{"id":3558,"date":"2023-09-06T12:08:41","date_gmt":"2023-09-06T10:08:41","guid":{"rendered":"https:\/\/www.em-grade.com\/questions-answers\/"},"modified":"2024-02-27T09:37:03","modified_gmt":"2024-02-27T08:37:03","slug":"faq","status":"publish","type":"page","link":"https:\/\/www.em-grade.com\/fr\/faq\/","title":{"rendered":"FAQ"},"content":{"rendered":"[vc_row type=\u00a0\u00bbfull_width_content\u00a0\u00bb full_screen_row_position=\u00a0\u00bbmiddle\u00a0\u00bb column_margin=\u00a0\u00bb50px\u00a0\u00bb equal_height=\u00a0\u00bbyes\u00a0\u00bb content_placement=\u00a0\u00bbmiddle\u00a0\u00bb column_direction=\u00a0\u00bbdefault\u00a0\u00bb column_direction_tablet=\u00a0\u00bbdefault\u00a0\u00bb column_direction_phone=\u00a0\u00bbdefault\u00a0\u00bb bg_image=\u00a0\u00bb1113″ bg_image_phone=\u00a0\u00bb1295″ bg_position=\u00a0\u00bbleft center\u00a0\u00bb background_image_loading=\u00a0\u00bbdefault\u00a0\u00bb bg_repeat=\u00a0\u00bbno-repeat\u00a0\u00bb 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width=\u00a0\u00bb2\/3″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb enable_animation=\u00a0\u00bbtrue\u00a0\u00bb animation=\u00a0\u00bbfade-in\u00a0\u00bb animation_easing=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb column_padding_type=\u00a0\u00bbdefault\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb][vc_row_inner column_margin=\u00a0\u00bbdefault\u00a0\u00bb column_direction=\u00a0\u00bbdefault\u00a0\u00bb column_direction_tablet=\u00a0\u00bbdefault\u00a0\u00bb column_direction_phone=\u00a0\u00bbdefault\u00a0\u00bb text_align=\u00a0\u00bbleft\u00a0\u00bb row_position=\u00a0\u00bbdefault\u00a0\u00bb row_position_tablet=\u00a0\u00bbinherit\u00a0\u00bb row_position_phone=\u00a0\u00bbinherit\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb 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tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb column_padding_type=\u00a0\u00bbdefault\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb][split_line_heading animation_type=\u00a0\u00bbline-reveal-by-space\u00a0\u00bb font_style=\u00a0\u00bbh1″ text_color=\u00a0\u00bb#2c3338″ line_reveal_by_space_text_effect=\u00a0\u00bbdefault\u00a0\u00bb content_alignment=\u00a0\u00bbdefault\u00a0\u00bb mobile_content_alignment=\u00a0\u00bbinherit\u00a0\u00bb link_target=\u00a0\u00bb_self\u00a0\u00bb text_content=\u00a0\u00bbFrequently asked questions\u00a0\u00bb font_size=\u00a0\u00bb60px\u00a0\u00bb font_line_height=\u00a0\u00bb110%\u00a0\u00bb font_size_tablet=\u00a0\u00bb45″][\/split_line_heading][vc_column_text el_class=\u00a0\u00bbmed\u00a0\u00bb text_direction=\u00a0\u00bbdefault\u00a0\u00bb]En savoir plus[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][vc_column column_padding=\u00a0\u00bbno-extra-padding\u00a0\u00bb column_padding_tablet=\u00a0\u00bbinherit\u00a0\u00bb column_padding_phone=\u00a0\u00bbinherit\u00a0\u00bb column_padding_position=\u00a0\u00bball\u00a0\u00bb column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bbdefault\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_hover_color_opacity=\u00a0\u00bb1″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb font_color=\u00a0\u00bb#ffffff\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bbnone\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb 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top_padding_phone=\u00a0\u00bb15%\u00a0\u00bb text_color=\u00a0\u00bblight\u00a0\u00bb text_align=\u00a0\u00bbleft\u00a0\u00bb row_border_radius=\u00a0\u00bbnone\u00a0\u00bb row_border_radius_applies=\u00a0\u00bbbg\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb shape_divider_position=\u00a0\u00bbbottom\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb shape_type=\u00a0\u00bb\u00a0\u00bb][vc_column column_padding=\u00a0\u00bbno-extra-padding\u00a0\u00bb column_padding_tablet=\u00a0\u00bbinherit\u00a0\u00bb column_padding_phone=\u00a0\u00bbinherit\u00a0\u00bb column_padding_position=\u00a0\u00bball\u00a0\u00bb column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bbdefault\u00a0\u00bb centered_text=\u00a0\u00bbtrue\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_hover_color_opacity=\u00a0\u00bb1″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bbnone\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb column_position=\u00a0\u00bbdefault\u00a0\u00bb gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ width=\u00a0\u00bb1\/1″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb column_padding_type=\u00a0\u00bbdefault\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb][vc_row_inner equal_height=\u00a0\u00bbyes\u00a0\u00bb content_placement=\u00a0\u00bbmiddle\u00a0\u00bb column_margin=\u00a0\u00bb10px\u00a0\u00bb column_direction=\u00a0\u00bbdefault\u00a0\u00bb column_direction_tablet=\u00a0\u00bbdefault\u00a0\u00bb column_direction_phone=\u00a0\u00bbdefault\u00a0\u00bb text_align=\u00a0\u00bbleft\u00a0\u00bb row_position=\u00a0\u00bbdefault\u00a0\u00bb row_position_tablet=\u00a0\u00bbinherit\u00a0\u00bb row_position_phone=\u00a0\u00bbinherit\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb pointer_events=\u00a0\u00bball\u00a0\u00bb][vc_column_inner top_padding_desktop=\u00a0\u00bb15″ constrain_group_100=\u00a0\u00bbyes\u00a0\u00bb bottom_padding_desktop=\u00a0\u00bb15″ left_padding_desktop=\u00a0\u00bb15″ constrain_group_101=\u00a0\u00bbyes\u00a0\u00bb right_padding_desktop=\u00a0\u00bb15″ column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bbdefault\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color=\u00a0\u00bb#ee443c\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_color_hover=\u00a0\u00bb#ee443c\u00a0\u00bb background_hover_color_opacity=\u00a0\u00bb0.7″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bb100px\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ width=\u00a0\u00bb1\/5″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb column_padding_type=\u00a0\u00bbadvanced\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb column_link=\u00a0\u00bb#chemistry\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bb\u00a0\u00bb]Chimie, D\u00e9finitions de base …[\/vc_column_text][\/vc_column_inner][vc_column_inner top_padding_desktop=\u00a0\u00bb15″ constrain_group_100=\u00a0\u00bbyes\u00a0\u00bb bottom_padding_desktop=\u00a0\u00bb15″ left_padding_desktop=\u00a0\u00bb15″ constrain_group_101=\u00a0\u00bbyes\u00a0\u00bb right_padding_desktop=\u00a0\u00bb15″ top_margin_phone=\u00a0\u00bb-10″ column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bbdefault\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color=\u00a0\u00bb#ee443c\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_color_hover=\u00a0\u00bb#ee443c\u00a0\u00bb background_hover_color_opacity=\u00a0\u00bb0.7″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bb100px\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ width=\u00a0\u00bb1\/5″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb column_padding_type=\u00a0\u00bbadvanced\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb column_link=\u00a0\u00bb#laboratory\u00a0\u00bb][vc_column_text el_class=\u00a0\u00bbuppercase\u00a0\u00bb text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Eau de laboratoire[\/vc_column_text][\/vc_column_inner][vc_column_inner top_padding_desktop=\u00a0\u00bb15″ constrain_group_100=\u00a0\u00bbyes\u00a0\u00bb bottom_padding_desktop=\u00a0\u00bb15″ left_padding_desktop=\u00a0\u00bb15″ constrain_group_101=\u00a0\u00bbyes\u00a0\u00bb right_padding_desktop=\u00a0\u00bb15″ top_margin_phone=\u00a0\u00bb-10″ column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bbdefault\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color=\u00a0\u00bb#ee443c\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_color_hover=\u00a0\u00bb#ee443c\u00a0\u00bb background_hover_color_opacity=\u00a0\u00bb0.7″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bb100px\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ width=\u00a0\u00bb1\/5″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb column_padding_type=\u00a0\u00bbadvanced\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb column_link=\u00a0\u00bb#buffer\u00a0\u00bb][vc_column_text el_class=\u00a0\u00bbuppercase\u00a0\u00bb text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Tampon[\/vc_column_text][\/vc_column_inner][vc_column_inner top_padding_desktop=\u00a0\u00bb15″ constrain_group_100=\u00a0\u00bbyes\u00a0\u00bb bottom_padding_desktop=\u00a0\u00bb15″ left_padding_desktop=\u00a0\u00bb15″ constrain_group_101=\u00a0\u00bbyes\u00a0\u00bb right_padding_desktop=\u00a0\u00bb15″ top_margin_phone=\u00a0\u00bb-10″ column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bbdefault\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color=\u00a0\u00bb#ee443c\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_color_hover=\u00a0\u00bb#ee443c\u00a0\u00bb background_hover_color_opacity=\u00a0\u00bb0.7″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bb100px\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ width=\u00a0\u00bb1\/5″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb 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Solutions fixatives<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row type=\u00a0\u00bbin_container\u00a0\u00bb full_screen_row_position=\u00a0\u00bbmiddle\u00a0\u00bb column_margin=\u00a0\u00bbdefault\u00a0\u00bb column_direction=\u00a0\u00bbdefault\u00a0\u00bb column_direction_tablet=\u00a0\u00bbdefault\u00a0\u00bb column_direction_phone=\u00a0\u00bbdefault\u00a0\u00bb scene_position=\u00a0\u00bbcenter\u00a0\u00bb top_padding=\u00a0\u00bb50″ text_color=\u00a0\u00bbdark\u00a0\u00bb text_align=\u00a0\u00bbleft\u00a0\u00bb row_border_radius=\u00a0\u00bbnone\u00a0\u00bb row_border_radius_applies=\u00a0\u00bbbg\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb id=\u00a0\u00bbchemistry\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb shape_divider_position=\u00a0\u00bbbottom\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb shape_type=\u00a0\u00bb\u00a0\u00bb][vc_column column_padding=\u00a0\u00bbno-extra-padding\u00a0\u00bb column_padding_tablet=\u00a0\u00bbinherit\u00a0\u00bb column_padding_phone=\u00a0\u00bbinherit\u00a0\u00bb column_padding_position=\u00a0\u00bball\u00a0\u00bb column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bb5px\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_hover_color_opacity=\u00a0\u00bb1″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bbnone\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb column_position=\u00a0\u00bbdefault\u00a0\u00bb gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ width=\u00a0\u00bb1\/1″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb column_padding_type=\u00a0\u00bbdefault\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb][vc_column_text css_animation=\u00a0\u00bbfadeInUp\u00a0\u00bb el_class=\u00a0\u00bbtitle\u00a0\u00bb text_direction=\u00a0\u00bbdefault\u00a0\u00bb]\n

