{"id":6217,"date":"2023-12-04T16:34:56","date_gmt":"2023-12-04T15:34:56","guid":{"rendered":"https:\/\/www.em-grade.com\/product\/phosphate-buffered-saline-pbs\/"},"modified":"2024-05-17T15:00:54","modified_gmt":"2024-05-17T13:00:54","slug":"phosphate-buffered-saline-pbs","status":"publish","type":"product","link":"https:\/\/www.em-grade.com\/es\/producto\/phosphate-buffered-saline-pbs\/","title":{"rendered":"Phosphate Buffered Saline (PBS)"},"content":{"rendered":"<h4><strong>Product description:<\/strong><\/h4>\n<p><a href=\"https:\/\/www.em-grade.com\/es\/tampones\/tampon-p-b-s\/\">Phosphate-buffered<\/a> saline (often abbreviated as PBS) is a commonly used buffer solution in biochemistry. It is a physiological solution containing sodium chloride, disodium phosphate, monopotassium phosphate, and a small amount of potassium chloride. Generally, the concentration of these salts is equivalent to that of the human body (isotonicity).<\/p>\n<h4><strong>Application:<\/strong><\/h4>\n<p>This buffer is primarily used for rinsing cells to remove any traces of medium before treatment. It is intended for use on cells outside of the CO2 incubator and is not designed for long-term incubations.<\/p>\n<p>The composition of 1X PBS is:<\/p>\n<ul>\n<li>8.0 g\/L NaCl,<\/li>\n<li>0.2 g\/L KCl,<\/li>\n<li>1.42 g\/L Na2HPO4,<\/li>\n<li>0.24 g\/L KH2PO4 for 1000 ml of distilled water.<\/li>\n<\/ul>\n<h4><strong>Tip:<\/strong><\/h4>\n<p><a href=\"https:\/\/www.em-grade.com\/es\/tampones\/tampones-en-microscopia\/\">A\u00a0<strong>buffer solution<\/strong>\u00a0maintains approximately the same\u00a0pH\u00a0despite the addition of small amounts of an\u00a0acid\u00a0or\u00a0base, or despite \u00a0dilution.<\/a><\/p>\n<p>Its buffering capacity relies on the dihydrogen phosphate\/hydrogen phosphate pair (pKa = 7.2), which is also one of the three major mechanisms for maintaining blood pH (HPO4^2- + H+ = H2PO4^-).<br \/>\nStorage: RT, Note: It crystallizes at low temperatures.<\/p>\n","protected":false},"excerpt":{"rendered":"<div class=\"flex-1 overflow-hidden\">\n<div class=\"react-scroll-to-bottom--css-phoan-79elbk h-full\">\n<div class=\"react-scroll-to-bottom--css-phoan-1n7m0yu\">\n<div class=\"flex flex-col text-sm pb-9\">\n<div class=\"w-full text-token-text-primary\" data-testid=\"conversation-turn-38\">\n<div class=\"px-4 py-2 justify-center text-base md:gap-6 m-auto\">\n<div class=\"flex flex-1 text-base mx-auto gap-3 md:px-5 lg:px-1 xl:px-5 md:max-w-3xl lg:max-w-[40rem] xl:max-w-[48rem] group final-completion\">\n<div class=\"relative flex w-full flex-col agent-turn\">\n<div class=\"flex-col gap-1 md:gap-3\">\n<div class=\"flex flex-grow flex-col max-w-full\">\n<div class=\"min-h-[20px] text-message flex flex-col items-start gap-3 whitespace-pre-wrap break-words [.text-message+&amp;]:mt-5 overflow-x-auto\" data-message-author-role=\"assistant\" data-message-id=\"d4c6bb38-ee66-4b54-a2ab-6a4f6749a27e\">\n<div class=\"markdown prose w-full break-words dark:prose-invert light\">\n<ul>\n<li>available from pH 6.9 to 7.4<\/li>\n<li>production on demand<\/li>\n<li>microfiltered 0.22\u00b5 \u2013 st\u00e9rile<\/li>\n<li>sealed under neutral gas (Argon)<\/li>\n<li>made in France<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"featured_media":11053,"template":"","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":""},"product_brand":[],"product_cat":[414],"product_tag":[],"class_list":["post-6217","product","type-product","status-publish","has-post-thumbnail","product_cat-tampon","pa_buffer-concentration-015x-es","pa_buffer-concentration-10x-es","pa_buffer-concentration-1x-es","pa_buffer-concentration-2x-es","pa_buffer-concentration-5x-es","pa_volume-1000ml-es","pa_volume-125ml-es","pa_volume-250ml-es","pa_volume-500ml-es","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product\/6217","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media\/11053"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/media?parent=6217"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product_brand?post=6217"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product_cat?post=6217"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/es\/wp-json\/wp\/v2\/product_tag?post=6217"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}