OTE Stain (Oolong Tea Extract) – Powder.

Descrição

Product description :

Our Oolong tea extract (OTE) boasts a high tannin content, ensuring exceptional purity and efficiency in contrast enhancement.

Application :

Preparation and Use of OTE Solution:

1. Dissolve OTE powder in 70°C water at a concentration of 0.2% using a magnetic stirrer.
2. Complete dissolution typically occurs within 15 to 30 minutes under agitation.
3. Filter the OTE extract solution at 0.45µ or preferably at 0.22µ.
4. Store the solution in a small Eppendorf tube or an Airless Black bottle, minimizing oxygen exposure, and refrigerate.
5. Keep the solution in darkness at 4°C, allowing it to return to room temperature before use.
6. Contrast grids with OTE for 15 to 20 minutes at room temperature, in darkness.
7. Drain excess solution without rinsing or with very short washing for 1 to 2 seconds after drying.
8. Post-stain with lead citrate according to Reynolds for 1 to 2 minutes.
9. Drain, rinse briefly, dry, and prepare for observation.

Tips :

The prepared OTE solution is slightly acidic, with a pH of 6. To adjust the pH, water can be replaced with a phosphate or cacodylate Na buffer.

For the protocol on preparing your OTE solution and technical details regarding Oolong powder, refer to the technical notes provided.

Additional information :

No MSDS (Material Safety Data Sheet) is provided as OTE is a food product, ensuring safety and suitability for laboratory use.