{"id":7156,"date":"2023-10-24T12:14:18","date_gmt":"2023-10-24T10:14:18","guid":{"rendered":"https:\/\/www.em-grade.com\/sem-categoria\/tampoes-em-microscopia\/"},"modified":"2024-06-12T11:31:18","modified_gmt":"2024-06-12T09:31:18","slug":"tampoes-em-microscopia","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/pt-pt\/tampoes\/tampoes-em-microscopia\/","title":{"rendered":"Tamp\u00f5es em microscopia"},"content":{"rendered":"<p><a href=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-scaled.jpg\"><img alt=\"\" loading=\"lazy\" decoding=\"async\" class=\"wp-image-1860 alignleft\" src=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-150x150.jpg\" alt=\"\" width=\"234\" height=\"234\" srcset=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-150x150.jpg 150w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-140x140.jpg 140w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-100x100.jpg 100w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-500x500.jpg 500w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-350x350.jpg 350w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-1000x1000.jpg 1000w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/10\/cat-3-buffer-em-grade-800x800.jpg 800w\" sizes=\"auto, (max-width: 234px) 100vw, 234px\" \/><\/a>A prepara\u00e7\u00e3o das amostras para a observa\u00e7\u00e3o em microscopia eletr\u00f3nica, requer as maiores precau\u00e7\u00f5es durante a utiliza\u00e7\u00e3o de v\u00e1rias solu\u00e7\u00f5es de acordo com o seu protocolo em v\u00e1rias etapas, o respeito tamb\u00e9m de v\u00e1rios par\u00e2metros determinantes e muito importantes como:<\/p>\n\r\n<ul class=\"eplus-lPd1kt wp-block-list\">&#13;\n<li>o <strong>pH \/Buffer<\/strong>,<\/li>&#13;\n\r\n\r\n&#13;\n<li><strong>tipo de tamp\u00e3o<\/strong> de acordo com a amostra,<\/li>&#13;\n\r\n\r\n&#13;\n<li><strong>Osmolaridade<\/strong> da mistura de aglutina\u00e7\u00e3o<\/li>&#13;\n\r\n\r\n&#13;\n<li><strong>temperatura<\/strong><\/li>&#13;\n\r\n\r\n&#13;\n<li><strong>dura\u00e7\u00e3o da fixa\u00e7\u00e3o.<\/strong><\/li>&#13;\n<\/ul>\r\n<p>The pH of the different cell compartments can vary, so you need to adjust the pH of your buffer solutions according to your studies. In general, in more than 50% of the cases the buffers remain close from 7 to 7.4 A\u00a0<strong>buffer solution<\/strong>\u00a0maintains approximately the same\u00a0pH\u00a0despite the addition of small amounts of an\u00a0acid\u00a0or\u00a0base, or despite \u00a0dilution.<\/p>\r\n<ul>\r\n<li><strong><a href=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-scaled.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-1145 alignright\" src=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-150x150.jpg\" alt=\"PHEM Buffer\" width=\"272\" height=\"272\" srcset=\"https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-150x150.jpg 150w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-140x140.jpg 140w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-100x100.jpg 100w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-500x500.jpg 500w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-350x350.jpg 350w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-1000x1000.jpg 1000w, https:\/\/www.em-grade.com\/wp-content\/uploads\/2023\/08\/PHEM-4-800x800.jpg 800w\" sizes=\"auto, (max-width: 272px) 100vw, 272px\" \/><\/a>phosphate or PB<\/strong>:\u00a0 A simple phosphate buffer is used ubiquitously in biological experiments, as it can be adapted to a variety of pH levels, including isotonic.