PBS has many uses because it is isotonic and non-toxic to most cells. These uses include diluting the substance and flushing the cells. PBS with EDTA is also used to release attached and clustered cells. this buffer is used in cell culture under controlled atmosphere in incubators with 5% CO2   <\/li>\r\n<li><strong>D-PBS<\/strong>: <strong>el DPBS<\/strong> es <strong>Dulbecco&#8217;s PBS<\/strong> y tambi\u00e9n se conoce como CMF-PBS (libre de calcio-magnesio, o <strong>C<\/strong>a <strong>M<\/strong>g <strong>F<\/strong>ree). Ca Mg Free).<\/li>\r\n<li><strong>cacodilatos<\/strong> : El tamp\u00f3n cacodilato s\u00f3dico [Na (CH3) 2 AsO2 &#8211; 3H2O] es una alternativa al tamp\u00f3n fosfato de S\u00f8rensen. Tiene una buena capacidad amortiguadora del pH en el intervalo de pH 5,0-7,4. El cacodilato fue introducido para aplicaciones de microscop\u00eda electr\u00f3nica por Sabatini et al. (1962) como m\u00e9todo para evitar la adici\u00f3n de fosfatos adicionales a las preparaciones de muestras. Las mitocondrias y otros org\u00e1nulos pueden da\u00f1arse cuando se exponen a las altas concentraciones de fosfatos presentes en los tampones de S\u00f8rensen. Adem\u00e1s, el cacodilato no reacciona con los fijadores aldeh\u00eddicos como lo hacen los tampones que contienen aminas (por ejemplo, Tris). Su eficacia en soluciones fijadoras puede deberse al efecto inhibidor del metabolismo del arseniato m\u00e1s que a una capacidad tamp\u00f3n especial. Los tampones de Good, o reactivos formadores de tampones de Good, son una lista de 20 sustancias qu\u00edmicas formadoras de tampones seleccionadas por su idoneidad para el uso en bioqu\u00edmica por Norman Good y su equipo entre 1966 y 1980. Estos tampones se denominan \u00abtampones de Good\u00bb. Citar\u00e9 algunos de ellos utilizados en microscop\u00eda electr\u00f3nica.        <\/li>\r\n<\/ul>\r\n<ul>\r\n<li><strong>HEPES <\/strong><\/li>\r\n<li><strong>MOPS<\/strong><\/li>\r\n<li><strong>Tris , &#8230;<\/strong><\/li>\r\n<\/ul>\r\n","protected":false},"excerpt":{"rendered":"<p>La preparaci\u00f3n de las muestras para la observaci\u00f3n en microscopia electr\u00f3nica, requiere las mayores precauciones durante la utilizaci\u00f3n de varias soluciones seg\u00fan su protocolo en varias etapas, el respeto tambi\u00e9n&#8230;<\/p>\n","protected":false},"author":8,"featured_media":7164,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[452],"tags":[651],"class_list":["post-7161","post","type-post","status-publish","format-standard","has-post-thumbnail","category-tampones","tag-tampones"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts\/7161","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/comments?post=7161"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts\/7161\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media\/7164"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media?parent=7161"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/categories?post=7161"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/tags?post=7161"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}