{"id":6887,"date":"2024-02-25T11:32:51","date_gmt":"2024-02-25T10:32:51","guid":{"rendered":"https:\/\/www.em-grade.com\/general-fr\/recette-preparation-pbs-phosphate-buffer-saline-1x-ph-7-4\/"},"modified":"2025-05-16T12:39:16","modified_gmt":"2025-05-16T10:39:16","slug":"recette-preparation-pbs-phosphate-buffer-saline-1x-ph-7-4","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/fr\/tampons\/recette-preparation-pbs-phosphate-buffer-saline-1x-ph-7-4\/","title":{"rendered":"Recette \/ Pr\u00e9paration : PBS (Phosphate Buffer Saline) 1X, pH 7.4."},"content":{"rendered":"

Pr\u00e9paration de 500 mL de PBS (Phosphate Buffer Saline) 1X, pH 7.4<\/h3>\n

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Composition pour 500\u202fmL de PBS 1X (pH 7,4)<\/h4>\n\n\n\n\n\n\n\n\n\n
Composant<\/th>\nQuantit\u00e9<\/th>\nConcentration finale<\/th>\nMasse molaire (g\/mol)<\/th>\n<\/tr>\n<\/thead>\n
Chlorure de sodium (NaCl)<\/td>\n4,0 g<\/td>\n0,137 M<\/td>\n58,44<\/td>\n<\/tr>\n
Chlorure de potassium (KCl)<\/td>\n0,1 g<\/td>\n0,0027 M<\/td>\n74,55<\/td>\n<\/tr>\n
Phosphate de sodium dibasique (Na\u2082HPO\u2084)<\/td>\n0,72 g<\/td>\n0,01 M<\/td>\n141,96<\/td>\n<\/tr>\n
Phosphate de potassium monobasique (KH\u2082PO\u2084)<\/td>\n0,1225 g<\/td>\n0,0018 M<\/td>\n136,09<\/td>\n<\/tr>\n
Eau distill\u00e9e<\/td>\nq.s.p 500 mL<\/td>\n\u2014<\/td>\n\u2014<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
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\u00c9tapes de pr\u00e9paration du PBS 1X (500\u202fmL)<\/h3>\n
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  1. Verser environ 450\u202fmL d\u2019eau distill\u00e9e dans un b\u00e9cher ou une fiole jaug\u00e9e propre.<\/li>\n
  2. Ajouter 4,0\u202fg de chlorure de sodium (NaCl). Agiter pour dissoudre.<\/li>\n
  3. Ajouter 0,1\u202fg de chlorure de potassium (KCl) \u00e0 la solution.<\/li>\n
  4. Ajouter 0,72\u202fg de phosphate de sodium dibasique (Na\u2082HPO\u2084).<\/li>\n
  5. Ajouter 0,1225\u202fg de phosphate de potassium monobasique (KH\u2082PO\u2084).<\/li>\n
  6. M\u00e9langer attentivement jusqu’\u00e0 dissolution compl\u00e8te de tous les compos\u00e9s.<\/li>\n
  7. Contr\u00f4ler le pH <\/a>et ajuster \u00e0 7,4 \u00e0 l\u2019aide de HCl ou NaOH si besoin.<\/li>\n
  8. Compl\u00e9ter le volume \u00e0 500\u202fmL avec de l\u2019eau distill\u00e9e.<\/li>\n
  9. (Optionnel) St\u00e9riliser la solution par filtration (0,22\u202f\u00b5m) ou en autoclave.<\/li>\n<\/ol>\n
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    Conseils Pratiques<\/h3>\n