\u00a0 This wide range is due to phosphoric acid having 3 dissociation constants<\/li>\r\n<li><strong>Fosfato salino ou PBS:<\/strong> O PBS tem muitas utiliza\u00e7\u00f5es porque \u00e9 isot\u00f3nico e n\u00e3o \u00e9 t\u00f3xico para a maioria das c\u00e9lulas. Estas utiliza\u00e7\u00f5es incluem a dilui\u00e7\u00e3o da subst\u00e2ncia e a lavagem das c\u00e9lulas. O PBS com EDTA \u00e9 tamb\u00e9m utilizado para libertar c\u00e9lulas aderentes e agrupadas. Este tamp\u00e3o \u00e9 utilizado em cultura de c\u00e9lulas em atmosfera controlada em incubadoras com 5% de CO2.   <\/li>\r\n<li><strong>D-PBS<\/strong>: <strong>o DPBS<\/strong> \u00e9 o <strong>PBS de Dulbecco<\/strong> e tamb\u00e9m conhecido como CMF-PBS (isento de c\u00e1lcio e magn\u00e9sio, ou <strong>C<\/strong>a <strong>M<\/strong>g <strong>F<\/strong>ree). Sem Ca Mg).<\/li>\r\n<li><strong>cacodilatos<\/strong> : O tamp\u00e3o cacodilato de s\u00f3dio [Na (CH3) 2 AsO2 &#8211; 3H2O] \u00e9 uma alternativa ao tamp\u00e3o fosfato de S\u00f8rensen. Tem uma boa capacidade de tamponamento do pH na gama de pH 5,0-7,4. O cacodilato foi introduzido em aplica\u00e7\u00f5es de microscopia eletr\u00f3nica por Sabatini et al. (1962) como um m\u00e9todo para evitar a adi\u00e7\u00e3o de fosfatos adicionais \u00e0s prepara\u00e7\u00f5es de amostras. As mitoc\u00f4ndrias e outros organelos podem ser danificados quando expostos a concentra\u00e7\u00f5es elevadas de fosfatos presentes nos tamp\u00f5es de S\u00f8rensen. Al\u00e9m disso, o cacodilato n\u00e3o reage com fixadores de alde\u00eddos como acontece com os tamp\u00f5es que cont\u00eam aminas (por exemplo, Tris). A sua efic\u00e1cia em solu\u00e7\u00f5es aglutinantes pode ser o resultado do efeito inibidor do metabolismo do arseniato e n\u00e3o de uma capacidade tamponante especial. Os tamp\u00f5es de Good, ou reagentes formadores de tamp\u00f5es de Good, s\u00e3o uma lista de 20 produtos qu\u00edmicos formadores de tamp\u00f5es seleccionados por Norman Good e a sua equipa, de 1966 a 1980, pela sua adequa\u00e7\u00e3o para utiliza\u00e7\u00e3o em bioqu\u00edmica. Estes tamp\u00f5es s\u00e3o designados por &#8220;Good&#8217;s Buffers&#8221;. Vou citar alguns deles utilizados em Microscopia Eletr\u00f3nica.        <\/li>\r\n<\/ul>\r\n<ul>\r\n<li><strong>HEPES <\/strong><\/li>\r\n<li><strong>MOPS<\/strong><\/li>\r\n<li><strong>Tris , &#8230;<\/strong><\/li>\r\n<\/ul>\r\n","protected":false},"excerpt":{"rendered":"<p>A prepara\u00e7\u00e3o das amostras para a observa\u00e7\u00e3o em microscopia eletr\u00f3nica, requer as maiores precau\u00e7\u00f5es durante a utiliza\u00e7\u00e3o de v\u00e1rias solu\u00e7\u00f5es de acordo com o seu protocolo em v\u00e1rias etapas, o&#8230;<\/p>\n","protected":false},"author":8,"featured_media":7159,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[448],"tags":[650],"class_list":["post-7156","post","type-post","status-publish","format-standard","has-post-thumbnail","category-tampoes","tag-tampoes"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts\/7156","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/comments?post=7156"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts\/7156\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/media\/7159"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/media?parent=7156"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/categories?post=7156"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/tags?post=7156"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}