{"id":7358,"date":"2024-01-19T17:03:56","date_gmt":"2024-01-19T16:03:56","guid":{"rendered":"https:\/\/www.em-grade.com\/sem-categoria\/receita-de-tampao-phem\/"},"modified":"2024-01-19T17:03:56","modified_gmt":"2024-01-19T16:03:56","slug":"receita-de-tampao-phem","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/pt-pt\/tampoes\/receita-de-tampao-phem\/","title":{"rendered":"Receita de tamp\u00e3o PHEM"},"content":{"rendered":"

Receita de tamp\u00e3o PHEM<\/em><\/h2>\n

O tamp\u00e3o PHEM tem sido utilizado principalmente para tecidos e culturas de c\u00e9lulas que est\u00e3o a ser processados para estudos imunocitoqu\u00edmicos. A maioria dos antig\u00e9nios, especialmente os intracelulares, coram melhor utilizando PHEM do que os processados utilizando PBS, que tamb\u00e9m tem um efeito mais limitado nas reac\u00e7\u00f5es bioqu\u00edmicas e nas enzimas. A excelente preserva\u00e7\u00e3o da ultra-estrutura celular, nomeadamente dos microt\u00fabulos, \u00e9 tamb\u00e9m uma vantagem da utiliza\u00e7\u00e3o da PHEM.<\/p>\n

\"\"<\/a><\/strong><\/p>\n

Refer\u00eancias:<\/strong><\/p>\n

    \n
  1. Schliwa, Manfred, and Jonathan Van Blerkom<\/strong><\/em>. “Structural interaction of cytoskeletal components.” The Journal of Cell Biology 90.1 (1981): 222-235.<\/li>\n
  2. Jacqueline Montanaro<\/em><\/strong>: Melhoria da ultra-estrutura de invertebrados marinhos utilizando tamp\u00f5es n\u00e3o t\u00f3xicos<\/li>\n<\/ol>\n

    <\/h4>\n

    Receita de prepara\u00e7\u00e3o para 500 ml de tamp\u00e3o PHEM a 0,2M <\/u><\/strong><\/span><\/h4>\n

    Dissolver em 400 ml de \u00e1gua destilada os reagentes em p\u00f3 pela seguinte ordem:<\/em><\/p>\n\n\n\n\n\n\n\n\n
    P\u00f3 reagente<\/strong><\/td>\nN\u00famero de C.A.S<\/strong><\/td>\npeso grama<\/strong><\/td>\nRef. em-grade<\/strong><\/td>\n<\/tr>\n
    HEPES<\/strong><\/td>\n7365-45-9<\/td>\n0.598 gr.<\/td>\nGP19<\/a><\/strong><\/td>\n<\/tr>\n
    EGTA<\/strong><\/td>\n67-42-5<\/td>\n0.380 gr.<\/td>\nGP27<\/strong><\/td>\n<\/tr>\n
    MgSO4 , 7H2O<\/strong><\/td>\n10034-99-8<\/td>\n0.098 gr<\/td>\nGP28<\/strong><\/td>\n<\/tr>\n
    P.I.P.E.S.<\/strong><\/td>\n5625-37-6<\/td>\n1.814 gr<\/td>\nGP21<\/a><\/strong><\/td>\n<\/tr>\n
    WATER D<\/em>.<\/td>\nCompletar o volume final at\u00e9 500 ml e ajustar o pH desejado com NaOH<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    Filtrar a solu\u00e7\u00e3o at\u00e9 0,22\u00b5.<\/em><\/p>\n

    Tamb\u00e9m oferecemos o nosso tamp\u00e3o PHEM pronto a usar, dispon\u00edvel na nossa loja: Tamp\u00e3o PHEM<\/a>. <\/span><\/h4>\n

    Ref. : GT140000<\/span><\/a><\/span><\/span><\/h4>\n","protected":false},"excerpt":{"rendered":"

    Receita de tamp\u00e3o PHEM O tamp\u00e3o PHEM tem sido utilizado principalmente para tecidos e culturas de c\u00e9lulas que est\u00e3o a ser processados para estudos imunocitoqu\u00edmicos. A maioria dos antig\u00e9nios, especialmente…<\/p>\n","protected":false},"author":8,"featured_media":7280,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"itsec_x_frame_options":"","footnotes":""},"categories":[448],"tags":[709],"class_list":["post-7358","post","type-post","status-publish","format-standard","has-post-thumbnail","category-tampoes","tag-tampao-phem"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts\/7358","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/comments?post=7358"}],"version-history":[{"count":0,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/posts\/7358\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/media\/7280"}],"wp:attachment":[{"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/media?parent=7358"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/categories?post=7358"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.em-grade.com\/pt-pt\/wp-json\/wp\/v2\/tags?post=7358"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}