{"id":7335,"date":"2023-11-17T11:33:02","date_gmt":"2023-11-17T10:33:02","guid":{"rendered":"https:\/\/www.em-grade.com\/sin-categorizar\/em-stain-ote-oolong-tea-uso-tincion-seccion-ultrafina\/"},"modified":"2024-03-08T11:33:52","modified_gmt":"2024-03-08T10:33:52","slug":"em-stain-ote-oolong-tea-uso-tincion-seccion-ultrafina","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/es\/mancha-em-es\/em-stain-ote-oolong-tea-uso-tincion-seccion-ultrafina\/","title":{"rendered":"EM Stain OTE (OOLONG TEA) uso tinci\u00f3n secci\u00f3n ultrafina."},"content":{"rendered":"

Preparaci\u00f3n y utilizaci\u00f3n de la O.T.E.<\/p>\n

La preparaci\u00f3n de la soluci\u00f3n OTE es similar a la infusi\u00f3n de agua de t\u00e9 70\u00b0C o Buffer 0,1M.(*)<\/p>\n

– Con un agitador magn\u00e9tico calefactor a 70\u00b0C, disolver el polvo de OTE a una concentraci\u00f3n del 0,2% (ya que la saturaci\u00f3n se alcanza al 0,3%).<\/p>\n

– Despu\u00e9s de la disoluci\u00f3n completa (15 a 30 minutos con agitaci\u00f3n a 70 \u00b0 C),<\/p>\n

    \n
  1. Filtre su soluci\u00f3n de extracto de OTE a 0,45\u00b5.<\/li>\n
  2. Almacenar en un peque\u00f1o tubo Eppendorf lleno al m\u00e1ximo para reducir el aire que contiene ox\u00edgeno o en una botella AirLess.<\/li>\n
  3. Conservar en la oscuridad a 4 \u00b0C (cuando se manipulen, dejar siempre que las soluciones vuelvan a RT)<\/li>\n
  4. Contraste sus rejillas con OTE durante 15 a 25 minutos, oscuridad, RT<\/li>\n
  5. Escurrir sin enjuagar<\/li>\n
  6. Post-tinci\u00f3n 1 a 2 minutos con citrato de plomo seg\u00fan Reynolds (Ref # 11300 de Delta Microscopies)<\/li>\n
  7. Escurrir, enjuagar brevemente, secar y listo para ver.<\/li>\n<\/ol>\n

    *nota: la soluci\u00f3n puede conservarse durante no m\u00e1s de una semana, pero m\u00e1s tiempo en una soluci\u00f3n tamp\u00f3n de cacodilato, botella AirLess (sin crecimiento bacteriano).<\/p>\n

    \"\" <\/a>\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

    Preparaci\u00f3n y utilizaci\u00f3n de la O.T.E. La preparaci\u00f3n de la soluci\u00f3n OTE es similar a la infusi\u00f3n de agua de t\u00e9 70\u00b0C o Buffer 0,1M.(*) – Con un agitador magn\u00e9tico…<\/p>\n","protected":false},"author":8,"featured_media":7273,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[669],"tags":[692,691],"class_list":["post-7335","post","type-post","status-publish","format-standard","has-post-thumbnail","category-mancha-em-es","tag-extracto-de-te-oolong","tag-ote-es"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts\/7335","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/comments?post=7335"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts\/7335\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media\/7273"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media?parent=7335"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/categories?post=7335"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/tags?post=7335"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}