{"id":7344,"date":"2023-11-23T11:42:59","date_gmt":"2023-11-23T10:42:59","guid":{"rendered":"https:\/\/www.em-grade.com\/general-fr\/comment-preparer-la-solution-de-coloration-o-t-e-oolong\/"},"modified":"2023-11-23T11:42:59","modified_gmt":"2023-11-23T10:42:59","slug":"comment-preparer-la-solution-de-coloration-o-t-e-oolong","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/fr\/contrastants-em\/comment-preparer-la-solution-de-coloration-o-t-e-oolong\/","title":{"rendered":"Comment pr\u00e9parer la solution de coloration O.T.E. (Oolong) ?"},"content":{"rendered":"<p><iframe loading=\"lazy\" title=\"OTE EM staining ( Oolong Tea Extract ) . EM Stain\" width=\"1080\" height=\"608\" src=\"https:\/\/www.youtube.com\/embed\/i7lZhu4Pf9o?start=104&#038;feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" allowfullscreen><\/iframe><\/p>\n<h3><strong><span style=\"color: #000000;\">Protocole<\/span><\/strong><\/h3>\n<p>La pr\u00e9paration de la solution OTE est similaire \u00e0 l&rsquo;infusion d&rsquo;eau de th\u00e9 \u00e0 70\u00b0C pas plus.<\/p>\n<p>&#8211; A l&rsquo;aide d&rsquo;un agitateur magn\u00e9tique chauffant r\u00e9gl\u00e9 \u00e0 70\u00b0C, dissolvez votre poudre d&rsquo;OTE \u00e0 une concentration de 0,2% au maximum (car la saturation est atteinte \u00e0 0,3%).<\/p>\n<p>&#8211; Apr\u00e8s dissolution compl\u00e8te (atteinte en 15 \u00e0 30 minutes sous agitation \u00e0 70\u00b0C),<\/p>\n<ol>\n<li>Filtrez votre solution d&rsquo;extraction OTE \u00e0 0.45\u00b5 ou mieux \u00e0 0.22\u00b5<\/li>\n<li>Conserver dans un petit tube Eppendorf rempli au maximum pour r\u00e9duire l&rsquo;air contenant de l&rsquo;oxyg\u00e8ne ou dans un <a href=\"https:\/\/www.em-grade.com\/microscopy-advice\/bottles-with-airless-system\/\">flacon AirLess<\/a>.<\/li>\n<li>Conserver dans l&rsquo;obscurit\u00e9 \u00e0 4 \u00b0C (lors de la manipulation, toujours laisser les solutions revenir \u00e0 la temp\u00e9rature de r\u00e9f\u00e9rence).<\/li>\n<li>Contraster vos grilles avec l&rsquo;OTE pendant 15 \u00e0 20 minutes, dans l&rsquo;obscurit\u00e9, RT<\/li>\n<li>Vidange simplement sans rin\u00e7age ou lavage tr\u00e8s court 1 \u00e0 2 secondes, apr\u00e8s s\u00e9chage<\/li>\n<li>Post-tache 1 \u00e0 2 minutes avec du citrate de plomb selon Reynolds (Ref # 11300)<\/li>\n<li>\u00c9gouttez, rincez bri\u00e8vement, s\u00e9chez et soyez pr\u00eats \u00e0 observer.<\/li>\n<\/ol>\n<p><b>   <\/b><\/p>\n<p>Poudre OTE , 10gr seulement par <a href=\"https:\/\/www.em-grade.com\/product\/oolong-tea-extract-ote-powder\/\"><strong>EM-grade.com<\/strong><\/a><\/p>\n<p><a href=\"https:\/\/www.em-grade.com\/product\/oolong-tea-extract-ote-powder\/\"><span style=\"color: #ff0000;\"><b>Code produit # GP18-10<\/b><\/span><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Protocole La pr\u00e9paration de la solution OTE est similaire \u00e0 l&rsquo;infusion d&rsquo;eau de th\u00e9 \u00e0 70\u00b0C pas plus. &#8211; A l&rsquo;aide d&rsquo;un agitateur magn\u00e9tique chauffant r\u00e9gl\u00e9 \u00e0 70\u00b0C, dissolvez votre&#8230;<\/p>\n","protected":false},"author":8,"featured_media":3526,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[289],"tags":[701],"class_list":["post-7344","post","type-post","status-publish","format-standard","has-post-thumbnail","category-contrastants-em","tag-o-t-e-fr"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/7344","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/comments?post=7344"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/7344\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media\/3526"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=7344"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/categories?post=7344"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/tags?post=7344"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}