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Chimie, D\u00e9finitions de base, pH, Molarit\u00e9…<\/h2>\n<\/div>\n<\/div>\n<\/div>\n
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 <\/p>\n

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<\/div>\n<\/div>\n<\/form>\n<\/div>\n[\/vc_column_text][toggles style=\u00a0\u00bbminimal\u00a0\u00bb accordion=\u00a0\u00bbtrue\u00a0\u00bb accordion_starting_functionality=\u00a0\u00bbclosed\u00a0\u00bb][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbTemp\u00e9rature, temps de r\u00e9action\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Pour une bonne pratique exp\u00e9rimentale en laboratoire, il est imp\u00e9ratif de prendre en compte la temp\u00e9rature de vos r\u00e9actifs. Permettez toujours \u00e0 vos r\u00e9actifs de revenir \u00e0 temp\u00e9rature ambiante dans le laboratoire (g\u00e9n\u00e9ralement entre 18 et 22 \u00b0C) avant de commencer votre protocole exp\u00e9rimental. Cela garantira une meilleure reproductibilit\u00e9 de vos r\u00e9sultats, en tenant compte non seulement des concentrations, mais aussi des param\u00e8tres de temp\u00e9rature et de temps de r\u00e9action.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbEau de laboratoire\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]L’eau est essentielle \u00e0 la recherche en sciences de la vie, mais pour de nombreux scientifiques, la r\u00e9flexion la plus importante sur ce r\u00e9actif de laboratoire le plus largement utilis\u00e9 est \u00ab\u00a0combien en ai-je besoin ?\u00a0\u00bb Parce que l’eau est capable de servir de solvant pour de nombreuses substances et r\u00e9actifs (solution aqueuse), m\u00e9fiez-vous, elle peut devenir contamin\u00e9e. L’eau peut \u00e9galement \u00eatre un milieu de dilution.<\/p>\n

M\u00eame au sein du m\u00eame laboratoire, l’eau utilis\u00e9e dans une exp\u00e9rience peut diff\u00e9rer de celle utilis\u00e9e dans une autre. Il existe une multitude de m\u00e9thodes, d’appareils et de moyens de produire de l’eau purifi\u00e9e, nous devrons donc sp\u00e9cifier les moyens de production de cette eau. Toutes les eaux ne sont pas identiques, donc consid\u00e9rez l’eau comme un r\u00e9actif de laboratoire et examinez-la en cons\u00e9quence. Comprendre l’importance de la qualit\u00e9 de l’eau et la surveiller de pr\u00e8s est important pour garantir la reproductibilit\u00e9 exp\u00e9rimentale sur plusieurs jours, mois et m\u00eame ann\u00e9es.<\/p>\n

, months and even years.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbNum\u00e9ro CAS\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]\n

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Le num\u00e9ro CAS (ou num\u00e9ro d’enregistrement CAS) d’un produit chimique, d’un polym\u00e8re, d’une s\u00e9quence biologique ou d’un alliage est son num\u00e9ro d’enregistrement unique dans la base de donn\u00e9es du Chemical Abstracts Service (CAS), une division de l’American Chemical Society (ACS). Le CAS attribue ces num\u00e9ros \u00e0 chaque substance chimique d\u00e9crite dans la documentation. Le CAS g\u00e8re \u00e9galement et commercialise une base de donn\u00e9es de ces substances, le Registre CAS.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbProduit chimique ACS\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]La Soci\u00e9t\u00e9 am\u00e9ricaine de chimie (ACS), avec plus de 150 000 membres, est l’une des plus grandes organisations scientifiques du monde et l’une des principales sources d’informations scientifiques fiables sur la chimie.<\/p>\n

Elle publie des r\u00e9f\u00e9rences, commande et d\u00e9crit chaque produit ACS selon une m\u00e9thode normalis\u00e9e. Si vous avez besoin d’informations plus d\u00e9taill\u00e9es sur un produit chimique portant l’\u00e9tiquette ACS, vous pouvez trouver sa composition exacte dans la base de donn\u00e9es disponible gratuitement sur le site web https:\/\/www.acs.org\/content\/acs\/en.html<\/a>.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbMicrofiltr\u00e9?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Les solutions microfiltr\u00e9es sont produites par filtration sous vide sur une membrane (dispositif de filtration). Il existe de nombreux types de membranes (diam\u00e8tre, porosit\u00e9, mat\u00e9riaux, etc.).<\/p>\n

Les membranes utilis\u00e9es pour filtrer une solution aqueuse sont fabriqu\u00e9es \u00e0 partir d’un filtre \u00e0 base de cellulose (ester de cellulose), avec un diam\u00e8tre adapt\u00e9 \u00e0 votre dispositif de filtration et une porosit\u00e9 de 0,4 \u00b5 ou 0,2 \u00b5 (la porosit\u00e9 de 0,2 \u00b5 permet \u00e9galement de retenir les bact\u00e9ries (d’o\u00f9 la m\u00e9thode de st\u00e9rilisation).[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbChoisir votre pH\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Les compartiments cellulaires fonctionnent \u00e0 des niveaux de pH proches de 7. Il n’y a pas de pH universel pour la fixation.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbQuelle est la diff\u00e9rence entre une solution m\u00e8re et une solution fille ?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Une solution m\u00e8re est g\u00e9n\u00e9ralement une solution concentr\u00e9e qui peut \u00eatre dilu\u00e9e pour obtenir des solutions filles moins concentr\u00e9es. Par exemple : pr\u00e9parer une solution m\u00e8re de 0,2 M pour obtenir un tampon de phosphate de 0,1 M (solution fille), une solution m\u00e8re de 0,4 M pour obtenir une solution fille de 0,2 M.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbQuelle est la date de p\u00e9remption d’un produit ?\u00a0\u00bb][vc_column_text]All products on the market generally have an expiration date. This doesn’t necessarily mean you can’t use them after that date. The expiration date is the date on which the supplier’s warranty on your product expires.<\/p>\n

