{"id":8808,"date":"2024-02-25T11:32:51","date_gmt":"2024-02-25T10:32:51","guid":{"rendered":"https:\/\/www.em-grade.com\/non-categorizzato\/recipe-preparation-pbs-phosphate-buffer-saline-1x-ph-7-4\/"},"modified":"2024-06-12T11:29:57","modified_gmt":"2024-06-12T09:29:57","slug":"recipe-preparation-pbs-phosphate-buffer-saline-1x-ph-7-4","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/it\/buffers-it\/recipe-preparation-pbs-phosphate-buffer-saline-1x-ph-7-4\/","title":{"rendered":"Recipe \/ Preparation : PBS (Phosphate Buffer Saline) 1X, pH 7.4."},"content":{"rendered":"

To prepare\u00a0 500 ml of\u00a0PBS\u00a0 (1X, pH 7.4)<\/p>\n\n\n\n\n\n\n\n
Component<\/td>\nAmount<\/td>\nConcentration<\/td>\n<\/tr>\n
Sodium chloride<\/a>\u00a0(mw:\u00a058.44\u00a0g\/mol)<\/td>\n4\u00a0g<\/td>\n0.137\u00a0M<\/td>\n<\/tr>\n
Potassium Chloride\u00a0(mw:\u00a074.55\u00a0g\/mol)<\/td>\n0.1\u00a0g<\/td>\n\u00a0 \u00a00.0027 M<\/td>\n<\/tr>\n
Sodium Phosphate Dibasic\u00a0(mw:\u00a0141.96\u00a0g\/mol)<\/td>\n0.72\u00a0g<\/td>\n\u00a0 \u00a0 \u00a0 \u00a00.01 M<\/td>\n<\/tr>\n
Potassium Phosphate Monobasic (mw: 136.09 g\/mol)<\/td>\n\u00a0 \u00a0 0.1225 g<\/td>\n0.0018 M<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n


\n\"\"<\/a><\/p>\n

    \n
  1. \u00a0Prepare 450 mL of distilled water in a suitable container.<\/li>\n
  2. Add\u00a04\u00a0g\u00a0of\u00a0Sodium chloride\u00a0to the solution.<\/li>\n
  3. Add\u00a00.1\u00a0g\u00a0of\u00a0Potassium Chloride\u00a0to the solution.<\/li>\n
  4. Add\u00a00.72\u00a0g\u00a0of\u00a0Sodium Phosphate Dibasic\u00a0to the solution.<\/li>\n
  5. Add\u00a00.1225\u00a0g\u00a0of\u00a0Potassium Phosphate Monobasic\u00a0to the solution.<\/li>\n
  6. Adjust solution to desired<\/a> pH \u2248 7.4 or other pH.<\/li>\n
  7. Add distilled water until the volume is 500 ml<\/li>\n<\/ol>\n

    We also offer our ready-to-use PBS buffer available on our shop: PBS Buffer.\u00a0\u00a0<\/a><\/h4>\n","protected":false},"excerpt":{"rendered":"

    To prepare\u00a0 500 ml of\u00a0PBS\u00a0 (1X, pH 7.4) Component Amount Concentration Sodium chloride\u00a0(mw:\u00a058.44\u00a0g\/mol) 4\u00a0g 0.137\u00a0M Potassium Chloride\u00a0(mw:\u00a074.55\u00a0g\/mol) 0.1\u00a0g \u00a0 \u00a00.0027 M Sodium Phosphate Dibasic\u00a0(mw:\u00a0141.96\u00a0g\/mol) 0.72\u00a0g \u00a0 \u00a0 \u00a0 \u00a00.01 M…<\/p>\n","protected":false},"author":8,"featured_media":8809,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[785],"tags":[802],"class_list":["post-8808","post","type-post","status-publish","format-standard","has-post-thumbnail","category-buffers-it","tag-recipe-pbs-it"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts\/8808","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/comments?post=8808"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts\/8808\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/media\/8809"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/media?parent=8808"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/categories?post=8808"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/tags?post=8808"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}