{"id":7367,"date":"2024-01-19T17:03:56","date_gmt":"2024-01-19T16:03:56","guid":{"rendered":"https:\/\/www.em-grade.com\/sin-categorizar\/receta-de-tampon-phem\/"},"modified":"2024-01-19T17:03:56","modified_gmt":"2024-01-19T16:03:56","slug":"receta-de-tampon-phem","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/es\/tampones\/receta-de-tampon-phem\/","title":{"rendered":"Receta de tamp\u00f3n PHEM"},"content":{"rendered":"

Receta de tamp\u00f3n PHEM<\/em><\/h2>\n

El tamp\u00f3n PHEM se ha utilizado principalmente para tejidos y cultivos celulares que se procesan para estudios inmunocitoqu\u00edmicos. La mayor\u00eda de los ant\u00edgenos, especialmente los intracelulares, se ti\u00f1en mejor utilizando PHEM que los procesados con PBS, tambi\u00e9n tiene un efecto m\u00e1s limitado sobre las reacciones bioqu\u00edmicas y las enzimas. La muy buena conservaci\u00f3n de la ultraestructura celular, especialmente de los microt\u00fabulos, es tambi\u00e9n una ventaja del uso de PHEM.<\/p>\n

\"\"<\/a><\/strong><\/p>\n

Referencias:<\/strong><\/p>\n

    \n
  1. Schliwa, Manfred, and Jonathan Van Blerkom<\/strong><\/em>. \u00abStructural interaction of cytoskeletal components.\u00bb The Journal of Cell Biology 90.1 (1981): 222-235.<\/li>\n
  2. Jacqueline Montanaro<\/em><\/strong>: Mejora de la ultraestructura de invertebrados marinos utilizando tampones no t\u00f3xicos<\/li>\n<\/ol>\n

    <\/h4>\n

    Receta de preparaci\u00f3n para 500 ml de tamp\u00f3n PHEM a 0,2M <\/u><\/strong><\/span><\/h4>\n

    Disolver en 400 ml de agua destilada estos reactivos en polvo en el siguiente orden:<\/em><\/p>\n\n\n\n\n\n\n\n\n
    Polvo reactivo<\/strong><\/td>\nNumero C.A.S<\/strong><\/td>\npeso gramo<\/strong><\/td>\nRef. em-grade<\/strong><\/td>\n<\/tr>\n
    HEPES<\/strong><\/td>\n7365-45-9<\/td>\n0.598 gr.<\/td>\nGP19<\/a><\/strong><\/td>\n<\/tr>\n
    EGTA<\/strong><\/td>\n67-42-5<\/td>\n0.380 gr.<\/td>\nGP27<\/strong><\/td>\n<\/tr>\n
    MgSO4 , 7H2O<\/strong><\/td>\n10034-99-8<\/td>\n0.098 gr<\/td>\nGP28<\/strong><\/td>\n<\/tr>\n
    P.I.P.E.S.<\/strong><\/td>\n5625-37-6<\/td>\n1.814 gr<\/td>\nGP21<\/a><\/strong><\/td>\n<\/tr>\n
    WATER D<\/em>.<\/td>\nEnrasar a 500 ml de volumen final y ajustar al pH deseado con NaOH.<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    Filtrar la soluci\u00f3n a 0,22\u00b5.<\/em><\/p>\n

    Tambi\u00e9n ofrecemos nuestro tamp\u00f3n PHEM listo para usar disponible en nuestra tienda: Tamp\u00f3n PHEM<\/a>. <\/span><\/h4>\n

    Referencia : GT140000<\/span><\/a><\/span><\/span><\/h4>\n","protected":false},"excerpt":{"rendered":"

    Receta de tamp\u00f3n PHEM El tamp\u00f3n PHEM se ha utilizado principalmente para tejidos y cultivos celulares que se procesan para estudios inmunocitoqu\u00edmicos. La mayor\u00eda de los ant\u00edgenos, especialmente los intracelulares,…<\/p>\n","protected":false},"author":8,"featured_media":7281,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[452],"tags":[710],"class_list":["post-7367","post","type-post","status-publish","format-standard","has-post-thumbnail","category-tampones","tag-tampon-phem-es"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts\/7367","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/comments?post=7367"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/posts\/7367\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media\/7281"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media?parent=7367"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/categories?post=7367"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/tags?post=7367"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}