We’ve all tested this, sometimes using chemicals that have been out of date for several years, and the result was excellent (it generated less waste). It’s important to remember that product shelf-life is highly dependent on packaging and storage.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbQuel volume et quel conditionnement ?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]EM-grade vise \u00e0 \u00eatre un acteur \u00e9co-responsable dans le secteur de la microscopie \u00e9lectronique, en promouvant l’utilisation rationnelle de produits chimiques ayant un double impact sur notre environnement et notre sant\u00e9.<\/p>\n

Si nous ne pouvons pas nous passer de certains produits parfois toxiques, alors consommons-les mieux et moins souvent.<\/p>\n

C’est pourquoi nous pr\u00e9f\u00e9rons vendre des produits en petits volumes et dans des emballages de petite taille, afin d’\u00e9viter de les stocker pendant des ann\u00e9es dans des d\u00e9chets de laboratoire.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbPourquoi choisir des emballages de petite taille ?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Les professionnels de laboratoire ne sont pas de grands consommateurs de petits conditionnements pour deux raisons principales :<\/p>\n

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  1. La crainte de manquer de produit pendant les op\u00e9rations de manipulation.<\/li>\n
  2. Le co\u00fbt plus \u00e9lev\u00e9 des produits par rapport aux achats en gros.<\/li>\n<\/ol>\n

    EM grade garantit l’exp\u00e9dition dans les 24 heures (pas de pannes) et propose des prix raisonnables pour toutes les quantit\u00e9s.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbTable de Mendele\u00efev\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]La Table p\u00e9riodique des \u00e9l\u00e9ments, \u00e9galement connue sous le nom de Table de Mendele\u00efev, la Table p\u00e9riodique ou simplement la Table p\u00e9riodique, repr\u00e9sente tous les \u00e9l\u00e9ments chimiques, ordonn\u00e9s par num\u00e9ro atomique croissant et organis\u00e9s selon leur configuration \u00e9lectronique, qui sous-tend leurs propri\u00e9t\u00e9s chimiques.<\/p>\n

    La conception de cette table est g\u00e9n\u00e9ralement attribu\u00e9e au chimiste russe Dmitri Ivanovitch Mendele\u00efev.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbNeutral gases\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Un gaz neutre (ou inert) est un gaz stable (ce qui signifie qu’il poss\u00e8de d\u00e9j\u00e0 8 \u00e9lectrons de valence dans sa couche externe et n’a pas besoin de r\u00e9agir pour atteindre ces 8 \u00e9lectrons). Ces gaz sont \u00e9galement connus sous le nom de gaz nobles ou gaz rares. Ils n’initient pas de r\u00e9actions chimiques par eux-m\u00eames (l’azote est un gaz inerte, tout comme l’argon, le plus largement utilis\u00e9 dans les conditions EM-grade).[\/vc_column_text][\/toggle][\/toggles][\/vc_column][\/vc_row][vc_row type=\u00a0\u00bbin_container\u00a0\u00bb full_screen_row_position=\u00a0\u00bbmiddle\u00a0\u00bb column_margin=\u00a0\u00bbdefault\u00a0\u00bb column_direction=\u00a0\u00bbdefault\u00a0\u00bb column_direction_tablet=\u00a0\u00bbdefault\u00a0\u00bb column_direction_phone=\u00a0\u00bbdefault\u00a0\u00bb scene_position=\u00a0\u00bbcenter\u00a0\u00bb top_padding=\u00a0\u00bb50″ text_color=\u00a0\u00bbdark\u00a0\u00bb text_align=\u00a0\u00bbleft\u00a0\u00bb row_border_radius=\u00a0\u00bbnone\u00a0\u00bb row_border_radius_applies=\u00a0\u00bbbg\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb id=\u00a0\u00bblaboratory\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb shape_divider_position=\u00a0\u00bbbottom\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb shape_type=\u00a0\u00bb\u00a0\u00bb][vc_column column_padding=\u00a0\u00bbno-extra-padding\u00a0\u00bb column_padding_tablet=\u00a0\u00bbinherit\u00a0\u00bb column_padding_phone=\u00a0\u00bbinherit\u00a0\u00bb column_padding_position=\u00a0\u00bball\u00a0\u00bb column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bb5px\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_hover_color_opacity=\u00a0\u00bb1″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bbnone\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb column_position=\u00a0\u00bbdefault\u00a0\u00bb gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ width=\u00a0\u00bb1\/1″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb column_padding_type=\u00a0\u00bbdefault\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb][vc_column_text css_animation=\u00a0\u00bbfadeInUp\u00a0\u00bb el_class=\u00a0\u00bbtitle\u00a0\u00bb text_direction=\u00a0\u00bbdefault\u00a0\u00bb]\n

