{"id":7340,"date":"2023-11-20T15:36:13","date_gmt":"2023-11-20T14:36:13","guid":{"rendered":"https:\/\/www.em-grade.com\/sem-categoria\/phem-buffer-o-melhor-tampao-para-cultura-de-celulas-de-imunofluorescencia\/"},"modified":"2023-11-20T15:36:13","modified_gmt":"2023-11-20T14:36:13","slug":"phem-buffer-o-melhor-tampao-para-cultura-de-celulas-de-imunofluorescencia","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/pt-pt\/tampoes\/phem-buffer-o-melhor-tampao-para-cultura-de-celulas-de-imunofluorescencia\/","title":{"rendered":"PHEM BUFFER , o melhor tamp\u00e3o para cultura de c\u00e9lulas de imunofluoresc\u00eancia."},"content":{"rendered":"

O tamp\u00e3o PHEM<\/strong> foi desenvolvido para preservar os microt\u00fabulos e \u00e9 utilizado para preservar as c\u00e9lulas<\/span> <\/span>estrutura na imunocolora\u00e7\u00e3o<\/span><\/p>\n

Refer\u00eancias<\/strong> : Schliwa, Manfred, e Jonathan Van Blerkom. “Structural interaction of cytoskeletal components.” The Journal of Cell Biology 90.1 (1981): 222-235. <\/span><\/em><\/p>\n

O tamp\u00e3o PHEM \u00e9 recomendado em estudos imunocitoqu\u00edmicos e de imunocolora\u00e7\u00e3o para culturas de tecidos e c\u00e9lulas.<\/p>\n

\"\" <\/a>\"\"<\/a><\/p>\n

O trabalho de laborat\u00f3rio demonstrou que o tamp\u00e3o PHEM permite uma melhor dete\u00e7\u00e3o de antig\u00e9nios intracelulares e um menor impacto nas reac\u00e7\u00f5es enzim\u00e1ticas e bioqu\u00edmicas, enquanto o PBS \u00e9 mais espec\u00edfico para os antig\u00e9nios de membrana extracelular, raz\u00e3o pela qual o PHEM \u00e9 preferido durante as marca\u00e7\u00f5es de fluoresc\u00eancia;<\/p>\n

A utiliza\u00e7\u00e3o de PHEM assegura tamb\u00e9m uma excelente preserva\u00e7\u00e3o da ultra-estrutura celular, em particular dos microt\u00fabulos. O tamp\u00e3o conhecido como CSK, um derivado da formula\u00e7\u00e3o PHEM, \u00e9 designado por tamp\u00e3o C<\/strong>ytoSK<\/strong>eletal (CSK) e parece melhorar a marca\u00e7\u00e3o dos microt\u00fabulos. <\/p>\n

 <\/p>\n

mais informa\u00e7\u00f5es<\/strong><\/span> PHEM buffer Receita<\/a><\/h3>\n","protected":false},"excerpt":{"rendered":"

O tamp\u00e3o PHEM foi desenvolvido para preservar os microt\u00fabulos e \u00e9 utilizado para preservar as c\u00e9lulas estrutura na imunocolora\u00e7\u00e3o Refer\u00eancias : Schliwa, Manfred, e Jonathan Van Blerkom. “Structural interaction of…<\/p>\n","protected":false},"author":8,"featured_media":7276,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[448],"tags":[697,698],"class_list":["post-7340","post","type-post","status-publish","format-standard","has-post-thumbnail","category-tampoes","tag-em-stain-pt-pt","tag-phem-pt-pt"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts\/7340","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/comments?post=7340"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts\/7340\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/media\/7276"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/media?parent=7340"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/categories?post=7340"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/tags?post=7340"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}