{"id":7255,"date":"2023-11-10T11:14:41","date_gmt":"2023-11-10T10:14:41","guid":{"rendered":"https:\/\/www.em-grade.com\/sin-categorizar\/el-tampon-imidazol-permite-la-retencion-de-lipidos\/"},"modified":"2023-11-10T11:14:41","modified_gmt":"2023-11-10T10:14:41","slug":"el-tampon-imidazol-permite-la-retencion-de-lipidos","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/es\/tampones\/el-tampon-imidazol-permite-la-retencion-de-lipidos\/","title":{"rendered":"El tamp\u00f3n imidazol permite la retenci\u00f3n de l\u00edpidos"},"content":{"rendered":"

El tamp\u00f3n imidazol permite conservar los l\u00edpidos intracelulares m\u00e1s dif\u00edciles de preservar<\/strong>.<\/p>\n

El tamp\u00f3n imidazol permite la retenci\u00f3n de l\u00edpidos.<\/u><\/strong> asociado al osmio durante la fijaci\u00f3n como tamp\u00f3n a 0,1M<\/p>\n

he aqu\u00ed un protocolo est\u00e1ndar<\/p>\n

    \n
  1. Glutaraldeh\u00eddo ‘2 a 2,5% en tamp\u00f3n imidazol 0,1M<\/strong> pH7,5 – RT<\/li>\n
  2. Lavado en tamp\u00f3n imidazol 0,2M<\/strong> pH7,5 (2 ba\u00f1os 3 min) en RT<\/li>\n
  3. Osmio 1% a 2% en tamp\u00f3n imidazol 0,1<\/strong> pH 7,5, 30 min – RT<\/li>\n
  4. \u00daltimo lavado igual que el punto 2- (Lavado en tamp\u00f3n imidazol 0.2M pH7.5 (2 ba\u00f1os no m\u00e1s, 3 mins) en RT<\/li>\n<\/ol>\n

    Sustituya sus tampones Sorensen PBS o cacodilato habituales por imidazol , cuando su estudio d\u00e9 importancia a las inclusiones lip\u00eddicas.<\/p>\n

    nota importante: si se quiere preservar el contenido de las c\u00e9lulas fijadas, evitar lavar demasiado, 1 lavado es suficiente, si los l\u00edpidos simples son los m\u00e1s dif\u00edciles de preservar, incluso se debe evitar el lavado.<\/p>\n

    \"\" <\/a> \"tamp\u00f3n<\/a><\/p>\n

    c\u00e9lula hepatocitaria<\/p>\n","protected":false},"excerpt":{"rendered":"

    El tamp\u00f3n imidazol permite conservar los l\u00edpidos intracelulares m\u00e1s dif\u00edciles de preservar. El tamp\u00f3n imidazol permite la retenci\u00f3n de l\u00edpidos. asociado al osmio durante la fijaci\u00f3n como tamp\u00f3n a 0,1M…<\/p>\n","protected":false},"author":8,"featured_media":7257,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[452],"tags":[678,677],"class_list":["post-7255","post","type-post","status-publish","format-standard","has-post-thumbnail","category-tampones","tag-lipidos-es","tag-tampon-imidazol"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts\/7255","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/comments?post=7255"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts\/7255\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media\/7257"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media?parent=7255"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/categories?post=7255"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/tags?post=7255"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}