    L’eau de laboratoire<\/h2>\n[\/vc_column_text][toggles style=\u00a0\u00bbminimal\u00a0\u00bb accordion=\u00a0\u00bbtrue\u00a0\u00bb accordion_starting_functionality=\u00a0\u00bbclosed\u00a0\u00bb][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbPourquoi la solution tampon cristallise-t-elle ?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Lorsque la solution tampon a une concentration \u00e9lev\u00e9e, \u00e0 partir de 0,4 molaire et au-dessus, elle peut cristalliser dans une certaine mesure lorsqu’elle est stock\u00e9e au r\u00e9frig\u00e9rateur. Cette condition est r\u00e9versible, car les cristaux se dissoudront en quelques heures \u00e0 temp\u00e9rature ambiante. Vous pouvez la r\u00e9chauffer pour acc\u00e9l\u00e9rer la disparition des cristaux. Cette cristallisation ne change pas les propri\u00e9t\u00e9s chimiques de la solution tampon.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbPBS (Phosphate Buffered Saline) ou DPBS (Dulbecco’s Phosphate Buffered Saline), quelle est la diff\u00e9rence ?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]DPBS (Dulbecco’s Phosphate Buffered Saline)<\/p>\n

    PBS (Phosphate Buffered Saline). En raison des contraintes d’osmolarit\u00e9 associ\u00e9es au fixateur, ou \u00e0 des fins de rin\u00e7age, il est g\u00e9n\u00e9ralement pr\u00e9par\u00e9 \u00e0 0,1 M ou 0,2 M \u00e0 un pH d’environ 7 \u00e0 7,4, pour compenser l’acidit\u00e9 l\u00e9g\u00e8re lib\u00e9r\u00e9e par les cellules pendant la fixation.<\/p>\n

    DPBS (d\u00e9velopp\u00e9 par Dulbecco) peut contenir des additifs de CaCl2 et de MgCl2 pour faciliter le d\u00e9tachement des cellules en culture. Si le DPBS ne contient pas ces additifs, il peut \u00eatre remplac\u00e9 par du PBS.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbQuels sont les effets de CaCl2 et MgCl2 sur la physiologie cellulaire ?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]\n

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    Le calcium et le magn\u00e9sium facilitent l’adh\u00e9sion cellulaire et l’agglutination (facilitant la r\u00e9cup\u00e9ration cellulaire en culture sur plaque).<\/p>\n

    Veuillez noter :<\/p>\n

    D-PBS a g\u00e9n\u00e9ralement une concentration l\u00e9g\u00e8rement plus faible en phosphate que PBS, DPBS peut inclure ou non du calcium et\/ou du magn\u00e9sium. Les besoins exp\u00e9rimentaux dicteront quelle solution utiliser.<\/p>\n

    Par exemple, si dans une exp\u00e9rience particuli\u00e8re l’activit\u00e9 enzymatique de la trypsine doit \u00eatre mesur\u00e9e, le calcium et le magn\u00e9sium parfois inclus dans le DPBS peuvent fausser les r\u00e9sultats, il serait donc pr\u00e9f\u00e9rable d’utiliser du PBS simple.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbPourquoi utiliser un tampon \u00e0 base de phosphate ?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]\n

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    Les tampons phosphate sont les solutions les plus largement utilis\u00e9es en biologie, car elles sont non toxiques.<\/p>\n

    Cependant, cette non-toxicit\u00e9 peut encourager le d\u00e9veloppement de micro-organismes \u00e0 moyen terme dans la solution. Cet inconv\u00e9nient peut \u00eatre surmont\u00e9 en pr\u00e9parant des solutions m\u00e8res avec des concentrations \u00e9lev\u00e9es de 0,4 M, 1 M et plus, car les micro-organismes ne prolif\u00e8rent pas dans des concentrations \u00e9lev\u00e9es en sels (avec quelques exceptions).<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbMon tampon phosphate semble contamin\u00e9. Que puis-je faire ?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Les tampons \u00e0 base de phosphate deviennent contamin\u00e9s apr\u00e8s quelques mois (2 \u00e0 3 mois maximum), car ils sont vitaux. Ils peuvent \u00eatre d\u00e9contamin\u00e9s et continuer \u00e0 \u00eatre utilis\u00e9s simplement en filtrant \u00e0 0,22 \u00b5 (filtre seringue, etc.). Stockez \u00e0 4 – 8\u00b0C.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbPourquoi utiliser du cacodylate de sodium ?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Ce n’est pas un tampon vital, car il contient des traces d’\u00e9l\u00e9ments toxiques tels que l’arsenic.<\/p>\n

    Cependant, il est encore largement utilis\u00e9 lors de la fixation des cellules (puisque la fixation tue la cellule). Dans ce cas, la toxicit\u00e9 du cacodylate n’est plus un inconv\u00e9nient. Au contraire, sa toxicit\u00e9 devient un avantage, gr\u00e2ce \u00e0 sa facilit\u00e9 de pr\u00e9paration. Il a une longue dur\u00e9e de conservation (toxique pour les micro-organismes) et ne n\u00e9cessite pas de st\u00e9rilisation.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbTampon \u00e0 l’imidazole, quel est son int\u00e9r\u00eat ? Les lipides !\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]L’utilit\u00e9 de l’osmium t\u00e9troxyde tamponn\u00e9 \u00e0 l’imidazole comme conservateur des lipides en microscopie \u00e9lectronique en transmission est vivement recommand\u00e9e, car l’imidazole permet la r\u00e9tention et le maintien des lipides m\u00eame apr\u00e8s d\u00e9shydratation alcoolique. Par exemple, le foie de rat et d’autres tissus ont \u00e9t\u00e9 fix\u00e9s par perfusion avec du glutarald\u00e9hyde et post-fix\u00e9s avec de l’osmium-imidazole, et l’apparence des gouttelettes lipidiques a \u00e9t\u00e9 compar\u00e9e \u00e0 celle apr\u00e8s fixation dans du t\u00e9troxyde d’osmium aqueux non tamponn\u00e9 avec de l’imidazole. Une coloration \u00e9lectroniquement opaque des gouttelettes lipidiques et des particules de lipoprot\u00e9ines a \u00e9t\u00e9 remarqu\u00e9e apr\u00e8s post-fixation avec 1% d’osmium-imidazole \u00e0 0,1 M, pH 7,5, pendant 30 minutes. (plus de d\u00e9tails voir protocole).[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbComment mieux g\u00e9rer vos stocks et achats ?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]G\u00e9rez mieux vos inventaires chimiques avec un inventaire quantifi\u00e9 simple pour anticiper vos achats.<\/p>\n

