{"id":7385,"date":"2024-02-25T11:32:51","date_gmt":"2024-02-25T10:32:51","guid":{"rendered":"https:\/\/www.em-grade.com\/sem-categoria\/receita-preparacao-pbs-tampao-fosfato-salino-1x-ph-74\/"},"modified":"2025-05-21T16:00:39","modified_gmt":"2025-05-21T14:00:39","slug":"receita-preparacao-pbs-tampao-fosfato-salino-1x-ph-74","status":"publish","type":"post","link":"https:\/\/www.em-grade.com\/pt-pt\/tampoes\/receita-preparacao-pbs-tampao-fosfato-salino-1x-ph-74\/","title":{"rendered":"Preparo de 500 mL de PBS (Phosphate Buffer Saline) 1X, pH 7,4"},"content":{"rendered":"

Prepara\u00e7\u00e3o <\/span>de <\/span>500 <\/span>mL <\/span>de <\/span>PBS (<\/span>Phosphate <\/span>Buffer <\/span>Saline) <\/span>1X, <\/span>pH <\/span>7,4<\/span><\/strong><\/h3>\n

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Composi\u00e7\u00e3o <\/span>para <\/span>500 <\/span>mL <\/span>de <\/span>PBS <\/span>1X (<\/span>pH <\/span>7,4)<\/span><\/strong><\/h4>\n
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Componente<\/span><\/th>\nQuantidade<\/span><\/th>\nConcentra\u00e7\u00e3o <\/span>final<\/span><\/th>\nMassa <\/span>molar (<\/span>g\/<\/span>mol)<\/span><\/th>\n<\/tr>\n<\/thead>\n
Cloreto <\/span>de <\/span>s\u00f3dio (<\/span>NaCl)<\/span><\/td>\n4,0 <\/span>g<\/span><\/td>\n0,137 <\/span>M<\/span><\/td>\n58,44<\/span><\/td>\n<\/tr>\n
Cloreto <\/span>de <\/span>pot\u00e1ssio (<\/span>KCl)<\/span><\/td>\n0,1 <\/span>g<\/span><\/td>\n0,0027 <\/span>M<\/span><\/td>\n74,55<\/span><\/td>\n<\/tr>\n
Fosfato <\/span>diss\u00f3dico (<\/span>Na\u2082<\/span>HPO\u2084)<\/span><\/td>\n0,72 <\/span>g<\/span><\/td>\n0,01 <\/span>M<\/span><\/td>\n141,96<\/span><\/td>\n<\/tr>\n
Fosfato <\/span>monopot\u00e1ssico (<\/span>KH\u2082<\/span>PO\u2084)<\/span><\/td>\n0,1225 <\/span>g<\/span><\/td>\n0,0018 <\/span>M<\/span><\/td>\n136,09<\/span><\/td>\n<\/tr>\n
\u00c1gua <\/span>destilada<\/span><\/td>\nq.<\/span>s.<\/span>p <\/span>500 <\/span>mL<\/span><\/td>\n\u2014<\/span><\/td>\n\u2014<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

Etapas <\/span>de <\/span>preparo <\/span>do <\/span>PBS <\/span>1X (<\/span>500\u202fmL)<\/span><\/strong><\/h3>\n