{"id":7252,"date":"2023-11-10T11:14:41","date_gmt":"2023-11-10T10:14:41","guid":{"rendered":"https:\/\/www.em-grade.com\/sem-categoria\/o-tampao-imidazol-permite-a-retencao-de-lipidos\/"},"modified":"2023-11-10T11:14:41","modified_gmt":"2023-11-10T10:14:41","slug":"o-tampao-imidazol-permite-a-retencao-de-lipidos","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/pt-pt\/tampoes\/o-tampao-imidazol-permite-a-retencao-de-lipidos\/","title":{"rendered":"O tamp\u00e3o imidazol permite a reten\u00e7\u00e3o de l\u00edpidos"},"content":{"rendered":"

O tamp\u00e3o imidazol permite a reten\u00e7\u00e3o dos l\u00edpidos intracelulares mais dif\u00edceis de preservar<\/strong>.<\/p>\n

O tamp\u00e3o imidazol permite a reten\u00e7\u00e3o de l\u00edpidos<\/u><\/strong>. associated with osmium during fixation in 0.1M buffer<\/p>\n

aqui est\u00e1 um protocolo padr\u00e3o<\/p>\n

    \n
  1. Glutaralde\u00eddo ‘2 a 2,5% em tamp\u00e3o imidazol 0,1M<\/strong> pH7,5 – RT<\/li>\n
  2. Lavar em tamp\u00e3o de imidazol 0,2M<\/strong> pH7,5 (2 banhos 3 min) em RT<\/li>\n
  3. \u00d3smio 1% a 2% em tamp\u00e3o imidazol 0,1<\/strong> pH 7,5, 30 min – RT<\/li>\n
  4. \u00daltima lavagem igual ao ponto 2- (Lavagem em tamp\u00e3o de imidazol 0,2M pH7,5 (2 banhos n\u00e3o mais, 3 min) em RT<\/li>\n<\/ol>\n

    Substituir os seus tamp\u00f5es Sorensen PBS ou cacodilato habituais por imidazol , quando o seu estudo d\u00e1 import\u00e2ncia \u00e0s inclus\u00f5es lip\u00eddicas.<\/p>\n

    nota importante: se quiser preservar o conte\u00fado das c\u00e9lulas fixadas, evite lavar demasiado, uma lavagem \u00e9 suficiente; se os l\u00edpidos simples forem os mais dif\u00edceis de preservar, a lavagem deve mesmo ser evitada.<\/p>\n

    \"\" <\/a> \"conserva\u00e7\u00e3o<\/a><\/p>\n

    c\u00e9lula de hepat\u00f3citos<\/p>\n","protected":false},"excerpt":{"rendered":"

    O tamp\u00e3o imidazol permite a reten\u00e7\u00e3o dos l\u00edpidos intracelulares mais dif\u00edceis de preservar. O tamp\u00e3o imidazol permite a reten\u00e7\u00e3o de l\u00edpidos. associated with osmium during fixation in 0.1M buffer aqui…<\/p>\n","protected":false},"author":8,"featured_media":7254,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[448],"tags":[676,675],"class_list":["post-7252","post","type-post","status-publish","format-standard","has-post-thumbnail","category-tampoes","tag-lipidos","tag-tampao-de-imidazol"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts\/7252","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/comments?post=7252"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts\/7252\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/media\/7254"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/media?parent=7252"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/categories?post=7252"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/tags?post=7252"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}