{"id":7334,"date":"2023-11-17T11:33:02","date_gmt":"2023-11-17T10:33:02","guid":{"rendered":"https:\/\/www.em-grade.com\/sem-categoria\/em-stain-ote-oolong-tea-utiliza-a-coloracao-de-seccoes-ultrafinas\/"},"modified":"2023-11-17T11:33:02","modified_gmt":"2023-11-17T10:33:02","slug":"em-stain-ote-oolong-tea-utiliza-a-coloracao-de-seccoes-ultrafinas","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/pt-pt\/mancha-em\/em-stain-ote-oolong-tea-utiliza-a-coloracao-de-seccoes-ultrafinas\/","title":{"rendered":"EM Stain OTE (OOLONG TEA) utiliza a colora\u00e7\u00e3o de sec\u00e7\u00f5es ultrafinas."},"content":{"rendered":"

Prepara\u00e7\u00e3o e utiliza\u00e7\u00e3o do O.T.E.<\/p>\n

A prepara\u00e7\u00e3o da solu\u00e7\u00e3o OTE \u00e9 semelhante \u00e0 infus\u00e3o de \u00e1gua de ch\u00e1 a 70\u00b0C ou de tamp\u00e3o 0,1M.(*)<\/p>\n

– Utilizando um agitador magn\u00e9tico de aquecimento regulado para 70\u00b0C, dissolva o seu p\u00f3 OTE numa concentra\u00e7\u00e3o de 0,2% (porque a satura\u00e7\u00e3o \u00e9 atingida a 0,3%)<\/p>\n

– Ap\u00f3s dissolu\u00e7\u00e3o completa (15 a 30 minutos com agita\u00e7\u00e3o a 70 \u00b0 C),<\/p>\n

    \n
  1. Filtrar a solu\u00e7\u00e3o de extrato de OTE a 0,45\u00b5.<\/li>\n
  2. Armazenar num pequeno tubo Eppendorf cheio at\u00e9 ao m\u00e1ximo para reduzir o ar que cont\u00e9m oxig\u00e9nio ou num frasco AirLess.<\/li>\n
  3. Armazenar no escuro a 4 \u00b0C (ao manusear, deixar sempre as solu\u00e7\u00f5es voltarem \u00e0 temperatura ambiente)<\/li>\n
  4. Contrastar as suas grelhas com OTE durante 15 a 25 minutos, na obscuridade, RT<\/li>\n
  5. Escorrer simplesmente sem enxaguar<\/li>\n
  6. P\u00f3s-mancha de 1 a 2 minutos com citrato de chumbo de acordo com Reynolds (Ref. n.\u00ba 11300 da Delta Microscopies)<\/li>\n
  7. Escorra, passe por \u00e1gua brevemente, seque e est\u00e1 pronto a ver.<\/li>\n<\/ol>\n

    *Nota: a solu\u00e7\u00e3o pode ser conservada durante uma semana, no m\u00e1ximo, mas mais tempo numa solu\u00e7\u00e3o tamp\u00e3o de cacodilato, frasco AirLess (sem crescimento bacteriano).<\/p>\n

    \"\" <\/a>\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

    Prepara\u00e7\u00e3o e utiliza\u00e7\u00e3o do O.T.E. A prepara\u00e7\u00e3o da solu\u00e7\u00e3o OTE \u00e9 semelhante \u00e0 infus\u00e3o de \u00e1gua de ch\u00e1 a 70\u00b0C ou de tamp\u00e3o 0,1M.(*) – Utilizando um agitador magn\u00e9tico de…<\/p>\n","protected":false},"author":8,"featured_media":7268,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[450],"tags":[690,689],"class_list":["post-7334","post","type-post","status-publish","format-standard","has-post-thumbnail","category-mancha-em","tag-extrato-de-cha-oolong","tag-ote-pt-pt"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts\/7334","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/comments?post=7334"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts\/7334\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/media\/7268"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/media?parent=7334"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/categories?post=7334"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/tags?post=7334"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}