{"id":7349,"date":"2023-11-23T11:42:59","date_gmt":"2023-11-23T10:42:59","guid":{"rendered":"https:\/\/www.em-grade.com\/sin-categorizar\/como-preparar-la-solucion-de-tintura-o-t-e-oolong\/"},"modified":"2023-11-23T11:42:59","modified_gmt":"2023-11-23T10:42:59","slug":"como-preparar-la-solucion-de-tintura-o-t-e-oolong","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/es\/mancha-em-es\/como-preparar-la-solucion-de-tintura-o-t-e-oolong\/","title":{"rendered":"\u00bfC\u00f3mo preparar la soluci\u00f3n de tintura O.T.E (Oolong)?"},"content":{"rendered":"<p><iframe loading=\"lazy\" title=\"OTE EM staining ( Oolong Tea Extract ) . EM Stain\" width=\"1080\" height=\"608\" src=\"https:\/\/www.youtube.com\/embed\/i7lZhu4Pf9o?start=104&#038;feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" allowfullscreen><\/iframe><\/p>\n<h3><strong><span style=\"color: #000000;\">Protocole<\/span><\/strong><\/h3>\n<p>La preparaci\u00f3n de la soluci\u00f3n OTE es similar a la infusi\u00f3n de agua de t\u00e9 70 \u00b0 C no m\u00e1s.<\/p>\n<p>&#8211; Con un agitador magn\u00e9tico a 70\u00b0C, disolver el polvo de OTE a una concentraci\u00f3n del 0,2% como m\u00e1ximo (ya que la saturaci\u00f3n se alcanza al 0,3%).<\/p>\n<p>&#8211; Despu\u00e9s de la disoluci\u00f3n completa (alcanzado en 15 a 30 minutos con agitaci\u00f3n a 70 \u00b0 C),<\/p>\n<ol>\n<li>Filtre su soluci\u00f3n de extracto de OTE a 0,45\u00b5 o mejor a 0,22\u00b5<\/li>\n<li>Almacenar en un peque\u00f1o tubo Eppendorf lleno al m\u00e1ximo para reducir el aire que contiene ox\u00edgeno o en un <a href=\"https:\/\/www.em-grade.com\/microscopy-advice\/bottles-with-airless-system\/\">vial AirLess<\/a>.<\/li>\n<li>Conservar en la oscuridad a 4 \u00b0C (al manipular, dejar siempre que las soluciones vuelvan a RT).<\/li>\n<li>Contraste sus rejillas con OTE durante 15 a 20 minutos, oscuridad, RT<\/li>\n<li>Escurrir simplemente no enjuague o lavado muy corto 1 a 2 segundos, despu\u00e9s del secado<\/li>\n<li>Post-mancha 1 a 2 minutos con citrato de plomo seg\u00fan Reynolds (Ref # 11300)<\/li>\n<li>Escurrir, enjuagar brevemente, secar y listo para observar.<\/li>\n<\/ol>\n<p><b>   <\/b><\/p>\n<p>Polvo OTE , 10gr s\u00f3lo por <a href=\"https:\/\/www.em-grade.com\/product\/oolong-tea-extract-ote-powder\/\"><strong>EM-grade.com<\/strong><\/a><\/p>\n<p><a href=\"https:\/\/www.em-grade.com\/product\/oolong-tea-extract-ote-powder\/\"><span style=\"color: #ff0000;\"><b>C\u00f3digo producto # GP18-10<\/b><\/span><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Protocole La preparaci\u00f3n de la soluci\u00f3n OTE es similar a la infusi\u00f3n de agua de t\u00e9 70 \u00b0 C no m\u00e1s. &#8211; Con un agitador magn\u00e9tico a 70\u00b0C, disolver el&#8230;<\/p>\n","protected":false},"author":8,"featured_media":7350,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[669],"tags":[704],"class_list":["post-7349","post","type-post","status-publish","format-standard","has-post-thumbnail","category-mancha-em-es","tag-o-t-e-es"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts\/7349","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/comments?post=7349"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts\/7349\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media\/7350"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media?parent=7349"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/categories?post=7349"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/tags?post=7349"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}