<\/p>\n
Contraste d’\u00e9chantillons biologiques (h\u00e9patocytes, Thrombocytes) \u00e0 l’aide d’une solution\u00a0 \u00e0 base d’ O.T.E avant inclusion sur culot de cellules.<\/strong><\/h3>\n <\/p>\n
\u00a0protocole<\/em> :<\/h3>\n–Pr\u00e9paration pr\u00e9alable de l\u2019OTE<\/a> \u00e0 0.2% (solution pr\u00e9alablement aliquot\u00e9e et congel\u00e9e)<\/p>\n\n- Fixation glutarald\u00e9hyde 1% en tampon cacodylate de Na 0.1M, pH 7.4 2h \u00e0 4 degr\u00e9<\/li>\n
- 3 rin\u00e7ages en cacodylate de Na 0.1M de quelques heures \u00e0 1 nuit \u00e0 +4\u00b0C<\/li>\n
- Post fixation\u00a0: T\u00e9troxyde d\u2019osmium 1% +1.5% de ferrocyanure de potassium dans du tampon cacodylate dans du tampon cacodylate de Na 0.1M, pH 7.4<\/li>\n
- Rin\u00e7ages en tampon cacodylate de Na 0.1M, pH 7.4<\/li>\n
- Contraste OTE 0.2% dans du cacodylate 1h TA<\/li>\n
- Rin\u00e7ages \u00e0 l\u2019eau distill\u00e9e.<\/li>\n
- D\u00e9shydratation\u00a0: \u00e9thanol 30\u00b0 (5min), 50\u00b0(5min), 70\u00b0(5min), 95\u00b0(5min), 100\u00b0(3x10min)<\/li>\n
- Substitution : R\u00e9sine Epon 1v<\/em>. \/ Ethanol absolu 1v<\/em>.<\/li>\n
- Impr\u00e9gnation en r\u00e9sine pure Epon sous vide<\/li>\n
- Inclusion avec Epon +DMP30. Polym\u00e9risation \u00e0 60\u00b0 pendant 48h<\/li>\n
- Contraste des coupes ultrafine 5min au citrate de plomb<\/li>\n
- Rin\u00e7age \u00e0 l\u2019eau<\/li>\n<\/ul>\n
Conclusion : ce contrastant \u00e0 base d’extrait de Th\u00e9 d’Oolong (O.T.E)<\/a> permet un belle visualisation des constituants intracytoplasmiques et repr\u00e9sente un bon substitut \u00e0 l\u2019ac\u00e9tate d\u2019uranyle .<\/p>\nNotre exp\u00e9rience de contraste avec l’utilisation de l’OTE sur deux types de tissu foie et Thrombocytes est tr\u00e8s concluante avec une bonne visualisation des diff\u00e9rents compartiments et organites cellulaire. l’OTE \u00ab\u00a0safe stain\u00a0\u00bb<\/strong><\/em> nous a permis d’\u00e9carter des contrastants toxique comme l’ac\u00e9tate d’uranyle.<\/p>\n <\/p>\n
\u00a0R\u00e9sultats\u00a0:<\/h3>\n
Photo1 : Visualisation des contacts entre les mitochondries et les r\u00e9ticulums dans un h\u00e9patocyte. ![\"\"](\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/03\/OTE-hepatocyte-Cicle-lyon-150x150.jpg\")
<\/a><\/strong><\/span><\/p>\n\n- Gly<\/strong> : Grains de glycog\u00e8ne , Mit<\/strong> : Mitochondrie,\u00a0REG<\/strong> : R\u00e9ticulum endoplasmique rugueux,\u00a0Lip<\/strong>\u00a0: Gouttelette lipidique<\/li>\n
- Cr\u00e9dit photo<\/strong> : Jennifer Rieusset-\u00a0CARMEN – INSERM U1060 \u2013Lyon et CIQLE (Centre d\u2019Imagerie Quantitative Lyon Est)<\/li>\n
- \u00c9quipement:<\/strong> microscope \u00e9lectronique \u00e0 transmission\u00a0JEOL EUROPE<\/a>\u00a0JEM 1400, cam\u00e9ra\u00a0Gatan Inc.<\/a>\u00a0Orius600<\/li>\n
- <\/li>\n<\/ul>\n
<\/p>\n
cliquer sur image pour agrandir la photo.<\/em><\/span><\/p>\n <\/p>\n
<\/p>\n
Photo2 : Comptage des granules alpha dans les plaquettes sanguines de patient.<\/strong><\/span><\/p>\n\n![\"\"](\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/03\/OTE-hepatocyte-CiQle-lyon-150x150.jpg\")
<\/a>DTS<\/strong>: Dense tubular syst\u00e8me,\u00a0 Gly<\/strong>: Glycogen,\u00a0 OCS<\/strong>: Open canalicular syst\u00e8me,\u00a0 \u03bb-G:<\/strong> Alpha granules<\/li>\n- Cr\u00e9dit photo<\/strong> : Ana\u00eblle De Wreede-\u00a0Hospices Civils de Lyon – HCL<\/a>\u00a0– Laboratoire d\u2019H\u00e9mostase et CIQLE (Centre d\u2019Imagerie Quantitative Lyon Est)<\/li>\n
- \u00c9quipement<\/strong>: microscope \u00e9lectronique \u00e0 transmission\u00a0JEOL EUROPE<\/a>\u00a0JEM 1400, cam\u00e9ra\u00a0Gatan Inc.<\/a>\u00a0Orius600<\/li>\n<\/ul>\n
