{"id":6507,"date":"2023-12-04T16:34:47","date_gmt":"2023-12-04T15:34:47","guid":{"rendered":"https:\/\/www.em-grade.com\/product\/stain77-emstain-solution\/"},"modified":"2024-03-06T14:54:49","modified_gmt":"2024-03-06T13:54:49","slug":"stain77-emstain-solution","status":"publish","type":"product","link":"https:\/\/www.em-grade.com\/es\/producto\/stain77-emstain-solution\/","title":{"rendered":"STAIN77 – EMstain solution"},"content":{"rendered":"

Product description :<\/strong><\/h4>\n

Stain77 is an extremely low-toxicity contrast agent, consisting of lithium and tungsten salts (refer to MSDS). It effectively enhances both positive and negative contrast. It remains insensitive to light and allows for long-term storage at room temperature (RT). If crystallization occurs over time, you can restore the product’s contrast-enhancing properties through filtration or centrifugation.<\/p>\n

Application :<\/strong><\/h4>\n

Stain77 serves as a genuine alternative to uranyl acetate for section contrast and negative staining, boasting a pH near 7 and ensuring user safety. Contrast results, after enhancing with lead citrate, match or even surpass those of uranyl acetate.<\/p>\n

The Stain77 Ultra-Thin Section Contrast Protocol involves:<\/p>\n

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  1. Applying a drop of Stain77 for 1 to 2 minutes.<\/li>\n
  2. Drying.<\/li>\n
  3. Briefly rinsing with distilled water.<\/li>\n
  4. Drying.<\/li>\n
  5. Counterstaining with lead citrate for 1 minute, followed by rinsing with distilled water and drying.<\/li>\n
  6. Observation using a Transmission Electron Microscope (TEM).<\/li>\n<\/ol>\n

    Please note: Avoid pre-storing rinse water in the refrigerator, as cold water may absorb CO2, potentially contaminating your sections.<\/p>\n

    The protocol above enhances the contrast of ultra-thin sections (positive contrast) and is also suitable for negative staining. However, negative staining does not require counterstaining with lead citrate after using Stain77.<\/p>\n

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    Tips :<\/strong><\/h4>\n

    It’s available in two variants and two volumes (30ml and 200ml):<\/p>\n