{"id":7248,"date":"2023-11-10T11:14:41","date_gmt":"2023-11-10T10:14:41","guid":{"rendered":"https:\/\/www.em-grade.com\/general-fr\/le-tampon-imidazole-permet-la-retention-des-lipides\/"},"modified":"2023-11-10T11:14:41","modified_gmt":"2023-11-10T10:14:41","slug":"le-tampon-imidazole-permet-la-retention-des-lipides","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/fr\/tampons\/le-tampon-imidazole-permet-la-retention-des-lipides\/","title":{"rendered":"Le tampon imidazole permet la r\u00e9tention des lipides."},"content":{"rendered":"

Le tampon imidazole permet de conserver les lipides intracellulaires les plus difficiles \u00e0 pr\u00e9server<\/strong>.<\/p>\n

Le tampon imidazole permet la r\u00e9tention des lipides.<\/u><\/strong> associ\u00e9 \u00e0 l’osmium lors de la fixation en tant que tampon \u00e0 0,1M<\/p>\n

Voici un protocole standard<\/p>\n

    \n
  1. Glutarald\u00e9hyde ‘2 \u00e0 2,5% dans un tampon imidazole 0,1M<\/strong> pH7,5 – RT<\/li>\n
  2. Laver dans un tampon imidazole 0,2M<\/strong> pH7,5 (2 bains 3 minutes) \u00e0 temp\u00e9rature ambiante.<\/li>\n
  3. Osmium 1% \u00e0 2% dans un tampon imidazole<\/strong> 0,1 pH 7,5, 30 min – RT<\/li>\n
  4. Dernier lavage identique au point 2 (lavage dans un tampon imidazole 0,2M pH7,5 (2 bains au maximum, 3 minutes) \u00e0 temp\u00e9rature ambiante.<\/li>\n<\/ol>\n

    Remplacez vos tampons Sorensen PBS ou cacodylate habituels par des tampons imidazole, lorsque votre \u00e9tude accorde de l’importance aux inclusions lipidiques.<\/p>\n

    Note importante : si vous voulez pr\u00e9server le contenu des cellules fix\u00e9es, \u00e9vitez de trop laver, 1 lavage suffit, si les lipides simples sont les plus difficiles \u00e0 pr\u00e9server, le lavage devrait m\u00eame \u00eatre \u00e9vit\u00e9.<\/p>\n

    \"\" <\/a> \"conservation<\/a><\/p>\n

    cellule h\u00e9patocytaire<\/p>\n","protected":false},"excerpt":{"rendered":"

    Le tampon imidazole permet de conserver les lipides intracellulaires les plus difficiles \u00e0 pr\u00e9server. Le tampon imidazole permet la r\u00e9tention des lipides. associ\u00e9 \u00e0 l’osmium lors de la fixation en…<\/p>\n","protected":false},"author":8,"featured_media":3514,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[287],"tags":[672,671],"class_list":["post-7248","post","type-post","status-publish","format-standard","has-post-thumbnail","category-tampons","tag-lipides","tag-tampon-imidazole"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/7248","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/comments?post=7248"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/7248\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media\/3514"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=7248"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/categories?post=7248"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/tags?post=7248"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}