{"id":4384,"date":"2024-02-15T18:08:55","date_gmt":"2024-02-15T17:08:55","guid":{"rendered":"https:\/\/www.em-grade.com\/?p=4384"},"modified":"2024-03-26T11:30:06","modified_gmt":"2024-03-26T10:30:06","slug":"tampon-phem-application-recette","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/fr\/tampons\/tampon-phem-application-recette\/","title":{"rendered":"Tampon PHEM , application & recette."},"content":{"rendered":"

Recette de tampon PHEM<\/em><\/h2>\n

Le tampon PHEM a \u00e9t\u00e9 principalement utilis\u00e9 pour les tissus et les cultures cellulaires trait\u00e9es pour des \u00e9tudes immunocytochimiques. La plupart des antig\u00e8nes, en particulier ceux intracellulaires, se colorent mieux avec le PHEM que ceux trait\u00e9s avec le PBS ; ils ont \u00e9galement un effet plus limit\u00e9 sur les r\u00e9actions biochimiques et les enzymes. Une tr\u00e8s bonne pr\u00e9servation de l\u2019ultrastructure cellulaire, notamment des microtubules, est \u00e9galement un avantage de l\u2019utilisation du PHEM.<\/p>\n

\"\"<\/a><\/strong><\/p>\n

Les r\u00e9f\u00e9rences:<\/strong><\/p>\n

    \n
  1. Schliwa, Manfred et Jonathan Van Blerkom<\/strong><\/em>\u00a0. \u00ab\u00a0Interaction structurelle des composants du cytosquelette.\u00a0\u00bb Le Journal de biologie cellulaire 90.1 (1981) : 222-235.<\/li>\n
  2. Jacqueline Montanaro\u00a0:<\/em><\/strong>\u00a0Ultrastructure am\u00e9lior\u00e9e des invert\u00e9br\u00e9s marins \u00e0 l’aide de tampons non toxiques<\/li>\n<\/ol>\n

    <\/h4>\n

    Recette de pr\u00e9paration pour 500ml de Buffer PHEM \u00e0 0,2M<\/u><\/strong><\/h4>\n

    Dissoudre dans 400 ml d’eau distill\u00e9e cette poudre de r\u00e9actifs dans l’ordre suivant\u00a0:<\/em><\/p>\n\n\n\n\n\n\n\n\n
    Poudre de r\u00e9actif<\/strong><\/td>\nNum\u00e9ro CAS<\/strong><\/td>\npoids gr \/ Molarit\u00e9<\/strong><\/td>\nR\u00e9f. em-grade<\/strong><\/td>\n<\/tr>\n
    HEPES<\/strong><\/td>\n7365-45-9<\/td>\n0,598 gr. \/\u00a0 5mM<\/td>\nGP19<\/a><\/strong><\/td>\n<\/tr>\n
    EGTA<\/b><\/td>\n67-42-5<\/td>\n0,380 gr. \/\u00a0 2mM<\/td>\nGP27<\/strong><\/td>\n<\/tr>\n
    MgSO4 , 7H2O<\/strong><\/td>\n10034-99-8<\/td>\n0,098 gr. \/\u00a0 0,8mM<\/td>\nGP28<\/strong><\/td>\n<\/tr>\n
    Pipes<\/strong><\/td>\n5625-37-6<\/td>\n1,814 gr. \/\u00a0 12mM<\/td>\nGP21<\/a><\/strong><\/td>\n<\/tr>\n
    EAU<\/em>\u00a0D.<\/td>\nCompl\u00e9ter jusqu’\u00e0 500 ml de volume final et ajuster au pH souhait\u00e9 avec NaOH<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    Filtrer la solution \u00e0 0,22\u00b5.<\/em><\/p>\n

    Nous proposons \u00e9galement notre tampon PHEM pr\u00eat \u00e0 l’emploi disponible sur notre boutique :\u00a0PHEM Buffer<\/a>\u00a0.<\/h4>\n

    R\u00e9f. :\u00a0GT140000<\/span><\/a><\/h4>\n","protected":false},"excerpt":{"rendered":"

    Recette de tampon PHEM Le tampon PHEM a \u00e9t\u00e9 principalement utilis\u00e9 pour les tissus et les cultures cellulaires trait\u00e9es pour des \u00e9tudes immunocytochimiques. La plupart des antig\u00e8nes, en particulier ceux…<\/p>\n","protected":false},"author":8,"featured_media":3552,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[287],"tags":[291],"class_list":["post-4384","post","type-post","status-publish","format-standard","has-post-thumbnail","category-tampons","tag-phem-tampon"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/4384","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/comments?post=4384"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/4384\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media\/3552"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=4384"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/categories?post=4384"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/tags?post=4384"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}