{"id":6162,"date":"2023-12-04T16:35:00","date_gmt":"2023-12-04T15:35:00","guid":{"rendered":"https:\/\/www.em-grade.com\/product\/sorensens-phosphate-buffer\/"},"modified":"2025-05-15T15:19:27","modified_gmt":"2025-05-15T13:19:27","slug":"sorensens-phosphate-buffer","status":"publish","type":"product","link":"https:\/\/www.em-grade.com\/es\/producto\/sorensens-phosphate-buffer\/","title":{"rendered":"Sorensen&#8217;s Phosphate Buffer"},"content":{"rendered":"<p>El tamp\u00f3n fosfato de Sorensen tambi\u00e9n se conoce como una mezcla de sales de sodio y fosfato. La osmolaridad de una soluci\u00f3n 0,1 M a pH 7,2 es de 226 mosmoles; la adici\u00f3n de 0,18 M de sacarosa a la soluci\u00f3n aumenta la osmolaridad a 425 mosmoles. El pH puede ser especificado en el momento del pedido. La duraci\u00f3n m\u00e1xima de almacenamiento despu\u00e9s de la recepci\u00f3n es de 3 meses.<\/p>\n<p><strong>Caracter\u00edsticas<\/strong><\/p>\n<p>Todos los productos utilizados son seleccionados por su pureza. Almacenamiento: 4-8\u00b0C Disponible en diferentes intervalos de pH: 5,8 \u2013 8<\/p>\n<p><strong>Ventajas y Desventajas<\/strong><\/p>\n<p>\ud83d\udc4d Ventajas<\/p>\n<ul>\n<li>El m\u00e1s fisiol\u00f3gico de todos los tampones comunes.<\/li>\n<li>Imita algunos componentes de los fluidos extracelulares.<\/li>\n<li>No t\u00f3xico para las c\u00e9lulas.<\/li>\n<li>El pH var\u00eda poco con la temperatura.<\/li>\n<li>Estable durante varias semanas a 4\u00b0C.<\/li>\n<\/ul>\n<p>\u26a0\ufe0f Desventajas<\/p>\n<ul>\n<li>La precipitaci\u00f3n es m\u00e1s probable durante la fijaci\u00f3n.<\/li>\n<li>Tiende a formar precipitados en presencia de iones calcio.<\/li>\n<li>Precipita el acetato de uranilo y tiende a reaccionar con los sales de plomo.<\/li>\n<li>Se contamina lentamente con microorganismos.<\/li>\n<\/ul>\n<p><strong>Preparaci\u00f3n de las Soluciones<\/strong><\/p>\n<p><strong>Soluciones Madre<\/strong><\/p>\n<ul>\n<li>0,2 M Fosfato de Sodio Dib\u00e1sico 1 litro: Na2HPO4 * 2H2O (PM = 178,05) 35,61 g o Na2HPO4 * 7H2O (PM = 268,07) 53,65 g o Na2HPO4 * 12H2O (PM = 358,14) 71,64 g ddH2O para llevar a 1 litro<\/li>\n<li>0,2 M Fosfato de Sodio Monob\u00e1sico 1 litro: NaH2PO4 * H2O (PM = 138,01) 27,6 g o NaH2PO4 * 2H2O (PM = 156,03) 31,21 g ddH2O para llevar a 1 litro<\/li>\n<\/ul>\n<p><strong>Tamp\u00f3n de Trabajo<\/strong><\/p>\n<ul>\n<li>0,1 M 100 ml: Mezclar X ml de fosfato de sodio dib\u00e1sico 0,2 M con Y ml de fosfato de sodio monob\u00e1sico 0,2 M. Diluir a 100 ml con ddH2O o diluir 1:1 con fijador.<\/li>\n<\/ul>\n<p><strong>Tabla de pH<\/strong><\/p>\n<table style=\"height: 193px;\" width=\"363\">\n<thead>\n<tr>\n<th>pH (25\u00b0C)<\/th>\n<th>X ml<\/th>\n<th>Y ml<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>7,0<\/td>\n<td>30,5<\/td>\n<td>19,5<\/td>\n<\/tr>\n<tr>\n<td>7,2<\/td>\n<td>36,0<\/td>\n<td>14,0<\/td>\n<\/tr>\n<tr>\n<td>7,4<\/td>\n<td>40,5<\/td>\n<td>9,5<\/td>\n<\/tr>\n<tr>\n<td>7,6<\/td>\n<td>43,5<\/td>\n<td>6,5<\/td>\n<\/tr>\n<tr>\n<td>7,8<\/td>\n<td>45,75<\/td>\n<td>4,25<\/td>\n<\/tr>\n<tr>\n<td>8,0<\/td>\n<td>47,35<\/td>\n<td>2,65<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>Osmolaridad<\/strong><\/p>\n<p>La osmolaridad se regula variando la molaridad de los fosfatos o agregando sacarosa, glucosa o cloruro de sodio. pH 7,2:<\/p>\n<ul>\n<li>0,10 M = 226 mOs (mili osmoles)<\/li>\n<li>0,05 M = 118 mOs<\/li>\n<li>0,075 M = 180 mOs<\/li>\n<li>0,15 M = 350 mOs<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<ul>\n<li>Disponible desde pH 6,9 hasta 7,4<\/li>\n<li>Producci\u00f3n bajo demanda<\/li>\n<li>Microfiltrado 0,22\u00b5 &#8211; est\u00e9ril<\/li>\n<li>Sellado bajo gas neutro (Arg\u00f3n)<\/li>\n<li>Fabricado en Francia<\/li>\n<\/ul>\n","protected":false},"featured_media":6164,"template":"","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":""},"product_brand":[],"product_cat":[414],"product_tag":[],"class_list":["post-6162","product","type-product","status-publish","has-post-thumbnail","product_cat-tampon","pa_buffer-concentration-015m-es","pa_buffer-concentration-01m-es","pa_buffer-concentration-02m-es","pa_volume-1000ml-es","pa_volume-125ml-es","pa_volume-250ml-es","pa_volume-500ml-es","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product\/6162","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media\/6164"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media?parent=6162"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product_brand?post=6162"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product_cat?post=6162"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product_tag?post=6162"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}