{"id":7195,"date":"2023-10-24T13:56:17","date_gmt":"2023-10-24T11:56:17","guid":{"rendered":"https:\/\/www.em-grade.com\/sin-categorizar\/tampon-p-b-s\/"},"modified":"2024-03-14T14:21:22","modified_gmt":"2024-03-14T13:21:22","slug":"tampon-p-b-s","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/es\/tampones\/tampon-p-b-s\/","title":{"rendered":"Tamp\u00f3n P.B.S."},"content":{"rendered":"&#13;\n<p class=\"wp-block-paragraph\">La soluci\u00f3n <strong>salina tamponada con fosfato<\/strong> (<strong>PBS<\/strong>) es una soluci\u00f3n tamp\u00f3n utilizada habitualmente en <em>bioqu\u00edmica<\/em>. Es una soluci\u00f3n fisiol\u00f3gica con cloruro s\u00f3dico, fosfato dis\u00f3dico, fosfato monopot\u00e1sico y algo de cloruro pot\u00e1sico. En general, la concentraci\u00f3n de estas sales es la misma que en el cuerpo humano (isotonicidad).  <\/p>&#13;\n&#13;\n<p class=\"wp-block-paragraph\">Este tamp\u00f3n se utiliza principalmente para enjuagar las c\u00e9lulas cuando es necesario eliminar todos los restos de medio antes de procesarlas. Est\u00e1 pensado para su uso en c\u00e9lulas mantenidas fuera del incubador de CO<sub>2<\/sub> y no est\u00e1 dise\u00f1ado para incubaciones a largo plazo. Su capacidad tamp\u00f3n se basa en el par dihidr\u00f3geno fosfato\/hidr\u00f3geno fosfato (pK<sub>A<\/sub>= 7,2), que es tambi\u00e9n uno de los tres mecanismos principales de mantenimiento del pH sangu\u00edneo (HPO<sub>4<\/sub><sup>2-<\/sup> + H<sup>+<\/sup> = H<sub>2<\/sub>PO<sub>4<\/sub><sup>&#8211;<\/sup>).  <\/p>&#13;\n","protected":false},"excerpt":{"rendered":"<p>&#13; La soluci\u00f3n salina tamponada con fosfato (PBS) es una soluci\u00f3n tamp\u00f3n utilizada habitualmente en bioqu\u00edmica. Es una soluci\u00f3n fisiol\u00f3gica con cloruro s\u00f3dico, fosfato dis\u00f3dico, fosfato monopot\u00e1sico y algo de&#8230;<\/p>\n","protected":false},"author":8,"featured_media":7798,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[452],"tags":[657],"class_list":["post-7195","post","type-post","status-publish","format-standard","has-post-thumbnail","category-tampones","tag-tampon-p-b-s"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts\/7195","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/comments?post=7195"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts\/7195\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media\/7798"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media?parent=7195"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/categories?post=7195"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/tags?post=7195"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}