{"id":7341,"date":"2023-11-20T15:36:13","date_gmt":"2023-11-20T14:36:13","guid":{"rendered":"https:\/\/www.em-grade.com\/sin-categorizar\/phem-buffer-el-mejor-tampon-para-el-cultivo-celular-de-inmunofluorescencia\/"},"modified":"2023-11-20T15:36:13","modified_gmt":"2023-11-20T14:36:13","slug":"phem-buffer-el-mejor-tampon-para-el-cultivo-celular-de-inmunofluorescencia","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/es\/tampones\/phem-buffer-el-mejor-tampon-para-el-cultivo-celular-de-inmunofluorescencia\/","title":{"rendered":"PHEM BUFFER , el mejor tamp\u00f3n para el cultivo celular de inmunofluorescencia."},"content":{"rendered":"

El tamp\u00f3n PHEM<\/strong> se desarroll\u00f3 para preservar los microt\u00fabulos y se utiliza para preservar las c\u00e9lulas<\/span> <\/span>estructura en inmunotinci\u00f3n<\/span><\/p>\n

Referencias<\/strong> : Schliwa, Manfred, y Jonathan Van Blerkom. \u00abStructural interaction of cytoskeletal components.\u00bb The Journal of Cell Biology 90.1 (1981): 222-235. <\/span><\/em><\/p>\n

El tamp\u00f3n PHEM se recomienda en estudios inmunocitoqu\u00edmicos y de inmunotinci\u00f3n de tejidos y cultivos celulares.<\/p>\n

\"\" <\/a>\"\"<\/a><\/p>\n

Los trabajos de laboratorio han demostrado que el tamp\u00f3n PHEM permite una mejor detecci\u00f3n de los ant\u00edgenos intracelulares y un menor impacto en las reacciones enzim\u00e1ticas y bioqu\u00edmicas, mientras que el PBS es m\u00e1s espec\u00edfico para los ant\u00edgenos extracelulares de membrana, por esta raz\u00f3n se prefiere el PHEM durante los marcajes fluorescentes;<\/p>\n

El uso de PHEM tambi\u00e9n garantiza una excelente conservaci\u00f3n de la ultraestructura celular, en particular de los microt\u00fabulos. El tamp\u00f3n conocido como CSK, un derivado de la formulaci\u00f3n PHEM se llama C<\/strong>ytoSK<\/strong>eletal (CSK) Buffer parece mejorar el etiquetado de microt\u00fabulos. <\/p>\n

 <\/p>\n

m\u00e1s informaci\u00f3n<\/strong><\/span> PHEM buffer Receta<\/a><\/h3>\n","protected":false},"excerpt":{"rendered":"

El tamp\u00f3n PHEM se desarroll\u00f3 para preservar los microt\u00fabulos y se utiliza para preservar las c\u00e9lulas estructura en inmunotinci\u00f3n Referencias : Schliwa, Manfred, y Jonathan Van Blerkom. \u00abStructural interaction of…<\/p>\n","protected":false},"author":8,"featured_media":7278,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[452],"tags":[699,700],"class_list":["post-7341","post","type-post","status-publish","format-standard","has-post-thumbnail","category-tampones","tag-em-stain-es","tag-phem-es"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts\/7341","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/comments?post=7341"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts\/7341\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media\/7278"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media?parent=7341"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/categories?post=7341"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/tags?post=7341"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}