{"id":9457,"date":"2023-12-04T16:34:47","date_gmt":"2023-12-04T15:34:47","guid":{"rendered":"https:\/\/www.em-grade.com\/product\/ote-stain-oolong-tea-extract-in-cacodylate-buffer-ready-to-use\/"},"modified":"2023-12-04T16:34:47","modified_gmt":"2023-12-04T15:34:47","slug":"ote-stain-oolong-tea-extract-in-cacodylate-buffer-ready-to-use","status":"publish","type":"product","link":"https:\/\/www.em-grade.com\/it\/prodotto\/ote-stain-oolong-tea-extract-in-cacodylate-buffer-ready-to-use\/","title":{"rendered":"OTE Stain (Oolong Tea Extract) in Cacodylate buffer"},"content":{"rendered":"

Product description :<\/strong><\/h4>\n

Uranyl acetate, a contrast agent used in electron microscopy for over 40 years, has faced strict regulations since 2014 due to its toxicity. As a result, researchers have sought less toxic alternatives, leading to the development of non-toxic and non-radioactive contrasting agents like UAR, Uranyless, and OTE.<\/p>\n

OTE (Oolong Tea Extract) is derived from Oolong tea and acts as a tannin, enabling etching completion through a lead citrate post-contrast process. Positioned between green tea (non-oxidized) and black tea (fully oxidized), OTE offers a unique contrast-enhancing solution.<\/p>\n

Application :<\/strong><\/h4>\n

To prepare the OTE solution, dissolve OTE powder in 70\u00b0C water at a concentration of 0.2% using a magnetic stirrer. Complete dissolution occurs within 15 to 30 minutes under agitation, followed by filtering the solution at 0.45\u00b5 or preferably at 0.22\u00b5. Store the solution in a small Eppendorf tube or an AirLess Black bottle in the refrigerator, ensuring darkness at 4\u00b0C. Before use, allow the solution to return to room temperature.<\/p>\n

When contrasting grids with OTE, immerse them in the solution for 15 to 20 minutes at room temperature in darkness. After contrast, drain the grids without rinsing or with a very brief wash (1 to 2 seconds). For post-staining, treat the samples with lead citrate according to Reynolds (Ref # 11300) for 1 to 2 minutes. Subsequently, drain, rinse briefly, dry the samples, and they are ready for observation.<\/p>\n

Additional information :<\/strong><\/h4>\n

The prepared aqueous OTE solution has a slightly acidic pH of 6, which can be adjusted by replacing water with a phosphate or cacodylate Na buffer.<\/p>\n

OTE, being a food product, does not require a Material Safety Data Sheet (MSDS). For visual guidance on the OTE preparation process, refer to the tutorial video: OTE Preparation Video<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"