{"id":4308,"date":"2023-10-24T12:14:18","date_gmt":"2023-10-24T10:14:18","guid":{"rendered":"https:\/\/www.em-grade.com\/general-fr\/les-tampons-en-microscopie\/"},"modified":"2024-06-12T11:31:18","modified_gmt":"2024-06-12T09:31:18","slug":"les-tampons-en-microscopie","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/fr\/tampons\/les-tampons-en-microscopie\/","title":{"rendered":"Les Tampons en microscopie"},"content":{"rendered":"<p><a href=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-scaled.jpg\"><img alt=\"\" loading=\"lazy\" decoding=\"async\" class=\"wp-image-1860 alignleft\" src=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-150x150.jpg\" alt=\"\" width=\"234\" height=\"234\" srcset=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-150x150.jpg 150w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-140x140.jpg 140w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-100x100.jpg 100w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-500x500.jpg 500w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-350x350.jpg 350w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-1000x1000.jpg 1000w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-800x800.jpg 800w\" sizes=\"auto, (max-width: 234px) 100vw, 234px\" \/><\/a>La pr\u00e9paration des \u00e9chantillons pour l&rsquo;observation en microscopie \u00e9lectronique, n\u00e9cessite les plus grandes pr\u00e9cautions lors de l&rsquo;utilisation de plusieurs solutions selon votre protocole \u00e0 diff\u00e9rentes \u00e9tapes, le respect \u00e9galement de diff\u00e9rents param\u00e8tres d\u00e9terminants et tr\u00e8s importants comme :<\/p>\n\r\n<ul class=\"eplus-lPd1kt wp-block-list\">&#13;\n<li>the <strong>pH \/Buffer,<\/strong><\/li>&#13;\n\r\n\r\n&#13;\n<li><strong>type de tampon<\/strong> en fonction de l&rsquo;\u00e9chantillon,<\/li>&#13;\n\r\n\r\n&#13;\n<li><strong>Osmolarit\u00e9<\/strong> du m\u00e9lange de fixation<\/li>&#13;\n\r\n\r\n&#13;\n<li><strong>temp\u00e9rature<\/strong><\/li>&#13;\n\r\n\r\n&#13;\n<li><strong>la dur\u00e9e de la fixation.<\/strong><\/li>&#13;\n<\/ul>\r\n<p>The pH of the different cell compartments can vary, so you need to adjust the pH of your buffer solutions according to your studies. In general, in more than 50% of the cases the buffers remain close from 7 to 7.4 A\u00a0<strong>buffer solution<\/strong>\u00a0maintains approximately the same\u00a0pH\u00a0despite the addition of small amounts of an\u00a0acid\u00a0or\u00a0base, or despite \u00a0dilution.<\/p>\r\n<ul>\r\n<li><strong><a href=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-scaled.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-1145 alignright\" src=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-150x150.jpg\" alt=\"PHEM Buffer\" width=\"272\" height=\"272\" srcset=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-150x150.jpg 150w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-140x140.jpg 140w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-100x100.jpg 100w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-500x500.jpg 500w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-350x350.jpg 350w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-1000x1000.jpg 1000w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-800x800.jpg 800w\" sizes=\"auto, (max-width: 272px) 100vw, 272px\" \/><\/a>phosphate or PB<\/strong>:\u00a0 A simple phosphate buffer is used ubiquitously in biological experiments, as it can be adapted to a variety of pH levels, including isotonic.\u00a0 This wide range is due to phosphoric acid having 3 dissociation constants<\/li>\r\n<li><strong>Tampon Phosphate Saline ou PBS <\/strong>a de nombreuses utilisations car elle est isotonique et non toxique pour la plupart des cellules. Ces utilisations comprennent la dilution de la substance et le rin\u00e7age des cellules. Le PBS avec EDTA est \u00e9galement utilis\u00e9 pour lib\u00e9rer les cellules attach\u00e9es et group\u00e9es. ce tampon est utilis\u00e9 en culture cellulaire sous atmosph\u00e8re contr\u00f4l\u00e9e dans des incubateurs \u00e0 5% de CO2<\/li>\r\n<li><strong>D-PBS<\/strong>: the <strong>Les DPBS<\/strong> ou <strong>Dulbecco\u2019s PBS <\/strong> est aussi connu sous le nom de CMF-PBS (calcium-magnesium free, or Ca Mg Free). <strong>C<\/strong>a <strong>M<\/strong>g <strong>F<\/strong>ree).<\/li>\r\n<li><strong>cacodylates : <\/strong> Le tampon cacodylate de sodium [Na (CH3) 2 AsO2 &#8211; 3H2O] est une alternative au tampon phosphate de S\u00f8rensen. Il poss\u00e8de un bon pouvoir tampon dans la plage de pH 5,0-7,4. Le cacodylate a \u00e9t\u00e9 introduit dans les applications de microscopie \u00e9lectronique par Sabatini et al. (1962) pour \u00e9viter d&rsquo;ajouter des phosphates suppl\u00e9mentaires aux pr\u00e9parations d&rsquo;\u00e9chantillons. Les mitochondries et autres organites peuvent \u00eatre endommag\u00e9s lorsqu&rsquo;ils sont expos\u00e9s aux fortes concentrations de phosphates pr\u00e9sentes dans les tampons de S\u00f8rensen. En outre, le cacodylate ne r\u00e9agit pas avec les fixateurs ald\u00e9hydiques comme le font les tampons contenant des amines (par exemple Tris). Son efficacit\u00e9 dans les solutions de liaison peut r\u00e9sulter de l&rsquo;effet inhibiteur du m\u00e9tabolisme de l&rsquo;ars\u00e9niate plut\u00f4t que d&rsquo;un pouvoir tampon particulier. Les tampons de Good, ou r\u00e9actifs tampons de Good, sont une liste de 20 produits chimiques formant des tampons, s\u00e9lectionn\u00e9s par Norman Good et son \u00e9quipe de 1966 \u00e0 1980 pour leur aptitude \u00e0 \u00eatre utilis\u00e9s en biochimie. Ces tampons sont appel\u00e9s \u00ab\u00a0tampons de Good\u00a0\u00bb. J&rsquo;en citerai quelques-uns utilis\u00e9s en microscopie \u00e9lectronique.<\/li>\r\n<\/ul>\r\n<ul>\r\n<li><strong>HEPES <\/strong><\/li>\r\n<li><strong>MOPS<\/strong><\/li>\r\n<li><strong>Tris , &#8230;<\/strong><\/li>\r\n<\/ul>\r\n","protected":false},"excerpt":{"rendered":"<p>La pr\u00e9paration des \u00e9chantillons pour l&rsquo;observation en microscopie \u00e9lectronique, n\u00e9cessite les plus grandes pr\u00e9cautions lors de l&rsquo;utilisation de plusieurs solutions selon votre protocole \u00e0 diff\u00e9rentes \u00e9tapes, le respect \u00e9galement de&#8230;<\/p>\n","protected":false},"author":8,"featured_media":3525,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[287],"tags":[267],"class_list":["post-4308","post","type-post","status-publish","format-standard","has-post-thumbnail","category-tampons","tag-tampons"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/4308","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/comments?post=4308"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/4308\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media\/3525"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=4308"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/categories?post=4308"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/tags?post=4308"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}