Introduction to PBS : A Vital Buffer in Biochemistry
Phosphate Buffered Saline (PBS), often abbreviated as PBS, holds a central role in biochemistry. Comprising sodium chloride, disodium phosphate, monopotassium phosphate, and a trace of potassium chloride, it mirrors the physiological solute composition, ensuring isotonicity akin to the human body.
Applications and Usage of PBS
PBS serves as a pivotal solution for cell rinsing, eliminating residual medium before subsequent treatments. Specifically designed for cells outside the CO2 incubator, it is not suitable for extended incubations. Its buffering capacity, hinging on the dihydrogen phosphate/hydrogen phosphate couple (pKa = 7.2), aligns with key mechanisms regulating blood pH.
Widespread Utility in Biological Research
Widely utilized in biological research, Phosphate-Buffered Saline (PBS) offers an isotonic solution crucial in various experimental contexts.
Composition and Storage Recommendations
This PBS formulation includes 1.37 M NaCl, 27 mM KCl, 100 mM Na2HPO4, and 18 mM KH2PO4. To maintain its efficacy, store it at room temperature, but exercise caution, as it may crystallize near 0°C.
Conclusion: PBS – The Trusted Companion in Biochemical Research
Phosphate Buffered Saline (PBS) , synonymous with reliability, stands as an indispensable tool in the realm of biochemical research. Its versatile applications and balanced composition make it a cornerstone solution in laboratories worldwide.