<\/p>\n
cliquer sur image pour agrandir la photo.<\/strong><\/em><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"On vous fait partager une exp\u00e9rience d’utilisation d’un contrastant non toxique comme l’OTE , sous la direction d’Elisabeth ERRAZURIZ-CERDA du\u00a0CIQLE (Centre d’ Imagerie Quantitative Lyon Est). Contraste d’\u00e9chantillons biologiques…<\/p>\n","protected":false},"author":8,"featured_media":7786,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[289],"tags":[],"class_list":["post-7761","post","type-post","status-publish","format-standard","has-post-thumbnail","category-contrastants-em"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/7761","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/comments?post=7761"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/7761\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media\/7786"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=7761"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/categories?post=7761"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/tags?post=7761"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
–Pr\u00e9paration pr\u00e9alable de l\u2019OTE<\/a> \u00e0 0.2% (solution pr\u00e9alablement aliquot\u00e9e et congel\u00e9e)<\/p>\n Conclusion : ce contrastant \u00e0 base d’extrait de Th\u00e9 d’Oolong (O.T.E)<\/a> permet un belle visualisation des constituants intracytoplasmiques et repr\u00e9sente un bon substitut \u00e0 l\u2019ac\u00e9tate d\u2019uranyle .<\/p>\n Notre exp\u00e9rience de contraste avec l’utilisation de l’OTE sur deux types de tissu foie et Thrombocytes est tr\u00e8s concluante avec une bonne visualisation des diff\u00e9rents compartiments et organites cellulaire. l’OTE \u00ab\u00a0safe stain\u00a0\u00bb<\/strong><\/em> nous a permis d’\u00e9carter des contrastants toxique comme l’ac\u00e9tate d’uranyle.<\/p>\n <\/p>\n Photo1 : Visualisation des contacts entre les mitochondries et les r\u00e9ticulums dans un h\u00e9patocyte. <\/p>\n cliquer sur image pour agrandir la photo.<\/em><\/span><\/p>\n <\/p>\n <\/p>\n Photo2 : Comptage des granules alpha dans les plaquettes sanguines de patient.<\/strong><\/span><\/p>\n <\/p>\n cliquer sur image pour agrandir la photo.<\/strong><\/em><\/span><\/p>\n","protected":false},"excerpt":{"rendered":" On vous fait partager une exp\u00e9rience d’utilisation d’un contrastant non toxique comme l’OTE , sous la direction d’Elisabeth ERRAZURIZ-CERDA du\u00a0CIQLE (Centre d’ Imagerie Quantitative Lyon Est). Contraste d’\u00e9chantillons biologiques…<\/p>\n","protected":false},"author":8,"featured_media":7786,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[289],"tags":[],"class_list":["post-7761","post","type-post","status-publish","format-standard","has-post-thumbnail","category-contrastants-em"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/7761","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/comments?post=7761"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/posts\/7761\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media\/7786"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=7761"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/categories?post=7761"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/tags?post=7761"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}\n
\u00a0R\u00e9sultats\u00a0:<\/h3>\n
<\/a><\/strong><\/span><\/p>\n\n
\n
<\/a>DTS<\/strong>: Dense tubular syst\u00e8me,\u00a0 Gly<\/strong>: Glycogen,\u00a0 OCS<\/strong>: Open canalicular syst\u00e8me,\u00a0 \u03bb-G:<\/strong> Alpha granules<\/li>\n