new version stain on bloc for AFS, Cryo\u00a0 application. ![\"Formvar](\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/03\/Formvar-solution-05-5-150x150.jpg\")
<\/b><\/span><\/h3>\n<\/h3>\n
Protocol : <\/b>UranyLess<\/b>\u00a0 cryo-Acetone version <\/b>cryo<\/b> for AFS application stain sample on bloc<\/b>:<\/b><\/p>\n
Culture BeWo<\/i> cells on Saphir disc cryofixed by EM HPM 100<\/p>\n![\"Uranyless](\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/05\/uranyless-AFS1-150x150.jpg\")
<\/a>picture of Uranyless acetone\u00a0<\/p><\/div>\n
\n- 8 hours 2% OsO4\/acetone solution at -90 \u00b0C<\/li>\n
- 0.5-hour temperature increase<\/li>\n
- 8 hours 2% OsO4 \/acetone solution at -60 \u00b0C<\/li>\n
- 0.5-hour temperature increase<\/li>\n
- 8 hours 2% OsO4 \/acetone solution at -30 \u00b0C<\/li>\n
- Remove OsO4 solution and add pure acetone<\/li>\n
- 1 hour washing<\/li>\n
- Remove acetone and add UranyLess acetone version cryo for AFS application.<\/li>\n
- 8 hours UranyLess acetone version at -30 \u00b0C (stain on bloc)<\/li>\n
- 1.5 hours temperature increase<\/li>\n
- 0 \u00b0C End of AFS<\/li>\n<\/ul>\n
![\"uranyless](\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/05\/uranyless-cryo-4-150x150.jpg\")
<\/a>picture of microscopy results uranyless acetone\u00a0<\/p><\/div>\n
As soon as 0 \u00b0C was reached, the samples were washed one time with acetone glass distilled and subsequently embedded in Epon resin at room temperature.<\/p>\n
this protocol developed by Delta Microscopies R&D and validated in the laboratory of the Faculty of Medicine of Graz Austria<\/i><\/b><\/p>\n
<\/p>\n
<\/p>\n
\u00a0 \u00a0 <\/a>![\"\"](\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/05\/uranyless-AFS-sans-uL-150x150.jpg\")
\u00a0 <\/a>![\"\"](\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/05\/uranyless-cryo-fixation-without-UL-stain-150x150.jpg\")
<\/a><\/p>\nOsO4 fixation without uranyless acetone staining<\/span>, membrane boundaries are not visible.<\/em><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":" Uranyless cryo-acetone\u00a0 \u00a0 new version stain on bloc for AFS, Cryo\u00a0 application. Protocol : UranyLess\u00a0 cryo-Acetone version cryo for AFS application stain sample on bloc: Culture BeWo cells on…<\/p>\n","protected":false},"author":8,"featured_media":8776,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[782],"tags":[783],"class_list":["post-8774","post","type-post","status-publish","format-standard","has-post-thumbnail","category-em-stain-it","tag-uranyless-afs-application-it"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts\/8774","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/comments?post=8774"}],"version-history":[{"count":1,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts\/8774\/revisions"}],"predecessor-version":[{"id":10833,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts\/8774\/revisions\/10833"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/media\/8776"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/media?parent=8774"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/categories?post=8774"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/tags?post=8774"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
![\"Uranyless](\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/05\/uranyless-AFS1.jpg\")
<\/a>picture of Uranyless acetone\u00a0<\/p><\/div>\n
- \n
- 8 hours 2% OsO4\/acetone solution at -90 \u00b0C<\/li>\n
- 0.5-hour temperature increase<\/li>\n
- 8 hours 2% OsO4 \/acetone solution at -60 \u00b0C<\/li>\n
- 0.5-hour temperature increase<\/li>\n
- 8 hours 2% OsO4 \/acetone solution at -30 \u00b0C<\/li>\n
- Remove OsO4 solution and add pure acetone<\/li>\n
- 1 hour washing<\/li>\n
- Remove acetone and add UranyLess acetone version cryo for AFS application.<\/li>\n
- 8 hours UranyLess acetone version at -30 \u00b0C (stain on bloc)<\/li>\n
- 1.5 hours temperature increase<\/li>\n
- 0 \u00b0C End of AFS<\/li>\n<\/ul>\n<\/a>
picture of microscopy results uranyless acetone\u00a0<\/p><\/div>\n
As soon as 0 \u00b0C was reached, the samples were washed one time with acetone glass distilled and subsequently embedded in Epon resin at room temperature.<\/p>\n
this protocol developed by Delta Microscopies R&D and validated in the laboratory of the Faculty of Medicine of Graz Austria<\/i><\/b><\/p>\n
<\/p>\n
<\/p>\n
\u00a0 \u00a0 <\/a>
\u00a0 <\/a><\/a><\/p>\nOsO4 fixation without uranyless acetone staining<\/span>, membrane boundaries are not visible.<\/em><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"
Uranyless cryo-acetone\u00a0 \u00a0 new version stain on bloc for AFS, Cryo\u00a0 application. Protocol : UranyLess\u00a0 cryo-Acetone version cryo for AFS application stain sample on bloc: Culture BeWo cells on…<\/p>\n","protected":false},"author":8,"featured_media":8776,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[782],"tags":[783],"class_list":["post-8774","post","type-post","status-publish","format-standard","has-post-thumbnail","category-em-stain-it","tag-uranyless-afs-application-it"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts\/8774","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/comments?post=8774"}],"version-history":[{"count":1,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts\/8774\/revisions"}],"predecessor-version":[{"id":10833,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts\/8774\/revisions\/10833"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/media\/8776"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/media?parent=8774"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/categories?post=8774"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/tags?post=8774"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
![\u00a0 \u00a0 <\/a>](\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2024\/03\/Formvar-solution-05-5-scaled.jpg\"){kind=link}