    Regroupez vos achats pour \u00e9conomiser de l’argent, mais surtout pour limiter l’impact carbone sur la plan\u00e8te.<\/p>\n

    Nous n\u00e9gocions souvent les co\u00fbts de livraison sans tenir compte de l’impact sur notre environnement.<\/p>\n

    EM-grade vous accordera une r\u00e9duction sur les frais de livraison pour toutes les commandes group\u00e9es.[\/vc_column_text][\/toggle][\/toggles][\/vc_column][\/vc_row][vc_row type=\u00a0\u00bbin_container\u00a0\u00bb full_screen_row_position=\u00a0\u00bbmiddle\u00a0\u00bb column_margin=\u00a0\u00bbdefault\u00a0\u00bb column_direction=\u00a0\u00bbdefault\u00a0\u00bb column_direction_tablet=\u00a0\u00bbdefault\u00a0\u00bb column_direction_phone=\u00a0\u00bbdefault\u00a0\u00bb scene_position=\u00a0\u00bbcenter\u00a0\u00bb top_padding=\u00a0\u00bb50″ text_color=\u00a0\u00bbdark\u00a0\u00bb text_align=\u00a0\u00bbleft\u00a0\u00bb row_border_radius=\u00a0\u00bbnone\u00a0\u00bb row_border_radius_applies=\u00a0\u00bbbg\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb id=\u00a0\u00bbbuffer\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb shape_divider_position=\u00a0\u00bbbottom\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb shape_type=\u00a0\u00bb\u00a0\u00bb][vc_column column_padding=\u00a0\u00bbno-extra-padding\u00a0\u00bb column_padding_tablet=\u00a0\u00bbinherit\u00a0\u00bb column_padding_phone=\u00a0\u00bbinherit\u00a0\u00bb column_padding_position=\u00a0\u00bball\u00a0\u00bb column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bb5px\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_hover_color_opacity=\u00a0\u00bb1″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bbnone\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb column_position=\u00a0\u00bbdefault\u00a0\u00bb gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ width=\u00a0\u00bb1\/1″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb column_padding_type=\u00a0\u00bbdefault\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb][vc_column_text css_animation=\u00a0\u00bbfadeInUp\u00a0\u00bb el_class=\u00a0\u00bbtitle\u00a0\u00bb text_direction=\u00a0\u00bbdefault\u00a0\u00bb]\n

    Tampon<\/h2>\n[\/vc_column_text][toggles style=\u00a0\u00bbminimal\u00a0\u00bb accordion=\u00a0\u00bbtrue\u00a0\u00bb accordion_starting_functionality=\u00a0\u00bbclosed\u00a0\u00bb][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbLes tampons Normand GOOD\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]En 1966, Norman Good et ses coll\u00e8gues ont entrepris de d\u00e9finir les meilleurs tampons pour les syst\u00e8mes biochimiques.<\/p>\n

    En 1980, Good et ses coll\u00e8gues ont identifi\u00e9 vingt tampons qui ont \u00e9tabli la norme pour une utilisation dans la recherche biologique et biochimique que nous utilisons maintenant en microscopie pour la fixation des tissus.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbMon tampon semble contamin\u00e9 ! Que puis-je faire \u00e0 ce sujet ?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Parfois, le stockage de vos tampons vitaux (phosphate, PHEM, etc.) dont la date de p\u00e9remption (garantie) est d\u00e9pass\u00e9e peut \u00eatre contamin\u00e9 par la prolif\u00e9ration de bact\u00e9ries ou de moisissures. Cet \u00e9tat de contamination ne diminue en rien les propri\u00e9t\u00e9s de vos tampons. Il suffit de les refiltrer \u00e0 0,22 \u00b5m ou d’autoclaver votre solution tampon.<\/p>\n

    Une fois trait\u00e9s, vos tampons sont s\u00fbrs \u00e0 utiliser.[\/vc_column_text][\/toggle][\/toggles][\/vc_column][\/vc_row][vc_row type=\u00a0\u00bbin_container\u00a0\u00bb full_screen_row_position=\u00a0\u00bbmiddle\u00a0\u00bb column_margin=\u00a0\u00bbdefault\u00a0\u00bb column_direction=\u00a0\u00bbdefault\u00a0\u00bb column_direction_tablet=\u00a0\u00bbdefault\u00a0\u00bb column_direction_phone=\u00a0\u00bbdefault\u00a0\u00bb scene_position=\u00a0\u00bbcenter\u00a0\u00bb top_padding=\u00a0\u00bb50″ text_color=\u00a0\u00bbdark\u00a0\u00bb text_align=\u00a0\u00bbleft\u00a0\u00bb row_border_radius=\u00a0\u00bbnone\u00a0\u00bb row_border_radius_applies=\u00a0\u00bbbg\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb id=\u00a0\u00bbemgrade\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb shape_divider_position=\u00a0\u00bbbottom\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb shape_type=\u00a0\u00bb\u00a0\u00bb][vc_column column_padding=\u00a0\u00bbno-extra-padding\u00a0\u00bb column_padding_tablet=\u00a0\u00bbinherit\u00a0\u00bb column_padding_phone=\u00a0\u00bbinherit\u00a0\u00bb column_padding_position=\u00a0\u00bball\u00a0\u00bb column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bb5px\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_hover_color_opacity=\u00a0\u00bb1″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bbnone\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb column_position=\u00a0\u00bbdefault\u00a0\u00bb gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ width=\u00a0\u00bb1\/1″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb column_padding_type=\u00a0\u00bbdefault\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb][vc_column_text css_animation=\u00a0\u00bbfadeInUp\u00a0\u00bb el_class=\u00a0\u00bbtitle\u00a0\u00bb]\n

