{"id":3901,"date":"2023-12-04T16:35:33","date_gmt":"2023-12-04T15:35:33","guid":{"rendered":"https:\/\/www.em-grade.com\/product\/glutaraldehyde-2-alcian-blue-in-buffer\/"},"modified":"2024-03-05T10:12:58","modified_gmt":"2024-03-05T09:12:58","slug":"glutaraldehyde-2-bleu-alcian-dans-tampon","status":"publish","type":"product","link":"https:\/\/www.em-grade.com\/fr\/produit\/glutaraldehyde-2-bleu-alcian-dans-tampon\/","title":{"rendered":"Glutarald\u00e9hyde 2%, Bleu alcian dans un tampon 0.1M"},"content":{"rendered":"<ul>\n<li><a href=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/12\/3-s2.0-B9780128132883000148-f14-19-97801281328831.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-6931\" src=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/12\/3-s2.0-B9780128132883000148-f14-19-97801281328831-300x216.jpg\" alt=\"\" width=\"300\" height=\"216\" srcset=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/12\/3-s2.0-B9780128132883000148-f14-19-97801281328831-300x216.jpg 300w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/12\/3-s2.0-B9780128132883000148-f14-19-97801281328831.jpg 366w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a> <a href=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/12\/alcian-glutaraldehyde-cellcoat-on-microvilli.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-6926\" src=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/12\/alcian-glutaraldehyde-cellcoat-on-microvilli-272x300.jpg\" alt=\"\" width=\"239\" height=\"264\" srcset=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/12\/alcian-glutaraldehyde-cellcoat-on-microvilli-272x300.jpg 272w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/12\/alcian-glutaraldehyde-cellcoat-on-microvilli-600x663.jpg 600w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/12\/alcian-glutaraldehyde-cellcoat-on-microvilli-927x1024.jpg 927w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/12\/alcian-glutaraldehyde-cellcoat-on-microvilli-768x848.jpg 768w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/12\/alcian-glutaraldehyde-cellcoat-on-microvilli.jpg 1215w\" sizes=\"auto, (max-width: 239px) 100vw, 239px\" \/><\/a>\u00a0 <a href=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/03\/stain-glycocalyx-Alcian-Blue-glutaraldehyde.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-6918\" src=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/03\/stain-glycocalyx-Alcian-Blue-glutaraldehyde-300x209.jpg\" alt=\"\" width=\"378\" height=\"263\" srcset=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/03\/stain-glycocalyx-Alcian-Blue-glutaraldehyde-300x209.jpg 300w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/03\/stain-glycocalyx-Alcian-Blue-glutaraldehyde-600x419.jpg 600w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/03\/stain-glycocalyx-Alcian-Blue-glutaraldehyde-1024x715.jpg 1024w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/03\/stain-glycocalyx-Alcian-Blue-glutaraldehyde-768x536.jpg 768w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/03\/stain-glycocalyx-Alcian-Blue-glutaraldehyde-1536x1072.jpg 1536w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/03\/stain-glycocalyx-Alcian-Blue-glutaraldehyde.jpg 1748w\" sizes=\"auto, (max-width: 378px) 100vw, 378px\" \/><\/a><\/li>\n<li>Le bleu Alcian peut \u00eatre utilis\u00e9 en microscopie \u00e9lectronique pour marquer sp\u00e9cifiquement les glycoprot\u00e9ines membranaires. (enveloppe cellulaire, glycocalyx).<br \/>\nles radicaux carboxyles alcianophiles (glycosyl\u00e9s)\u00a0sous forme de glycoprot\u00e9ines transmembranaires et de glycolipides.