{"id":7347,"date":"2023-11-23T11:42:59","date_gmt":"2023-11-23T10:42:59","guid":{"rendered":"https:\/\/www.em-grade.com\/sem-categoria\/como-preparar-a-solucao-de-coloracao-o-t-e-oolong\/"},"modified":"2023-11-23T11:42:59","modified_gmt":"2023-11-23T10:42:59","slug":"como-preparar-a-solucao-de-coloracao-o-t-e-oolong","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/pt-pt\/mancha-em\/como-preparar-a-solucao-de-coloracao-o-t-e-oolong\/","title":{"rendered":"Como preparar a solu\u00e7\u00e3o de colora\u00e7\u00e3o O.T.E. (Oolong)?"},"content":{"rendered":"<p><iframe loading=\"lazy\" title=\"OTE EM staining ( Oolong Tea Extract ) . EM Stain\" width=\"1080\" height=\"608\" src=\"https:\/\/www.youtube.com\/embed\/i7lZhu4Pf9o?start=104&#038;feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" allowfullscreen><\/iframe><\/p>\n<h3><strong><span style=\"color: #000000;\">Protocolo<\/span><\/strong><\/h3>\n<p>A prepara\u00e7\u00e3o da solu\u00e7\u00e3o OTE \u00e9 semelhante \u00e0 infus\u00e3o de \u00e1gua de ch\u00e1 a 70\u00b0C ou mais.<\/p>\n<p>&#8211; Utilizando um agitador magn\u00e9tico de aquecimento regulado para 70\u00b0C, dissolva o seu p\u00f3 de OTE a uma concentra\u00e7\u00e3o de 0,2% ou mais (porque a satura\u00e7\u00e3o \u00e9 atingida a 0,3%)<\/p>\n<p>&#8211; Ap\u00f3s dissolu\u00e7\u00e3o completa (alcan\u00e7ada em 15 a 30 minutos com agita\u00e7\u00e3o a 70 \u00b0 C),<\/p>\n<ol>\n<li>Filtrar a sua solu\u00e7\u00e3o de extrato OTE a 0,45\u00b5 ou melhor a 0,22\u00b5<\/li>\n<li>Armazenar num pequeno tubo Eppendorf cheio at\u00e9 ao m\u00e1ximo para reduzir o ar que cont\u00e9m oxig\u00e9nio ou num <a href=\"https:\/\/www.em-grade.com\/microscopy-advice\/bottles-with-airless-system\/\">frasco AirLess<\/a>.<\/li>\n<li>Armazenar no escuro a 4 \u00b0C (ao manusear, deixar sempre as solu\u00e7\u00f5es voltarem \u00e0 temperatura ambiente)<\/li>\n<li>Contrastar as suas grelhas com OTE durante 15 a 20 minutos, no escuro, RT<\/li>\n<li>Escorrer simplesmente sem enxaguar ou lavar muito pouco tempo, 1 a 2 segundos, depois de secar<\/li>\n<li>P\u00f3s-mancha de 1 a 2 minutos com citrato de chumbo de acordo com Reynolds (Ref. # 11300)<\/li>\n<li>Escorrer, enxaguar brevemente, secar e estar pronto a observar.<\/li>\n<\/ol>\n<p><b>   <\/b><\/p>\n<p>P\u00f3 OTE , 10gr apenas por <a href=\"https:\/\/www.em-grade.com\/product\/oolong-tea-extract-ote-powder\/\"><strong>EM-grade.com<\/strong><\/a><\/p>\n<p><a href=\"https:\/\/www.em-grade.com\/product\/oolong-tea-extract-ote-powder\/\"><span style=\"color: #ff0000;\"><b>C\u00f3digo do produto # GP18-10<\/b><\/span><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Protocolo A prepara\u00e7\u00e3o da solu\u00e7\u00e3o OTE \u00e9 semelhante \u00e0 infus\u00e3o de \u00e1gua de ch\u00e1 a 70\u00b0C ou mais. &#8211; Utilizando um agitador magn\u00e9tico de aquecimento regulado para 70\u00b0C, dissolva o&#8230;<\/p>\n","protected":false},"author":8,"featured_media":7348,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[450],"tags":[703],"class_list":["post-7347","post","type-post","status-publish","format-standard","has-post-thumbnail","category-mancha-em","tag-o-t-e-pt-pt"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts\/7347","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/comments?post=7347"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts\/7347\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/media\/7348"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/media?parent=7347"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/categories?post=7347"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/tags?post=7347"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}