    EM grade<\/h2>\n[\/vc_column_text][toggles style=\u00a0\u00bbminimal\u00a0\u00bb accordion=\u00a0\u00bbtrue\u00a0\u00bb accordion_starting_functionality=\u00a0\u00bbclosed\u00a0\u00bb][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbQu’est ce que EM grade?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]EM-grade signifie que ce produit est qualifi\u00e9 pour \u00eatre utilis\u00e9 dans la pr\u00e9paration d’\u00e9chantillons \u00e0 examiner sous un microscope \u00e9lectronique.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbQuelle est la norme EM-grade (conditions) ?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Un produit de qualit\u00e9 EM doit \u00eatre :<\/p>\n
      \n
    • d’une grande puret\u00e9 chimique et distill\u00e9,<\/li>\n
    • scell\u00e9 dans une atmosph\u00e8re neutre (satur\u00e9e d’un gaz neutre tel que l’argon ou l’azote) sans contact avec l’air, que ce soit l’oxyg\u00e8ne ou le CO2 (sans air).<\/li>\n<\/ul>\n[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbComment scellez-vous dans une atmosph\u00e8re neutre ?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Pour de petits volumes, scellez dans une ampoule ou une bouteille sans air.<\/p>\n

      Pour des volumes plus importants, nous injectons de l’argon ou de l’azote, saturant le liquide en le faisant barboter dans la solution embouteill\u00e9e pour remplacer l’oxyg\u00e8ne ou le dioxyde de carbone contenu dans l’eau.<\/p>\n

      Mais lorsque vous ouvrez la bouteille, vous perdez l’atmosph\u00e8re neutre. C’est pourquoi nous vendons de petits volumes pour \u00e9viter le gaspillage.<\/p>\n

      Il n’y a aucun risque de manquer de vos produits pour vos exp\u00e9riences, car ils sont produits le jour de votre commande et exp\u00e9di\u00e9s imm\u00e9diatement.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbPourquoi choisir des gaz neutres ?\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]\n

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      L’air contient de l’oxyg\u00e8ne, le principal agent oxydant dans la nature, qui d\u00e9grade les composants de votre produit.<\/p>\n

      L’oxyg\u00e8ne et un peu de CO2 (Air) sont remplac\u00e9s par des gaz neutres tels que l’argon ou l’azote.<\/p>\n

      Cela permet \u00e0 votre produit de se conserver plus longtemps, si vous ne ouvrez pas la bouteille. Pour cette raison, il est pr\u00e9f\u00e9rable d’acheter de petits volumes, afin d’avoir toujours des produits efficaces lorsque vous en avez besoin.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[\/vc_column_text][\/toggle][\/toggles][\/vc_column][\/vc_row][vc_row type=\u00a0\u00bbin_container\u00a0\u00bb full_screen_row_position=\u00a0\u00bbmiddle\u00a0\u00bb column_margin=\u00a0\u00bbdefault\u00a0\u00bb column_direction=\u00a0\u00bbdefault\u00a0\u00bb column_direction_tablet=\u00a0\u00bbdefault\u00a0\u00bb column_direction_phone=\u00a0\u00bbdefault\u00a0\u00bb scene_position=\u00a0\u00bbcenter\u00a0\u00bb top_padding=\u00a0\u00bb50″ bottom_padding=\u00a0\u00bb5%\u00a0\u00bb text_color=\u00a0\u00bbdark\u00a0\u00bb text_align=\u00a0\u00bbleft\u00a0\u00bb row_border_radius=\u00a0\u00bbnone\u00a0\u00bb row_border_radius_applies=\u00a0\u00bbbg\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb id=\u00a0\u00bbfixative\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb shape_divider_position=\u00a0\u00bbbottom\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb shape_type=\u00a0\u00bb\u00a0\u00bb][vc_column column_padding=\u00a0\u00bbno-extra-padding\u00a0\u00bb column_padding_tablet=\u00a0\u00bbinherit\u00a0\u00bb column_padding_phone=\u00a0\u00bbinherit\u00a0\u00bb column_padding_position=\u00a0\u00bball\u00a0\u00bb column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bb5px\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_hover_color_opacity=\u00a0\u00bb1″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bbnone\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb column_position=\u00a0\u00bbdefault\u00a0\u00bb gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ width=\u00a0\u00bb1\/1″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb column_padding_type=\u00a0\u00bbdefault\u00a0\u00bb gradient_type=\u00a0\u00bbdefault\u00a0\u00bb][vc_column_text css_animation=\u00a0\u00bbfadeInUp\u00a0\u00bb el_class=\u00a0\u00bbtitle\u00a0\u00bb text_direction=\u00a0\u00bbdefault\u00a0\u00bb]\n