<strong>L\u2019\u00e9tude au microscope \u00e9lectronique<\/strong>\u00a0b\u00e9n\u00e9ficie de l\u2019application du\u00a0<strong>bleu d\u2019Alcian<\/strong>, une technique de coloration vitale. Penchons-nous sur sa signification :<\/p>\n<ol>\n<li><strong>Principe de la concentration critique d\u2019\u00e9lectrolytes<\/strong>\u00a0:\n<ul>\n<li>Les chercheurs utilisent le\u00a0principe critique de la concentration d\u2019\u00e9lectrolytes\u00a0lors de l\u2019utilisation d\u2019Alcian Blue pour colorer les\u00a0prot\u00e9oglycanes du cartilage \u00e9piphysaire du rat\u00a0pour la microscopie \u00e9lectronique\u00a0.<\/li>\n<li>Ce principe consiste \u00e0 faire varier la concentration\u00a0en chlorure de magn\u00e9sium (MgCl\u2082) lors de la\u00a0coloration.<\/li>\n<li>En comparant diff\u00e9rentes conditions, les scientifiques ont un aper\u00e7u de la distribution et de la structure du mat\u00e9riau dans le cartilage.<\/li>\n<\/ul>\n<\/li>\n<li><strong>R\u00e9sultats de coloration<\/strong>\u00a0:\n<ul>\n<li>Lorsque le bleu Alcian est ajout\u00e9 au fixateur, il colore \u00e0 la fois le\u00a0mat\u00e9riel intracellulaire dans les vacuoles et le\u00a0mat\u00e9riau matriciel non collag\u00e8ne.<\/li>\n<li>Le mat\u00e9riel color\u00e9 se pr\u00e9sente sous forme\u00a0de granules arrondis ou polygonaux, s\u2019accumulant autour des cellules dans les\u00a0zones prolif\u00e9ratives et hypertrophi\u00e9es\u00a0du cartilage.<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n<ul>\n<li>Il r\u00e9v\u00e8le les radicaux sucr\u00e9s des prot\u00e9ines glycolys\u00e9s<\/li>\n<li><em><a href=\"https:\/\/link.springer.com\/article\/10.1007\/BF01004558\"><strong>En r\u00e9sum\u00e9, la coloration au bleu d\u2019Alcian,<\/strong> guid\u00e9e par la concentration critique d\u2019\u00e9lectrolytes, fournit des informations pr\u00e9cieuses sur la composition et la structure du cartilage au niveau ultrastructurel dans les \u00e9tudes de microscopie \u00e9lectronique\u00a0<\/a><\/em><\/li>\n<\/ul>\n<\/li>\n<li><strong><u>PROTOCOLE FIXATION\u00a0:<\/u><\/strong><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p><strong>pr\u00eat \u00e0 l&#8217;emploi<\/strong><\/p>\n<p><em><strong>augmente la visibilit\u00e9 des glycoprot\u00e9ines membranaires (cell coat \/ glycocalyx cellulaire)<\/strong><\/em><\/p>\n<p>Eau distill\u00e9e<br \/>\nMicro filtr\u00e9 0,22\u00b5 &#8211; St\u00e9rile<br \/>\nscell\u00e9 sous gaz neutre<br \/>\nQualit\u00e9 EM<\/p>\n","protected":false},"featured_media":6936,"template":"","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":""},"product_brand":[],"product_cat":[186,190],"product_tag":[],"class_list":["post-3901","product","type-product","status-publish","has-post-thumbnail","product_cat-fixateurs","product_cat-fixateurs-specifiques","pa_buffer-type-tampon-cacodylate","pa_buffer-type-tampon-phosphate","pa_glutaraldehyde-concentration-25-fr-2","pa_glutaraldehyde-concentration-2-fr-2","pa_ph-7-fr","pa_ph-72-fr","pa_ph-74-fr","pa_volume-1000ml-fr","pa_volume-10ml-x-10-fr","pa_volume-125ml-fr","pa_volume-250ml-fr","pa_volume-30ml-fr","pa_volume-500ml-fr","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product\/3901","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media\/6936"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=3901"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product_brand?post=3901"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product_cat?post=3901"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product_tag?post=3901"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}