{"id":4392,"date":"2024-02-15T18:22:35","date_gmt":"2024-02-15T17:22:35","guid":{"rendered":"https:\/\/www.em-grade.com\/?p=4392"},"modified":"2024-02-15T18:22:35","modified_gmt":"2024-02-15T17:22:35","slug":"phem-buffer-le-meilleur-tampon-pour-la-culture-cellulaire-par-immunofluorescence","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/fr\/tampons\/phem-buffer-le-meilleur-tampon-pour-la-culture-cellulaire-par-immunofluorescence\/","title":{"rendered":"PHEM BUFFER , le meilleur tampon pour la culture cellulaire par immunofluorescence."},"content":{"rendered":"

Le tampon PHEM<\/strong>\u00a0a \u00e9t\u00e9 d\u00e9velopp\u00e9 pour pr\u00e9server les microtubules et est utilis\u00e9 pour pr\u00e9server\u00a0<\/span>la structure cellulaire lors de l’immunocoloration<\/span>\u00a0\u00a0<\/span><\/p>\n

R\u00e9f\u00e9rences<\/span><\/strong>\u00a0: Schliwa, Manfred et Jonathan Van Blerkom. \u00ab\u00a0Interaction structurelle des composants du cytosquelette.\u00a0\u00bb Le Journal de biologie cellulaire 90.1 (1981) : 222-235.\u00a0<\/span><\/em><\/p>\n

Le tampon PHEM est recommand\u00e9 dans les \u00e9tudes immunocytochimiques et d\u2019immunocoloration pour les cultures de tissus et de cellules.<\/span><\/p>\n

\"\"<\/a>\"\"<\/a><\/p>\n

Des travaux en laboratoire ont montr\u00e9 que le tampon PHEM permet une meilleure d\u00e9tection des antig\u00e8nes intracellulaires et moins d’impact sur les r\u00e9actions enzymatiques et biochimiques,<\/span>
\ntandis que le PBS est plus sp\u00e9cifique aux antig\u00e8nes membranaires extracellulaires, pour cette raison le PHEM est pr\u00e9f\u00e9r\u00e9 lors des marquages \u200b\u200bpar fluorescence ;<\/span>
\nL\u2019utilisation du PHEM assure \u00e9galement une excellente pr\u00e9servation de l\u2019ultrastructure cellulaire, notamment des microtubules. Le tampon connu sous le nom de CSK, un d\u00e9riv\u00e9 de la formulation PHEM est appel\u00e9\u00a0Cyto\u00a0<\/span>SK\u00a0<\/span><\/strong>eletal<\/span><\/strong>\u00a0(CSK). Le tampon semble am\u00e9liorer le marquage des microtubules.<\/span><\/p>\n

 <\/p>\n

plus d’informations<\/span><\/strong>\u00a0Recette du tampon PHEM<\/span><\/a><\/h3>\n","protected":false},"excerpt":{"rendered":"

Le tampon PHEM\u00a0a \u00e9t\u00e9 d\u00e9velopp\u00e9 pour pr\u00e9server les microtubules et est utilis\u00e9 pour pr\u00e9server\u00a0la structure cellulaire lors de l’immunocoloration\u00a0\u00a0 R\u00e9f\u00e9rences\u00a0: Schliwa, Manfred et Jonathan Van Blerkom. \u00ab\u00a0Interaction structurelle des composants…<\/p>\n","protected":false},"author":8,"featured_media":3735,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[287],"tags":[291],"class_list":["post-4392","post","type-post","status-publish","format-standard","has-post-thumbnail","category-tampons","tag-phem-tampon"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/4392","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/comments?post=4392"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/4392\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media\/3735"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=4392"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/categories?post=4392"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/tags?post=4392"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}