{"id":9491,"date":"2023-11-20T15:36:13","date_gmt":"2023-11-20T14:36:13","guid":{"rendered":"https:\/\/www.em-grade.com\/non-categorizzato\/phem-buffer-the-best-buffer-for-immunofluorescence-cell-culture\/"},"modified":"2023-11-20T15:36:13","modified_gmt":"2023-11-20T14:36:13","slug":"phem-buffer-the-best-buffer-for-immunofluorescence-cell-culture","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/it\/buffers-it\/phem-buffer-the-best-buffer-for-immunofluorescence-cell-culture\/","title":{"rendered":"PHEM BUFFER , the best buffer for immunofluorescence cell culture."},"content":{"rendered":"

PHEM buffer<\/strong> was developed for preserving microtubules and is used to preserve cell<\/span> \u00a0<\/span>structure in immunostaining<\/span><\/p>\n

References<\/strong> : Schliwa, Manfred, and Jonathan Van Blerkom. “Structural interaction of cytoskeletal components.” The Journal of Cell Biology 90.1 (1981): 222-235.\u00a0<\/span><\/em><\/p>\n

PHEM buffer is recommended in immunocytochemical, immunostaining studies for tissue and cell cultures.<\/p>\n

\"\" <\/a>\"\"<\/a><\/p>\n

Laboratory work has shown that the PHEM buffer allows better detection of intracellular antigens and less impact on enzymatic and biochemical reactions,
\nwhile PBS is more specific to extracellular membrane antigens,\u00a0for this reason PHEM is preferred during fluorescence markings;
\nThe use of PHEM also ensures excellent preservation of cellular ultrastructure, particularly microtubules. The buffer known as CSK, a derivative of the PHEM formulation is called C<\/strong>ytoSK<\/strong>eletal (CSK) Buffer appears to improve microtubule labeling.<\/p>\n

 <\/p>\n

more informations<\/strong><\/span> PHEM buffer Recipe<\/a><\/h3>\n","protected":false},"excerpt":{"rendered":"

PHEM buffer was developed for preserving microtubules and is used to preserve cell \u00a0structure in immunostaining References : Schliwa, Manfred, and Jonathan Van Blerkom. “Structural interaction of cytoskeletal components.” The…<\/p>\n","protected":false},"author":8,"featured_media":9243,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[785],"tags":[877,878],"class_list":["post-9491","post","type-post","status-publish","format-standard","has-post-thumbnail","category-buffers-it","tag-em-stain-it","tag-phem-it"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts\/9491","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/comments?post=9491"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/posts\/9491\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/media\/9243"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/media?parent=9491"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/categories?post=9491"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/it\/wp-json\/wp\/v2\/tags?post=9491"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}