      Solutions de fixation<\/h2>\n[\/vc_column_text][toggles style=\u00a0\u00bbminimal\u00a0\u00bb accordion=\u00a0\u00bbtrue\u00a0\u00bb accordion_starting_functionality=\u00a0\u00bbclosed\u00a0\u00bb][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbD\u00e9finition de la fixation\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Fixer les cellules signifie les conserver dans un \u00e9tat aussi proche que possible de leur \u00e9tat vivant. Des produits chimiques sont utilis\u00e9s pour fixer les prot\u00e9ines et d’autres composants cellulaires.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbSolution de fixation pr\u00eate \u00e0 l’emploi\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]La solution de fixateur est g\u00e9n\u00e9ralement un m\u00e9lange de fixateur, de tampon et de divers additifs lorsque cela est sp\u00e9cifi\u00e9 dans votre protocole.<\/p>\n

      Pr\u00eat \u00e0 l’emploi : c’est-\u00e0-dire la concentration des fixateurs, la molarit\u00e9 et le type de tampon ajust\u00e9s au pH requis (selon votre protocole), en tenant compte de l’osmolarit\u00e9 finale de la solution, de quelques centaines de milliOsmoles pour les plantes, les invert\u00e9br\u00e9s et les mammif\u00e8res, \u00e0 un millier de milliOsmoles pour les esp\u00e8ces aquatiques ou marines.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbFixateurs ald\u00e9hydiques\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Les ald\u00e9hydes sont les fixateurs les plus couramment utilis\u00e9s. Ils sont utilis\u00e9s pour stabiliser les d\u00e9tails structurels les plus fins des cellules et des tissus avant leur examen par microscopie optique ou \u00e9lectronique.<\/p>\n

      Ils peuvent \u00eatre appel\u00e9s fixateurs primaires, tels que le ParaFormAldehyde (PFA), le Glutarald\u00e9hyde ou leur m\u00e9lange de Karnovsky.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbM\u00e9langes de fixateurs appel\u00e9s \u00ab\u00a0Karnovsky\u00ab\u00a0\u00ab\u00a0][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]M\u00e9langes contenant du paraformald\u00e9hyde (PFA) et du glutarald\u00e9hyde<\/p>\n

      La combinaison de paraformald\u00e9hyde et de glutarald\u00e9hyde comme fixateur pour la microscopie \u00e9lectronique b\u00e9n\u00e9ficie de la p\u00e9n\u00e9tration rapide de petites mol\u00e9cules HCHO, qui amorcent la stabilisation de la structure du tissu. Les oligom\u00e8res de glutarald\u00e9hyde, qui p\u00e9n\u00e8trent plus lentement, provoquent une r\u00e9ticulation rapide et compl\u00e8te. Ce m\u00e9lange est associ\u00e9 au nom de Morris J. Karnovsky de Boston. Il s’agit d’un exemple de grande innovation qui n’a \u00e9t\u00e9 publi\u00e9 que dans un r\u00e9sum\u00e9 non r\u00e9f\u00e9renc\u00e9 (Karnovsky, 1965).<\/p>\n

      Son m\u00e9lange original contenait 4 % de glutarald\u00e9hyde, une concentration plus \u00e9lev\u00e9e que celle que de nombreuses personnes souhaitaient utiliser (Hayat, 1981). Des appellations telles que \u00ab\u00a0Karnovsky demi-fort\u00a0\u00bb sont devenues courantes dans les ann\u00e9es 1960 et 1970. Le fixateur primaire a permis de r\u00e9soudre les observations de tissus difficiles \u00e0 pr\u00e9server en microscopie \u00e9lectronique. L’introduction du fixateur de Karnovsky a permis de r\u00e9soudre les observations de tissus difficiles \u00e0 pr\u00e9server en microscopie \u00e9lectronique, et ces fixateurs sont encore couramment utilis\u00e9s.<\/p>\n

      His original mixture contained 4% glutaraldehyde, which was a higher concentration than many people wanted to use (Hayat, 1981). Designations like \u00ab\u00a0half-strength Karnovsky\u00a0\u00bb became common parlance in the 1960s and 1970s. Primary fixative helped to resolve observations of tissues that were difficult to preserve em Electron microscopy . the introduction of Karnovsky fixative, and they are still commonly used.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbParaformald\u00e9hyde (PFA)\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Les ald\u00e9hydes sont les fixateurs les plus couramment utilis\u00e9s. Ils sont utilis\u00e9s pour stabiliser les d\u00e9tails structurels fins des cellules et des tissus avant la microscopie optique ou \u00e9lectronique.<\/p>\n

      Comme le glutarald\u00e9hyde, le paraformald\u00e9hyde est \u00e9galement couramment utilis\u00e9 et se d\u00e9polym\u00e9rise en formol lorsqu’il est chauff\u00e9, ce qui en fait \u00e9galement un fixateur efficace.[\/vc_column_text][\/toggle][toggle color=\u00a0\u00bbDefault\u00a0\u00bb heading_tag=\u00a0\u00bbdefault\u00a0\u00bb heading_tag_functionality=\u00a0\u00bbdefault\u00a0\u00bb title=\u00a0\u00bbGlutarald\u00e9hyde\u00a0\u00bb][vc_column_text text_direction=\u00a0\u00bbdefault\u00a0\u00bb]Pourquoi le glutarald\u00e9hyde est-il utilis\u00e9 en microscopie \u00e9lectronique ?<\/p>\n

      Le glutarald\u00e9hyde est l’un des fixateurs les plus fr\u00e9quemment utilis\u00e9s. Il r\u00e9agit rapidement avec les prot\u00e9ines et, en tant que diald\u00e9hyde, stabilise les structures par r\u00e9ticulation avant toute possibilit\u00e9 d’extraction du tampon.<\/p>\n

      Pour \u00e9viter la d\u00e9naturation du glutarald\u00e9hyde, il doit \u00eatre conserv\u00e9 \u00e0 4\u00b0C.[\/vc_column_text][\/toggle][\/toggles][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"

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