{"id":11402,"date":"2023-12-04T16:34:56","date_gmt":"2023-12-04T15:34:56","guid":{"rendered":"https:\/\/www.em-grade.com\/?post_type=product&#038;p=11402"},"modified":"2025-11-21T17:07:54","modified_gmt":"2025-11-21T16:07:54","slug":"tampon-phosphate-salin-pbs","status":"publish","type":"product","link":"https:\/\/www.em-grade.com\/fr\/produit\/tampon-phosphate-salin-pbs\/","title":{"rendered":"Tampon Phosphate Salin (PBS)"},"content":{"rendered":"<h3 data-start=\"233\" data-end=\"263\"><strong data-start=\"237\" data-end=\"263\">Description du produit<\/strong><\/h3>\n<p data-start=\"264\" data-end=\"652\"><a href=\"https:\/\/www.em-grade.com\/fr\/tampons\/le-tampon-p-b-s\/\">Le PBS (Phosphate-Buffered Saline)<\/a> est une solution tampon couramment utilis\u00e9e en biochimie. Il s\u2019agit d\u2019une solution physiologique contenant du chlorure de sodium, du phosphate disodique, du phosphate monopotassique et une faible quantit\u00e9 de chlorure de potassium.<br data-start=\"529\" data-end=\"532\" \/>Les concentrations en sels sont g\u00e9n\u00e9ralement \u00e9quivalentes \u00e0 celles du milieu physiologique humain (solution isotonique).<\/p>\n<h3 data-start=\"654\" data-end=\"674\"><strong data-start=\"658\" data-end=\"674\">Applications<\/strong><\/h3>\n<p data-start=\"675\" data-end=\"918\">Cette solution tampon est principalement utilis\u00e9e pour le rin\u00e7age des cellules afin d\u2019\u00e9liminer toute trace de milieu avant un traitement.<br data-start=\"812\" data-end=\"815\" \/>Elle est destin\u00e9e \u00e0 une utilisation <strong data-start=\"851\" data-end=\"874\">hors incubateur CO\u2082<\/strong> et <strong data-start=\"878\" data-end=\"917\">non adapt\u00e9e aux incubations longues<\/strong>.<\/p>\n<h3 data-start=\"920\" data-end=\"949\"><strong data-start=\"924\" data-end=\"949\">Composition du PBS 1X<\/strong><\/h3>\n<p data-start=\"950\" data-end=\"980\">Pour 1000 ml d\u2019eau distill\u00e9e :<\/p>\n<ul data-start=\"981\" data-end=\"1066\">\n<li data-start=\"981\" data-end=\"1000\">\n<p data-start=\"983\" data-end=\"1000\">8.0 g\/L de NaCl<\/p>\n<\/li>\n<li data-start=\"1001\" data-end=\"1019\">\n<p data-start=\"1003\" data-end=\"1019\">0.2 g\/L de KCl<\/p>\n<\/li>\n<li data-start=\"1020\" data-end=\"1043\">\n<p data-start=\"1022\" data-end=\"1043\">1.42 g\/L de Na\u2082HPO\u2084<\/p>\n<\/li>\n<li data-start=\"1044\" data-end=\"1066\">\n<p data-start=\"1046\" data-end=\"1066\">0.24 g\/L de KH\u2082PO\u2084<\/p>\n<\/li>\n<\/ul>\n<h3 data-start=\"1068\" data-end=\"1082\"><strong data-start=\"1072\" data-end=\"1082\">Astuce<\/strong><\/h3>\n<p data-start=\"1083\" data-end=\"1408\"><a href=\"https:\/\/www.em-grade.com\/fr\/tampons\/les-tampons-en-microscopie\/\">Une solution tampon permet de maintenir un pH stable malgr\u00e9 l\u2019ajout de petites quantit\u00e9s d\u2019acide ou de base, ou malgr\u00e9 une dilution.<\/a><\/p>\n<p data-start=\"1083\" data-end=\"1408\">La capacit\u00e9 tampon du PBS repose sur le couple dihydrog\u00e9nophosphate \/ hydrog\u00e9nophosphate (pKa = 7,2), l\u2019un des trois principaux m\u00e9canismes de r\u00e9gulation du pH sanguin (HPO\u2084\u00b2\u207b + H\u207a \u2192 H\u2082PO\u2084\u207b).<\/p>\n<p data-start=\"1410\" data-end=\"1426\">Stockage<\/p>\n<ul data-start=\"1427\" data-end=\"1524\">\n<li data-start=\"1427\" data-end=\"1460\">\n<p data-start=\"1429\" data-end=\"1460\">Temp\u00e9rature ambiante (RT)<\/p>\n<\/li>\n<li data-start=\"1461\" data-end=\"1524\">\n<p data-start=\"1463\" data-end=\"1524\">Note : la solution peut cristalliser \u00e0 basse temp\u00e9rature.<\/p>\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Description du produit Le PBS (Phosphate-Buffered Saline) est une solution tampon couramment utilis\u00e9e en biochimie. Il s\u2019agit d\u2019une solution physiologique contenant du chlorure de sodium, du phosphate disodique, du phosphate&#8230;<\/p>\n","protected":false},"featured_media":11049,"template":"","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":""},"product_brand":[],"product_cat":[227],"product_tag":[],"class_list":["post-11402","product","type-product","status-publish","has-post-thumbnail","product_cat-tampons","pa_buffer-concentration-015x-fr","pa_buffer-concentration-10x-fr","pa_buffer-concentration-1x-fr","pa_buffer-concentration-2x-fr","pa_buffer-concentration-5x-fr","pa_volume-1000ml-fr","pa_volume-125ml-fr","pa_volume-250ml-fr","pa_volume-500ml-fr","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product\/11402","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media\/11049"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=11402"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product_brand?post=11402"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product_cat?post=11402"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product_tag?post=11402"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}