{"id":4236,"date":"2023-12-04T16:34:56","date_gmt":"2023-12-04T15:34:56","guid":{"rendered":"https:\/\/www.em-grade.com\/product\/phosphate-buffer\/"},"modified":"2025-05-15T12:45:55","modified_gmt":"2025-05-15T10:45:55","slug":"tampon-phosphate","status":"publish","type":"product","link":"https:\/\/www.em-grade.com\/fr\/produit\/tampon-phosphate\/","title":{"rendered":"Tampon Phosphate"},"content":{"rendered":"

Le tampon phosphate<\/strong>, parfois d\u00e9sign\u00e9 sous des variantes proches du \u00ab\u00a0tampon de S\u00f6rensen\u00a0\u00bb<\/em>, reste une ressource largement utilis\u00e9e en biologie. Les chercheurs reconnaissent son r\u00f4le essentiel, car il offre une capacit\u00e9 tampon robuste<\/strong> et permet un ajustement du pH dans une plage de 5,8 \u00e0 8,0<\/strong>.<\/p>\n

Des concentrations molaires \u00e9lev\u00e9es (0,4M et plus)<\/strong> emp\u00eachent efficacement la prolif\u00e9ration bact\u00e9rienne, m\u00eame \u00e0 temp\u00e9rature ambiante. Nous recommandons de se procurer des solutions stock \u00e0 0,4M<\/strong> et d\u2019effectuer les dilutions n\u00e9cessaires au cours des exp\u00e9riences. Cette pratique permet de minimiser les risques de contamination<\/strong>, fr\u00e9quents apr\u00e8s quelques jours lorsque la concentration est r\u00e9duite \u00e0 0,1M<\/strong>.<\/p>\n

Nos produits sont rigoureusement s\u00e9lectionn\u00e9s pour leur puret\u00e9<\/strong>. La concentration \u00e0 0,1M est sensible<\/strong> et doit \u00eatre conserv\u00e9e au r\u00e9frig\u00e9rateur \u00e0 4\u00b0C<\/strong>. Si une opacification appara\u00eet, cela peut indiquer une contamination.<\/p>\n

Nos solutions sont scell\u00e9es herm\u00e9tiquement sous gaz neutre (argon)<\/strong>, \u00e9liminant efficacement l\u2019oxyg\u00e8ne pour pr\u00e9venir la prolif\u00e9ration bact\u00e9rienne. \u00c0 l\u2019ouverture du flacon, il est recommand\u00e9 de noter la date<\/strong>, car une exposition \u00e0 l\u2019air peut favoriser la croissance microbienne.<\/p>\n

Avantages & Inconv\u00e9nients<\/h2>\n

\u2705 Avantages<\/h3>\n
    \n
  • Le tampon phosphate mime certains composants des fluides extracellulaires<\/strong>, ce qui en fait l’un des tampons les plus physiologiques<\/strong>.<\/li>\n
  • Non toxique pour les cellules<\/strong> (ne compromet pas la viabilit\u00e9 cellulaire).<\/li>\n
  • Stabilit\u00e9 du pH<\/strong> malgr\u00e9 les variations de temp\u00e9rature.<\/li>\n
  • Stockage \u00e0 4\u00b0C<\/strong> \u2192 stabilit\u00e9 prolong\u00e9e (plusieurs semaines).<\/li>\n<\/ul>\n

    \u274c Inconv\u00e9nients<\/h3>\n
      \n
    • Pr\u00e9cipitation possible<\/strong> avec les ions calcium (pendant la fixation).<\/li>\n
    • Formation de pr\u00e9cipit\u00e9s<\/strong> avec l\u2019ac\u00e9tate d\u2019uranyle et r\u00e9action avec les sels de plomb.<\/li>\n
    • Risque de pr\u00e9cipitation lors de la d\u00e9shydratation \u00e0 l\u2019\u00e9thanol<\/strong> \u2192 privil\u00e9gier l\u2019ac\u00e9tone.<\/li>\n
    • Sensible \u00e0 la contamination<\/strong> \u2192 stockage obligatoire \u00e0 4\u00b0C<\/strong>.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"
        \n
      • Disponible aux pH 6,9 \u00e0 7,4<\/li>\n
      • Fabrication sur demande<\/li>\n
      • Conditionn\u00e9 sous gaz neutre<\/li>\n
      • Fabriqu\u00e9 en France<\/li>\n<\/ul>\n","protected":false},"featured_media":11055,"template":"","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":""},"product_brand":[],"product_cat":[227],"product_tag":[],"class_list":["post-4236","product","type-product","status-publish","has-post-thumbnail","product_cat-tampons","pa_buffer-concentration-01m-fr","pa_buffer-concentration-02m-fr","pa_buffer-concentration-04m-fr","pa_buffer-concentration-05m-fr","pa_volume-1000ml-fr","pa_volume-125ml-fr","pa_volume-250ml-fr","pa_volume-500ml-fr","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product\/4236","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media\/11055"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/media?parent=4236"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product_brand?post=4236"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product_cat?post=4236"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/fr\/wp-json\/wp\/v2\/product_tag?post=4236"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}