{"id":11083,"date":"2024-07-22T16:17:15","date_gmt":"2024-07-22T14:17:15","guid":{"rendered":"https:\/\/www.em-grade.com\/general-fr\/le-tampon-cacodylate-de-sodium-en-microscopie-electronique-bonnes-pratiques-et-alternatives\/"},"modified":"2025-05-16T11:29:15","modified_gmt":"2025-05-16T09:29:15","slug":"le-tampon-cacodylate-de-sodium-en-microscopie-electronique-bonnes-pratiques-et-alternatives","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/fr\/general-fr\/le-tampon-cacodylate-de-sodium-en-microscopie-electronique-bonnes-pratiques-et-alternatives\/","title":{"rendered":"Le tampon cacodylate de sodium en microscopie \u00e9lectronique\u202f: bonnes pratiques et alternatives"},"content":{"rendered":"

Tampon cacodylate de sodium<\/a>\u202f: une alternative efficace aux tampons phosphates<\/h2>\n

Le saviez-vous\u202f<\/strong>? Le tampon cacodylate de sodium (Na(CH\u2083)\u2082AsO\u2082\u00b73H\u2082O)<\/strong> est parmi les meilleures options pour la microscopie \u00e9lectronique et la pr\u00e9paration d\u2019\u00e9chantillons biologiques, surtout si vous souhaitez \u00e9viter l\u2019exc\u00e8s de phosphate\u202f!<\/p>\n

\n

Pourquoi choisir le tampon cacodylate de sodium\u202f?<\/h3>\n

Il excelle dans le maintien d\u2019un pH stable\u202f\u2014\u202fen particulier entre 5,0 et 7,4\u202f<\/strong>\u2014\u202fce qui rend les manipulations au laboratoire beaucoup plus simples et fiables.<\/p>\n

\n

Les avantages face aux tampons phosphates<\/h3>\n

D\u00e8s 1962, Sabatini et son \u00e9quipe ont propos\u00e9 le cacodylate de sodium pour la microscopie \u00e9lectronique. Pourquoi\u202f? Parce qu\u2019en utilisant d\u2019importantes quantit\u00e9s de phosphates (comme dans le tampon de S\u00f8rensen), on risque d\u2019ab\u00eemer les \u00e9chantillons\u202f: d\u00e9g\u00e2ts sur les mitochondries, alt\u00e9ration des organites, etc. Le cacodylate est nettement plus doux et plus s\u00fbr pour les pr\u00e9parations sensibles. <\/p>\n

\n

Compagnon id\u00e9al des fixateurs<\/h3>\n

Contrairement au Tris et \u00e0 d\u2019autres tampons amin\u00e9s, le cacodylate de sodium ne r\u00e9agit pas avec les fixateurs ald\u00e9hydes. R\u00e9sultat\u202f: vos \u00e9chantillons restent parfaitement pr\u00e9serv\u00e9s, fixation apr\u00e8s fixation. <\/p>\n

\n

Un petit plus unique\u202f?<\/h3>\n

Son efficacit\u00e9 viendrait plus de son effet inhibiteur sur le m\u00e9tabolisme de l\u2019ars\u00e9nate que de ses qualit\u00e9s tampon, selon certains chercheurs.<\/p>\n

\n

Vous voulez en savoir plus\u202f? \ud83d\udca1<\/h3>\n

Rendez-vous sur notre page FAQ <\/a>pour trouver toutes les r\u00e9ponses \u00e0 vos questions sur le cacodylate de sodium et les autres tampons utilis\u00e9s au laboratoire.<\/p>\n

\n

On reste en contact\u202f?<\/h3>\n

Des questions, des astuces, ou envie d\u2019am\u00e9liorer vos protocoles\u202f?
\nContactez directement notre \u00e9quipe\u202f:<\/a> nous sommes l\u00e0 pour vous aider\u202f!<\/p>\n","protected":false},"excerpt":{"rendered":"

Tampon cacodylate de sodium\u202f: une alternative efficace aux tampons phosphates Le saviez-vous\u202f? Le tampon cacodylate de sodium (Na(CH\u2083)\u2082AsO\u2082\u00b73H\u2082O) est parmi les meilleures options pour la microscopie \u00e9lectronique et la pr\u00e9paration…<\/p>\n","protected":false},"author":8,"featured_media":10472,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[159],"tags":[],"class_list":["post-11083","post","type-post","status-publish","format-standard","has-post-thumbnail","category-general-fr"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/11083","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/comments?post=11083"}],"version-history":[{"count":1,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/11083\/revisions"}],"predecessor-version":[{"id":11084,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/11083\/revisions\/11084"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media\/10472"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=11083"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/categories?post=11083"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/tags?post=11083"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}