{"id":4180,"date":"2023-12-04T16:34:47","date_gmt":"2023-12-04T15:34:47","guid":{"rendered":"https:\/\/www.em-grade.com\/product\/stain77-emstain-solution\/"},"modified":"2024-02-07T12:00:40","modified_gmt":"2024-02-07T11:00:40","slug":"stain77-solution-coloration-em","status":"publish","type":"product","link":"https:\/\/www.em-grade.com\/fr\/produit\/stain77-solution-coloration-em\/","title":{"rendered":"STAIN77 &#8211; Solution de coloration EM"},"content":{"rendered":"<p>Stain77 est un agent de contraste extr\u00eamement peu toxique, compos\u00e9 de sels de lithium et de tungst\u00e8ne (voir la fiche de donn\u00e9es de s\u00e9curit\u00e9). Il am\u00e9liore efficacement les contrastes positifs et n\u00e9gatifs. Il reste insensible \u00e0 la lumi\u00e8re et permet un stockage \u00e0 long terme \u00e0 temp\u00e9rature ambiante. Si une cristallisation se produit au fil du temps, vous pouvez restaurer les propri\u00e9t\u00e9s d&rsquo;am\u00e9lioration du contraste du produit par filtration ou centrifugation.<\/p>\n<p>Son pH reste stable.<\/p>\n<p>Stain77 est une v\u00e9ritable alternative \u00e0 l&rsquo;ac\u00e9tate d&rsquo;uranyle pour le contraste des coupes et la coloration n\u00e9gative, avec un pH proche de 7, ce qui garantit la s\u00e9curit\u00e9 de l&rsquo;utilisateur. Les r\u00e9sultats du contraste, apr\u00e8s renforcement par le citrate de plomb, sont \u00e9quivalents ou m\u00eame sup\u00e9rieurs \u00e0 ceux de l&rsquo;ac\u00e9tate d&rsquo;uranyle.<\/p>\n<p><strong>Il est disponible en deux variantes et deux volumes (30 ml et 200 ml) :<\/strong><\/p>\n<ul>\n<li>Un flacon Airless de 30 ml avec un distributeur goutte \u00e0 goutte pour la coloration manuelle.<\/li>\n<li>Une option de 200 ml pour une utilisation avec les syst\u00e8mes de contraste automatis\u00e9s<a href=\"https:\/\/www.leica-microsystems.com\/fr\/produits\/preparation-dechantillons-pour-metmeb\/p\/leica-em-ac20\/\">Leica EM AC20<\/a> ou <a href=\"https:\/\/www.google.com\/search?q=rmc+EM+STAINer&amp;tbm=isch&amp;ved=2ahUKEwjEkd6QzIzwAhUHaRQKHaJQDSQQ2-cCegQIABAA&amp;oq=rmc+EM+STAINer&amp;gs_lcp=CgNpbWcQA1C2eFjDkwFg25gBaABwAHgAgAFYiAGgApIBATSYAQCgAQGqAQtnd3Mtd2l6LWltZ8ABAQ&amp;sclient=img&amp;ei=Tap-YIS3CIfSUaKhtaAC&amp;bih=937&amp;biw=1920&amp;safe=active#imgrc=euhYgEr62LyxLM\">RMC<\/a> (rincer bri\u00e8vement avec de l&rsquo;eau distill\u00e9e pendant 2 x 5 minutes).<\/li>\n<\/ul>\n<p><strong>Le protocole de contraste de la section ultramince de Stain77 implique :<\/strong><\/p>\n<ol>\n<li>Appliquer une goutte de Stain77 pendant 1 \u00e0 2 minutes.<\/li>\n<li>S\u00e9chage.<\/li>\n<li>Rincer bri\u00e8vement avec de l&rsquo;eau distill\u00e9e.<\/li>\n<li>Drying.<\/li>\n<li>Contre-coloration au citrate de plomb pendant 1 minute, suivie d&rsquo;un rin\u00e7age \u00e0 l&rsquo;eau distill\u00e9e et d&rsquo;un s\u00e9chage.<\/li>\n<li>Observation au microscope \u00e9lectronique \u00e0 transmission (TEM).<\/li>\n<\/ol>\n<p>Remarque : \u00e9vitez de conserver l&rsquo;eau de rin\u00e7age au r\u00e9frig\u00e9rateur, car l&rsquo;eau froide peut absorber le CO2, ce qui pourrait contaminer vos sections.<\/p>\n<p><a href=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/dessin2-protocole-stain77.png\"><img decoding=\"async\" class=\"alignnone wp-image-1234\" src=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/dessin2-protocole-stain77-300x52.png\" alt=\" largeur=\" height=\"64\" srcset=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/dessin2-protocole-stain77-300x52.png 300w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/dessin2-protocole-stain77.png 594w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p>Le protocole ci-dessus am\u00e9liore le contraste des coupes ultraminces (contraste positif) et convient \u00e9galement \u00e0 la coloration n\u00e9gative. Cependant, une coloration n\u00e9gative ne n\u00e9cessite pas de contre-coloration avec du citrate de plomb apr\u00e8s l&rsquo;utilisation de Stain77.<\/p>\n<p>Stain77 peut \u00eatre conserv\u00e9 en laboratoire \u00e0 temp\u00e9rature ambiante et n&rsquo;est pas affect\u00e9 par l&rsquo;exposition \u00e0 la lumi\u00e8re.<\/p>\n<p>Stain77 peut \u00eatre conserv\u00e9 en laboratoire \u00e0 temp\u00e9rature ambiante et n&rsquo;est pas affect\u00e9 par l&rsquo;exposition \u00e0 la lumi\u00e8re.<\/p>\n<p>&nbsp;<\/p>\n<p><iframe loading=\"lazy\" title=\"YouTube video player\" src=\"https:\/\/www.youtube.com\/embed\/TZ7ztaz0rWQ?si=PnWfH3Bbz-LrsUte\" width=\"560\" height=\"315\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n","protected":false},"excerpt":{"rendered":"<ul>\n<li>Pr\u00eat \u00e0 l&#8217;emploi<\/li>\n<li>Pour les contrastes positifs et n\u00e9gatifs<\/li>\n<li>pH proche de 7<\/li>\n<li>Eau distill\u00e9e<\/li>\n<li>Microfiltr\u00e9e &#8211; St\u00e9rile<\/li>\n<li>Scell\u00e9 sous gaz inerte<\/li>\n<li>Qualit\u00e9 EM Grade<\/li>\n<\/ul>\n","protected":false},"featured_media":3410,"template":"","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":""},"product_brand":[],"product_cat":[228],"product_tag":[],"class_list":["post-4180","product","type-product","status-publish","has-post-thumbnail","product_cat-contrastants","pa_volume-200ml-fr","pa_volume-30ml-fr","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product\/4180","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media\/3410"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=4180"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product_brand?post=4180"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product_cat?post=4180"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product_tag?post=4180"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}