{"id":4389,"date":"2024-02-15T18:18:18","date_gmt":"2024-02-15T17:18:18","guid":{"rendered":"https:\/\/www.em-grade.com\/?p=4389"},"modified":"2024-02-15T18:18:18","modified_gmt":"2024-02-15T17:18:18","slug":"comment-preparer-la-solution-de-coloration-ote-oolong","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/fr\/contrastants-em\/comment-preparer-la-solution-de-coloration-ote-oolong\/","title":{"rendered":"Comment pr\u00e9parer la solution de coloration OTE (Oolong) ?"},"content":{"rendered":"

Pr\u00e9paration et utilisation de l’OTE<\/span>\u00a0. (vid\u00e9o)<\/span><\/b><\/a><\/p>\n

protocole<\/span><\/strong><\/h3>\n

La pr\u00e9paration de la solution OTE est similaire \u00e0 l’infusion d’eau de th\u00e9 \u00e0 70\u00b0C pas plus.<\/span><\/p>\n

\u2013 A l\u2019aide d\u2019un agitateur magn\u00e9tique chauffant r\u00e9gl\u00e9 \u00e0 70\u00b0C, dissolvez votre poudre d\u2019OTE \u00e0 une concentration de 0,2% maximum (car la saturation est atteinte \u00e0 0,3%)<\/span><\/p>\n

\u2013 Apr\u00e8s dissolution compl\u00e8te (atteinte en 15 \u00e0 30 minutes sous agitation \u00e0 70\u00b0C),<\/span><\/p>\n

    \n
  1. Filtrez votre solution d’extrait OTE \u00e0 0,45\u00b5 ou mieux \u00e0 0,22\u00b5<\/span><\/li>\n
  2. A conserver dans un petit tube Eppendorf rempli au maximum pour r\u00e9duire l’air contenant de l’oxyg\u00e8ne ou dans un\u00a0<\/span>flacon AirLess<\/span><\/a>\u00a0.<\/span><\/li>\n
  3. Conserver \u00e0 l’obscurit\u00e9 \u00e0 4 \u00b0C (lors de la manipulation, laissez toujours vos solutions revenir \u00e0 TA)<\/span><\/li>\n
  4. Contrastez vos grilles avec OTE pendant 15 \u00e0 20 minutes, obscurit\u00e9, RT<\/span><\/li>\n
  5. Egoutter simplement ne pas rincer ou laver tr\u00e8s bri\u00e8vement 1 \u00e0 2 secondes, apr\u00e8s s\u00e9chage<\/span><\/li>\n
  6. Post-coloration 1 \u00e0 2 minutes avec du citrate de plomb selon Reynolds (Ref #11300)<\/span><\/li>\n
  7. \u00c9goutter, rincer bri\u00e8vement, s\u00e9cher et pr\u00eat \u00e0 observer.<\/span><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    Pr\u00e9paration et utilisation de l’OTE\u00a0. (vid\u00e9o) protocole La pr\u00e9paration de la solution OTE est similaire \u00e0 l’infusion d’eau de th\u00e9 \u00e0 70\u00b0C pas plus. \u2013 A l\u2019aide d\u2019un agitateur magn\u00e9tique…<\/p>\n","protected":false},"author":8,"featured_media":3526,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[289],"tags":[293],"class_list":["post-4389","post","type-post","status-publish","format-standard","has-post-thumbnail","category-contrastants-em","tag-ote-stain-em"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/4389","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/comments?post=4389"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/4389\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media\/3526"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=4389"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/categories?post=4389"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/tags?post=4389"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}