{"id":9489,"date":"2023-11-23T11:42:59","date_gmt":"2023-11-23T10:42:59","guid":{"rendered":"https:\/\/www.em-grade.com\/non-categorizzato\/how-to-prepare-o-t-e-stain-oolong-solution\/"},"modified":"2023-11-23T11:42:59","modified_gmt":"2023-11-23T10:42:59","slug":"how-to-prepare-o-t-e-stain-oolong-solution","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/it\/em-stain-it\/how-to-prepare-o-t-e-stain-oolong-solution\/","title":{"rendered":"How to prepare O.T.E stain (Oolong) solution ?"},"content":{"rendered":"<p><iframe loading=\"lazy\" title=\"OTE EM staining ( Oolong Tea Extract ) . EM Stain\" width=\"1080\" height=\"608\" src=\"https:\/\/www.youtube.com\/embed\/i7lZhu4Pf9o?start=104&#038;feature=oembed&#038;enablejsapi=1&#038;origin=https:\/\/www.em-grade.com\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" allowfullscreen><\/iframe><\/p>\n<h3><strong><span style=\"color: #000000;\">Protocole<\/span><\/strong><\/h3>\n<p>The preparation of the OTE solution is similar to the infusion of tea water 70\u00b0C not more.<\/p>\n<p>&#8211; Using a heating magnetic stirrer set at 70\u00b0C, dissolve your OTE powder at a concentration of 0.2% not more (because saturation is reached at 0.3%)<\/p>\n<p>&#8211; After complete dissolution (reached in 15 to 30 minutes with stirring at 70 \u00b0 C),<\/p>\n<ol>\n<li>Filter your OTE extract solution at 0.45\u00b5 or better at 0.22\u00b5<\/li>\n<li>Store in a small Eppendorf tube filled to the maximum to reduce air containing oxygen or in an <a href=\"https:\/\/www.em-grade.com\/general\/bottles-with-airless-system\/\">AirLess vial<\/a>.<\/li>\n<li>Store in the darkness at 4 \u00b0C (when handling always let your solutions return to RT)<\/li>\n<li>Contrast your grids with OTE for 15 to 20 minutes, darkness, RT<\/li>\n<li>Drain simply not rinse or very short wash 1 to 2 seconds, after drying<\/li>\n<li>Post-stain 1 to 2 minutes with lead citrate according to Reynolds (Ref # 11300)<\/li>\n<li>Drain, short rinse, dry and ready to observe.<\/li>\n<\/ol>\n<p><b>\u00a0 \u00a0<\/b><\/p>\n<p>Powder OTE , 10gr only by <a href=\"https:\/\/www.em-grade.com\/product\/oolong-tea-extract-ote-powder\/\"><strong>EM-grade.com<\/strong><\/a><\/p>\n<p><a href=\"https:\/\/www.em-grade.com\/product\/oolong-tea-extract-ote-powder\/\"><span style=\"color: #ff0000;\"><b>Code product\u00a0 # GP18-10<\/b><\/span><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Protocole The preparation of the OTE solution is similar to the infusion of tea water 70\u00b0C not more. &#8211; Using a heating magnetic stirrer set at 70\u00b0C, dissolve your OTE&#8230;<\/p>\n","protected":false},"author":8,"featured_media":9490,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[782],"tags":[876],"class_list":["post-9489","post","type-post","status-publish","format-standard","has-post-thumbnail","category-em-stain-it","tag-o-t-e-it"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts\/9489","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/comments?post=9489"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts\/9489\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/media\/9490"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/media?parent=9489"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/categories?post=9489"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/tags?post=9489"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}