{"id":6889,"date":"2024-02-21T16:58:03","date_gmt":"2024-02-21T15:58:03","guid":{"rendered":"https:\/\/www.em-grade.com\/general-fr\/la-coloration77-a-positive-staining-ultrathins-sections-on-tem-grids\/"},"modified":"2024-03-08T14:51:38","modified_gmt":"2024-03-08T13:51:38","slug":"la-coloration77-a-positive-staining-ultrathins-sections-on-tem-grids","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/fr\/contrastants-em\/la-coloration77-a-positive-staining-ultrathins-sections-on-tem-grids\/","title":{"rendered":"Stain77 : contraste positif des coupes ultrafines MET ."},"content":{"rendered":"

Stain77, nouvel agent de contraste en microscopie \u00e9lectronique.
\nStain77 est un produit de contraste doublement efficace<\/strong> sur les coupes ultrafines et dans la coloration n\u00e9gative des particules isol\u00e9es en microscopie \u00e9lectronique.<\/p>\n

\"La<\/a>Comme Uranyless, n\u00e9cessitant un contact pendant une minute avec les sections d’ultrathins sur une grille, puis un simple \u00e9gouttage sur papier filtre suivi d’un second contact sur une goutte de citrate de plomb pendant 20 \u00e0 30 secondes finalis\u00e9 par un court lavage \u00e0 l’eau distill\u00e9e.<\/p>\n

Il donne un contraste tr\u00e8s doux et met parfaitement en \u00e9vidence les diff\u00e9rents organites cellulaires. Combin\u00e9 \u00e0 un post-traitement au citrate de plomb, il est possible de d\u00e9limiter les compartiments cellulaires en marquant parfaitement les cyto-membranes.<\/p>\n

\"\"<\/a> \"\"<\/a>\"\"<\/a>La toxicit\u00e9 est quasi nulle<\/strong> et la coloration peut se faire sur le bord de la paillasse, c’est un sel de Lithium associ\u00e9 \u00e0 une tr\u00e8s faible concentration de Tungst\u00e8ne, son pH est proche de 7.<\/p>\n

Il est facile \u00e0 mettre en \u0153uvre et stable dans le temps. Il peut \u00eatre utilis\u00e9 comme alternative \u00e0 l’UranyLess aqueux lorsque ce dernier n’est pas concluant pour votre application.<\/p>\n

Nous continuons \u00e0 d\u00e9velopper de nouveaux produits de contraste car, comme il n’existe pas de fixateur universel, le contraste peut toujours \u00eatre am\u00e9lior\u00e9 pour mieux mettre en \u00e9vidence toutes les microstructures cellulaires sur diff\u00e9rentes surfaces.<\/p>\n","protected":false},"excerpt":{"rendered":"

Stain77, nouvel agent de contraste en microscopie \u00e9lectronique. Stain77 est un produit de contraste doublement efficace sur les coupes ultrafines et dans la coloration n\u00e9gative des particules isol\u00e9es en microscopie…<\/p>\n","protected":false},"author":8,"featured_media":4445,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[289],"tags":[636],"class_list":["post-6889","post","type-post","status-publish","format-standard","has-post-thumbnail","category-contrastants-em","tag-stain77-fr"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/6889","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/comments?post=6889"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/6889\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=6889"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/categories?post=6889"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/tags?post=6889"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}