{"id":8781,"date":"2024-04-05T09:19:06","date_gmt":"2024-04-05T07:19:06","guid":{"rendered":"https:\/\/www.em-grade.com\/non-categorizzato\/how-to-choose-the-ph-of-your-buffer-in-fixative-solution-for-electron-microscopy\/"},"modified":"2024-06-12T11:29:44","modified_gmt":"2024-06-12T09:29:44","slug":"how-to-choose-the-ph-of-your-buffer-in-fixative-solution-for-electron-microscopy","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/it\/buffers-it\/how-to-choose-the-ph-of-your-buffer-in-fixative-solution-for-electron-microscopy\/","title":{"rendered":"How to choose the value pH buffer in fixative solution for electron microscopy."},"content":{"rendered":"

\u00a0Potential Hydrogen denoted pH<\/strong>,<\/p>\n

is a measure of the chemical activity of hydrons (also commonly called protons or hydrogen ions) in solution. In particular, in aqueous solution, these ions are present in the form of hydronium ion (the simplest of oxonium ions).\"intra<\/a><\/p>\n

Acid-base balance, or pH homeostasis, is a function of the human body that aims to regulate plasma pH.<\/p>\n

Plasma pH normally ranges from 7.38 to 7.42.<\/p>\n

We speak of acidosis in the event of a decrease in pH and alkalosis in the event of an increase in it.<\/p>\n

In electron microscopy dealing with biological samples (mammals), the pH of the solutions is close to 7 to 7.4.<\/p>\n

you will notice in this diagram the organelles are at different pH,\u00a0 this is why you need to find a compromise pH value close to 7.<\/p>\n

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