{"id":5051,"date":"2023-12-04T16:35:50","date_gmt":"2023-12-04T15:35:50","guid":{"rendered":"https:\/\/www.em-grade.com\/product\/paraformaldehyde-pfa-2-4-glutaraldehyde-2-25-in-01m-buffer-karnosky-buffer\/"},"modified":"2026-07-16T18:55:54","modified_gmt":"2026-07-16T16:55:54","slug":"paraformaldehyde-pfa-2-4-glutaraldehyde-2-25-in-01m-buffer-karnosky-buffer","status":"publish","type":"product","link":"https:\/\/www.em-grade.com\/es\/producto\/paraformaldehyde-pfa-2-4-glutaraldehyde-2-25-in-01m-buffer-karnosky-buffer\/","title":{"rendered":"Paraformaldehyde (PFA) (2% – 4%), Glutaraldehyde (2% – 2,5%) in 0,1M Buffer – KARNOSKY in buffer"},"content":{"rendered":"
\n

Description du produit:<\/span><\/span><\/strong><\/p>\n<\/div>\n

Le fixateur de Karnovsky, d\u00e9velopp\u00e9 par Morris J. Karnovsky, est un fixateur r\u00e9put\u00e9 utilis\u00e9 principalement en microscopie \u00e9lectronique. Son but est de pr\u00e9server m\u00e9ticuleusement les structures cellulaires, permettant ainsi un examen d\u00e9taill\u00e9 au microscope \u00e9lectronique.<\/span><\/span><\/p>\n

Application <\/span><\/span><\/strong>:<\/span><\/span><\/strong><\/h4>\n

Ce fixateur est essentiel pour les chercheurs et les pathologistes qui ont besoin d’une visualisation d\u00e9taill\u00e9e des composants cellulaires au niveau microscopique. Il est particuli\u00e8rement pr\u00e9cieux dans l’\u00e9tude d’\u00e9chantillons biologiques complexes, notamment l’ultrastructure des fragments du bassinet du rein, gr\u00e2ce \u00e0 sa capacit\u00e9 \u00e0 maintenir l’int\u00e9grit\u00e9 et la morphologie des structures cellulaires.<\/span><\/span><\/p>\n

Pr\u00e9paration de la solution m\u00e8re de fixateur de Karnovsky :<\/span><\/span><\/p>\n

    \n
  1. Paraformald\u00e9hyde :<\/span><\/span><\/strong> Commencez avec 2,0 g de paraformald\u00e9hyde.<\/span><\/span><\/li>\n
  2. Eau distill\u00e9e :<\/span><\/span><\/strong> Ajouter 25 ml d\u2019eau distill\u00e9e.<\/span><\/span><\/li>\n
  3. Hydroxyde de sodium :<\/span><\/span><\/strong> Introduisez 2 \u00e0 4 gouttes d\u2019hydroxyde de sodium 1 M dans le m\u00e9lange pour dissoudre le paraformald\u00e9hyde.<\/span><\/span><\/li>\n
  4. Glutarald\u00e9hyde :<\/span><\/span><\/strong> incorporer 5,0 ml de glutarald\u00e9hyde \u00e0 50 % pour r\u00e9ticuler les prot\u00e9ines.<\/span><\/span><\/li>\n
  5. Tampon cacodylate :<\/span><\/span><\/strong> Enfin, ajoutez 20,0 ml de tampon cacodylate 0,2 M \u00e0 pH 7,4 pour stabiliser le pH.<\/span><\/span><\/li>\n<\/ol>\n

    Conseils :\u00a0<\/span><\/span><\/strong><\/h4>\n

    Le processus de pr\u00e9paration consiste \u00e0 dissoudre initialement le paraformald\u00e9hyde dans de l’eau distill\u00e9e, puis \u00e0 ajouter de l’hydroxyde de sodium. Ensuite, du glutarald\u00e9hyde et un tampon cacodylate sont m\u00e9lang\u00e9s pour compl\u00e9ter la solution. Des ajustements peuvent \u00eatre apport\u00e9s \u00e0 cette formule pour r\u00e9pondre \u00e0 des besoins de recherche sp\u00e9cifiques, ce qui permet une application polyvalente dans diverses \u00e9tudes.<\/span><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

      \n
    • fixateur karnovsky, tamponn\u00e9 pour application imm\u00e9diate<\/span><\/span><\/li>\n
    • solution pr\u00e9-m\u00e9lang\u00e9e et pr\u00eate \u00e0 l’emploi<\/span><\/span><\/li>\n
    • formul\u00e9 avec de l’eau distill\u00e9e pour plus de puret\u00e9<\/span><\/span><\/li>\n
    • qualit\u00e9 de microscopie \u00e9lectronique (EM)<\/span><\/span><\/li>\n<\/ul>\n","protected":false},"featured_media":6737,"template":"","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":""},"product_brand":[],"product_cat":[415,417],"product_tag":[],"class_list":["post-5051","product","type-product","status-publish","has-post-thumbnail","product_cat-fijador","product_cat-fijadores-especificos","pa_buffer-concentration-01m-es","pa_buffer-concentration-02m-es","pa_buffer-type-cacodylate-buffer-es","pa_buffer-type-phosphate-buffer-es","pa_glutaraldehyde-concentration-25-es","pa_glutaraldehyde-concentration-2-es","pa_glutaraldehyde-concentration-3-es","pa_paraformaldehyde-concentrati-25-es","pa_paraformaldehyde-concentrati-2-es","pa_paraformaldehyde-concentrati-4-es","pa_volume-10ml-x-10-es","pa_volume-125ml-es","pa_volume-30ml-es","pa_volume-500ml-es","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product\/5051","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media\/6737"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media?parent=5051"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product_brand?post=5051"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product_cat?post=5051"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product_tag?post=5051"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}