{"id":7332,"date":"2023-11-17T11:33:02","date_gmt":"2023-11-17T10:33:02","guid":{"rendered":"https:\/\/www.em-grade.com\/general-fr\/em-stain-ote-oolong-tea-utiliser-la-coloration-des-coupes-ultrafines\/"},"modified":"2023-11-17T11:33:02","modified_gmt":"2023-11-17T10:33:02","slug":"em-stain-ote-oolong-tea-utiliser-la-coloration-des-coupes-ultrafines","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/fr\/contrastants-em\/em-stain-ote-oolong-tea-utiliser-la-coloration-des-coupes-ultrafines\/","title":{"rendered":"EM Stain OTE (OOLONG TEA) utiliser la coloration des coupes ultrafines."},"content":{"rendered":"

Pr\u00e9paration et utilisation de l’O.T.E.<\/p>\n

La pr\u00e9paration de la solution OTE est similaire \u00e0 l’infusion d’eau de th\u00e9 \u00e0 70\u00b0C ou de tampon 0,1M.(*)<\/p>\n

– A l’aide d’un agitateur magn\u00e9tique chauffant r\u00e9gl\u00e9 \u00e0 70\u00b0C, dissolvez votre poudre d’OTE \u00e0 une concentration de 0,2% (car la saturation est atteinte \u00e0 0,3%).<\/p>\n

– Apr\u00e8s dissolution compl\u00e8te (15 \u00e0 30 minutes sous agitation \u00e0 70\u00b0C),<\/p>\n

    \n
  1. Filtrez votre solution d’extrait OTE \u00e0 0,45\u00b5.<\/li>\n
  2. Conserver dans un petit tube Eppendorf rempli au maximum pour r\u00e9duire l’air contenant de l’oxyg\u00e8ne ou dans un flacon AirLess.<\/li>\n
  3. Conserver dans l’obscurit\u00e9 \u00e0 4 \u00b0C (lors de la manipulation, toujours laisser les solutions revenir \u00e0 la temp\u00e9rature de r\u00e9f\u00e9rence).<\/li>\n
  4. Contraster vos grilles avec l’OTE pendant 15 \u00e0 25 minutes, dans l’obscurit\u00e9, RT<\/li>\n
  5. Egoutter simplement et non pas rincer<\/li>\n
  6. Post-tache 1 \u00e0 2 minutes avec du citrate de plomb selon Reynolds (Ref # 11300 de Delta Microscopies)<\/li>\n
  7. Egouttez, rincez bri\u00e8vement, s\u00e9chez et soyez pr\u00eats \u00e0 regarder.<\/li>\n<\/ol>\n

    *Note : la solution peut \u00eatre conserv\u00e9e pendant une semaine au maximum, mais plus longtemps dans une solution tampon de cacodylate, dans un flacon sans air (pas de croissance bact\u00e9rienne).<\/p>\n

    \"\" <\/a>\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

    Pr\u00e9paration et utilisation de l’O.T.E. La pr\u00e9paration de la solution OTE est similaire \u00e0 l’infusion d’eau de th\u00e9 \u00e0 70\u00b0C ou de tampon 0,1M.(*) – A l’aide d’un agitateur magn\u00e9tique…<\/p>\n","protected":false},"author":8,"featured_media":3518,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[289],"tags":[686,294],"class_list":["post-7332","post","type-post","status-publish","format-standard","has-post-thumbnail","category-contrastants-em","tag-extrait-de-the-oolong","tag-ote-fr"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/7332","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/comments?post=7332"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/7332\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media\/3518"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=7332"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/categories?post=7332"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/tags?post=7332"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}