{"id":8810,"date":"2024-02-21T16:58:03","date_gmt":"2024-02-21T15:58:03","guid":{"rendered":"https:\/\/www.em-grade.com\/non-categorizzato\/the-stain77-a-positive-staining-ultrathins-sections-on-tem-grids\/"},"modified":"2024-06-12T11:29:57","modified_gmt":"2024-06-12T09:29:57","slug":"the-stain77-a-positive-staining-ultrathins-sections-on-tem-grids","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/it\/em-stain-it\/the-stain77-a-positive-staining-ultrathins-sections-on-tem-grids\/","title":{"rendered":"Stain77 : A positive staining ultrathins sections on TEM grids."},"content":{"rendered":"

Stain77, new contrasting agent in Electron Microscopy.
\nStain77 is a\u00a0doubly effective contrastant<\/strong>\u00a0on ultrathin sections and in negative staining of isolated particles in electron microscopy.<\/p>\n

\"stain77<\/a>As Uranyless, requiring contact for one minute with the ultrathins sections on a grid, then a simple draining on filter paper followed by second contact on a drop of lead citrate for 20 to 30 seconds finalized by a short wash with distilled water.<\/p>\n

It gives a very soft contrast and perfectly highlights the differents cellular organelles. Combined with post-treatment with lead citrate, it is possible to delineate cellular compartments by perfectly marking the cyto-membranes.<\/p>\n

\"\"<\/a>\u00a0\u00a0\"\"<\/a>\"\"<\/a>Toxicity is almost zero<\/strong>\u00a0and the coloring can be done on the edge of the bench, it is a Lithium salt associated with a very low concentration of Tungsten, its pH is close to 7.<\/p>\n

It is easy to implement and stable over time. It can be used as an alternative to aqueous UranyLess when the latter is not conclusive for your Application.<\/p>\n

We continue to develop new contrastants because as there is no universal fixative, the contrast can always be improved to better highlight all the cellular microstructures on different<\/p>\n","protected":false},"excerpt":{"rendered":"

Stain77, new contrasting agent in Electron Microscopy. Stain77 is a\u00a0doubly effective contrastant\u00a0on ultrathin sections and in negative staining of isolated particles in electron microscopy. As Uranyless, requiring contact for one…<\/p>\n","protected":false},"author":8,"featured_media":8811,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[782],"tags":[803],"class_list":["post-8810","post","type-post","status-publish","format-standard","has-post-thumbnail","category-em-stain-it","tag-stain77-it"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts\/8810","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/comments?post=8810"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts\/8810\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/media\/8811"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/media?parent=8810"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/categories?post=8810"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/tags?post=8810"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}